The invention discloses a model for simulating
dermatophyte infection
in vitro and an establishment method and application thereof, and belongs to the technical field of bioengineering. The model forsimulating
dermatophyte infection
in vitro comprises matrix glue, an epidermal analogue embedded in the solidified matrix glue and
dermatophyte hyphae, the epidermal analogue is of a relatively complete
solid sphere structure, and the exterior of the epidermal analogue is a
cell layer which is composed of substrate layer cells and substrate upper layer cells and distributed in a surrounding mode,and a keratinization sample non-
cell layer is arranged inside; hyphae invade basal layer cells outside the sphere structure and do not invade or only invade a small amount of basal layer cells, and donot invade the keratinization sample non-
cell layer inside the sphere structure; the model can effectively simulate the state that dermatophyte infects
normal skin tissue, the infection process of the model can be directly observed under a phase contrast
microscope, the infection situation can be detected through
omics methods such as high-
throughput RNA sequencing, and the model can be effectively used for researching the pathogenic mechanism of dermatophyte infection and screening anti-dermatophyte drugs.