Method for testing sensitivity of drug by utilizing tissue engineering skin

Abstract

The invention discloses a method for testing sensitivity of an anti-dermatophyte drug by utilizing tissue engineering skin. The method comprises the steps: constructing the tissue engineering skin; respectively adding a test drug and a control drug into the tissue engineering skin; inoculating dermatophytes on the surface of the tissue engineering skin; observing the condition that a skin model is infected by dermatophytes at different time points under a microscope; and finally, analyzing a result and determining the minimum protection concentration which is equivalent to the minimum inhibitory concentration MIC of an in-vitro test. According to the invention, in a mode of testing on the tissue engineering skin, the test drug and the control drug are respectively compared, so that the condition that the skin model is infected by the dermatophyte and the drug protection and antibacterial conditions can be visually observed, the defects of a test tube method and an animal method in pharmacodynamic evaluation of the anti-dermatophyte drug are made up, and drug sensitivity data closer to clinical data can be obtained.

Description

technical field [0001] The invention relates to a method for testing drug sensitivity by using tissue engineered skin, which belongs to the technical field of biomedicine. Background technique [0002] During the research and development of dermatophyte antifungal drugs, sensitivity testing is required. The existing testing methods generally include in vitro and in vivo methods. In vitro methods refer to methods such as test tube methods, through test tubes and related equipment The disadvantage of this method is that it cannot fully test and grasp the characteristics of the drug; the in vivo method refers to methods such as animal methods, which use animals as experimental carriers to test the effectiveness of drugs. The disadvantage is that animals and humans have different responses to drugs, and cannot accurately reflect the effects of drugs. Contents of the invention [0003] In view of the above-mentioned technical problems, the object of the present invention is to...

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Problems solved by technology

[0002] During the research and development of dermatophyte antifungal drugs, sensitivity testing is required. The existing testing methods generally include in vitro and in vivo methods. In vitro methods refer to methods such as test tube methods, through test tubes and related equipment The disadvantage of this method is that it cannot fully test and grasp the characteristics of the drug; the in vivo method refers to methods such as animal methods, which use animals as experimental carriers to test the effectiveness of drugs. The disadvantage is that animals and humans have different responses to drugs, and cannot accurately reflect the effects of drugs.

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