11 results about "Dermodesis" patented technology

The invention belongs to the technical field of biological detection, and particularly relates to a preparation method of lipopolysaccharide-beta-1, 3-glucan binding protein and application of the lipopolysaccharide-beta-1, 3-glucan binding protein in preparation of a broad-spectrum colloidal gold detection product for dermatophytes. The prokaryotic expression system induces the procambarus clarkii-sourced lipopolysaccharide-beta-1, 3-glucan binding protein to be efficiently expressed in an inclusion body form, the optimized inclusion body purification-renaturation process is combined to realize batch preparation, and the colloidal gold chromatography technology is integrated, so that the procambarus clarkii-sourced lipopolysaccharide-beta-1, 3-glucan binding protein is obtained. The bottlenecks of the traditional detection method in sensitivity, broad spectrum and field applicability are broken through.

The disclosure generally relates to cocrystals of posaconazole and a coformer. The disclosure further relates to pharmaceutical compositions comprising the cocrystals, as well as methods of making the cocrystals, and methods of treating or preventing fungal, yeast, or dermatophyte infections using the cocrystals.

The invention discloses a preparation method of a traditional Chinese medicine composition for treating skin tinea, allergic dermatitis and acne, and relates to the field of pharmaceutical preparations, the extraction rate of gallic acid and ethyl gallate contained in total tannin in fructus chebulae flesh, fructus terminaliae billericae flesh and fructus chebulae immaturus is improved through an enzymolysis method, and the transfer rate of effective components of quercetin is improved through a boiling granulation technology; therefore, the effect of treating psoriasis of the composition is improved. By adopting a boiling granulation technology, the technical problems of long preparation period and high cost in the prior art are solved. The release speed of aloe in intestinal tracts is controlled by adopting a slow release technology, and the upper and lower limit content range of barbaloin is controlled to reduce the occurrence rate of adverse reactions of the composition. Meanwhile, butyl hydroxy anisd (BHA), butylated hydroxytoluene (BHT) and a citric acid system are adopted, so that the problems of PEO skeleton degradation, out-of-control drug release behavior and insufficient main drug stability of the barbaloin-PEO sustained release preparation are solved.

The present invention relates to the cosmetic use of phytosterols to protect and / or restore the skin microbiota and / or to prevent and / or reduce the unsightly, unpleasant, and / or uncomfortable manifestations of skin microbiota alteration. It further relates to a cosmetic treatment method comprising the topical application of phytosterols to at least one area of ​​skin, advantageously the scalp, to protect and / or restore the skin microbiota and / or to prevent and / or reduce the unsightly, unpleasant, and / or uncomfortable manifestations of skin microbiota alteration.Finally, it concerns phytosterols for their use in the treatment and / or prevention and / or reduction of the occurrence of a pathology associated with an alteration of the skin microbiota, in particular chosen from the group consisting of acne, psoriasis and bacterial and / or fungal infections, ulcers, herpes, boils, folliculitis, abscesses, sycosis, impetigo, ecthyma, erysipelas, mycoses such as candidiasis or dermatophytoses such as ringworm and / or scabies, pigmentary and / or acne scars as well as their combinations and / or in the treatment and / or prevention of superinfection of wounds and / or in the prevention of spots.

The present application belongs to the technical field of detection reagents, and particularly relates to a dermatophyte detection kit based on a recombinant LGBP-FITC fluorescent probe and application thereof. The present application obtains LGBP-Cys protein by performing C-terminal cysteine site-directed mutation on LGBP protein from Procambarus clarkii; then, the LGBP-Cys protein is directionally coupled with maleimide-activated FITC under mild neutral conditions, so that the problem of protein activity damage caused by traditional random coupling is solved; finally, the coupling product is placed in a composite stabilizing solution containing an anti-quenching agent, so that the stability of the probe and the detection signal-to-noise ratio are significantly improved. The kit is simple to operate, and detection can be completed within 15 minutes. The kit has high sensitivity, high specificity and visualization advantages for common clinical dermatophytes, and is suitable for rapid bedside diagnosis.

The present invention discloses a culture medium for dermatophytes, a preparation method and a method for culturing dermatophytes, and belongs to the field of biotechnology. The culture medium for dermatophytes comprises components including starch, peptone, chloramphenicol, agar, a soluble magnesium salt, a soluble cesium salt, cycloheximide and gentamicin. The preparation method of the culture medium for dermatophytes comprises: stirring and dissolving starch, peptone, agar, a soluble magnesium salt and a soluble cesium salt according to a concentration ratio, sterilizing the mixture, and then adding cycloheximide, gentamicin and chloramphenicol to obtain the culture medium. The present invention also proposes a culture medium for dermatophytes or a method for culturing dermatophytes using the culture medium for dermatophytes, comprising: inoculating dermatophytes into a culture medium and culturing at 28-30°C for more than 3 days; further, the dermatophytes are preferably Microsporum canis. The culture medium can accelerate the colony growth rate of dermatophytes.

The application discloses a traditional Chinese medicine composition for treating skin tinea, which is made of the following components in parts by weight: 20-40 parts of Sophora flavescens, 10-20 parts of Stachys robusta, 10-20 parts of Kochia scoparia, 10-40 parts of Solpugid, 20-40 parts of Sphaeropsidipus, 20-40 parts of Phellodendron, 20-40 parts of Dictamnus dasycarpus, 20-40 parts of Cnidium monnieri, 10-20 parts of Melioidzine, 10-20 parts of Zanthoxylum bungeanum and 10-20 parts of Scophularia ningpoensis. In the traditional Chinese medicine composition, Sophora flavescens, Phellodendron and Dictamnus dasycarpus can clear heat and dry dampness, Stachys robusta, Kochia scoparia, Solpugid, Sphaeropsidipus, Cnidium monnieri and Scophularia ningpoensis have the effect of killing insects and stopping itching; in addition, Melioidzine can invigorate the spleen and dispel wind, and Zanthoxylum bungeanum can increase the transdermal effect of the composition, so that the whole composition can better play the treatment role of clearing heat and dry dampness, killing insects and stopping itching.

Compositions and methods for treatment of onychomycosis and dermatophytoma are presented. In particularly contemplated aspects, high treatment efficacy is achieved in an atraumatic manner. Moreover, due to the high retention of the antifungal agent in the treatment space and advancement of a newly formed healthy nail plate, onycholysis can be reduced or even entirely reversed, thereby preventing recurrence of infection. Still further, contemplated compositions and methods substantially avoid systemic exposure while achieving high local drug concentration in the treatment space.

The invention belongs to the technical field of biological detection, and particularly relates to a skin tinea fungus colloidal gold detection kit based on chicken IgY as well as a preparation method and application of the skin tinea fungus colloidal gold detection kit. According to the present invention, the skin tinea fungus rapid detection kit with characteristics of extremely high sensitivity, extremely strong specificity, excellent stability and low cost is successfully prepared mainly through the organic combination of three technical innovation points such as sulfydryl PEG surface modification, composite sealing and chicken IgY antibody system. The clinical performance is excellent, the sensitivity is 93.3%, the specificity is 96.7%, a high-quality scheme is provided for skin tinea diagnosis, and a new high-performance colloidal gold detection path based on chicken IgY is created.

The invention discloses a primer, a probe, an implementation method and a detection system for qPCR detection of SQLE drug-resistant mutation of dermatophytes, and the method comprises the following steps: identifying a plurality of key mutation sites related to drug resistance by comparing an obtained dermatophytes SQLE gene sequence with a reference sequence in an NCBI database; designing a specific primer, a TaqMan-MGB fluorescent probe and a wild type closed probe modified by the MGB probe based on the mutation site, and verifying the sensitivity and the specificity through the strain level; the method comprises the following steps: constructing a mixed DNA sample model containing drug-resistant strain DNA, wild type strain DNA and human DNA, optimizing a reaction system of single and multiple qPCR detection, and determining an optimal reaction system of qPCR detection. According to the specific primer and probe for qPCR detection of dermatophyte SQLE drug-resistant mutation with specificity meeting requirements and an optimized reaction system for qPCR detection, whether dermatophyte SQLE drug-resistant mutation exists or not can be quickly verified, the sensitivity and specificity of dermatophyte drug-resistant detection are improved, early precise treatment is facilitated, and prognosis of patients is improved.

This invention provides a compound called *Paris polyphylla* saponin A, its pharmaceutical composition, preparation method, and application, belonging to the fields of pharmaceutical technology and cosmetic technology. It provides *Paris polyphylla* saponin A, as well as *Paris polyphylla* saponins A-D and *Paris polyphylla* saponin A in the preparation of drugs for the prevention and treatment of superficial fungal infections of the skin. The above compounds are effective against *Trichophyton rubrum* (…). Trichophyton rubrum Epidermophyton floccosum ( Epidermophyton floccosum ) and Microsporum gypseum ( Microsporum gypseum It has a significant inhibitory effect on dermatophytes such as dermatophytes and can be used to prepare drugs for the prevention and treatment of superficial fungal infections of the skin.