Preparation method of polyvinyl alcohol-acrylamide-agarose hydrogel with high mechanical strength

A technology of agarose hydrogel and polyvinyl alcohol, which is applied in medical science, prosthesis, tissue regeneration, etc., can solve the problems of poor biocompatibility and poor mechanical properties of hydrogel, and improve biocompatibility , enhance mechanical properties, and promote the effect of chondrocyte proliferation

Pending Publication Date: 2021-12-14
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems of poor mechanical properties and poor biocompatibility of polyvinyl alcohol-acrylamide hydrogel, the present invention provides a preparation method of polyvinyl alcohol-acrylamide-agarose (PVA-AAm-AG) hydrogel

Method used

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  • Preparation method of polyvinyl alcohol-acrylamide-agarose hydrogel with high mechanical strength
  • Preparation method of polyvinyl alcohol-acrylamide-agarose hydrogel with high mechanical strength
  • Preparation method of polyvinyl alcohol-acrylamide-agarose hydrogel with high mechanical strength

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] a) Add 10g of PVA, 10g of AAm, 0.5g of agarose, and 0.1g of N,N'-methylenebisacrylamide into 79mL of deionized water, heat in a constant temperature water bath at 90°C, and stir until the PVA is completely dissolved to obtain a mixed solution A.

[0023] b) Put the mixed solution A at room temperature and keep stirring, and lower the temperature to 20°C for later use. Add 100 μL of tetramethylethylenediamine dropwise to the above mixed solution A, add 3 mL of saturated ammonium persulfate solution in an ice-water bath environment, and stir to obtain mixed solution B.

[0024] c) Pour the mixed solution B into the mold, and perform cyclic freezing and thawing. The freezing temperature is -18°C, the freezing time is 12 hours, and the thawing temperature is 15°C, and the thawing time is 6 hours. The number of cycles is 2 times to obtain polyvinyl alcohol-acrylamide hydrogel.

[0025] Based on the PVA-AAm-AG hydrogel prepared by the above steps, the morphology observation,...

Embodiment 2

[0030] a) Add 20g of PVA, 20g of AAm, 5g of agarose, and 0.5g of N,N'-methylenebisacrylamide into 55mL of deionized water, heat in a constant temperature water bath at 99°C, and stir until the PVA is completely dissolved to obtain a mixed solution A.

[0031] b) Place the mixed solution A at room temperature and continue to stir, and lower the temperature to 50°C for later use. Add 200 μL of tetramethylethylenediamine dropwise to the above mixed solution A, add 6 mL of saturated ammonium persulfate solution in an ice-water bath environment, and stir to obtain mixed solution B.

[0032] c) Pour the mixed liquid B into the mold, and perform cyclic freezing and thawing. The freezing temperature is -25°C, the freezing time is 20h, and the thawing temperature is 25°C, and the thawing time is 12h. The number of cycles is 4 times to obtain polyvinyl alcohol-acrylamide hydrogel.

[0033] Based on the PVA-AAm-AG hydrogel prepared by the above steps, the morphology observation, mechani...

Embodiment 3

[0035] a) Add 15g of PVA, 15g of AAm, 2.5g of agarose, and 0.25g of N,N'-methylenebisacrylamide into 67mL of deionized water, heat in a constant temperature water bath at 95°C, and stir until the PVA is completely dissolved to obtain a mixed solution A.

[0036] b) Place the mixed solution A at room temperature and continue to stir until the temperature drops to 35°C for later use. Add 450 μL of tetramethylethylenediamine dropwise to the above mixed solution A, add 4.5 mL of saturated ammonium persulfate solution in an ice-water bath environment, and stir to obtain mixed solution B.

[0037] c) Pour the mixed solution B into the mold, and perform cyclic freezing and thawing. The freezing temperature is -22°C, the freezing time is 16 hours, and the thawing temperature is 20°C, and the thawing time is 9 hours. The number of cycles is 3 times to obtain polyvinyl alcohol-acrylamide hydrogel.

[0038] Based on the PVA-AAm-AG hydrogel prepared by the above steps, the morphology obs...

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Abstract

The invention belongs to the field of articular cartilage substitute materials, and provides a preparation method of polyvinyl alcohol-acrylamide-agarose hydrogel with high mechanical strength. Polyvinyl alcohol and acrylamide are used as matrix materials, and a small amount of agarose is added. Acrylamide forms a first network through a chemical cross-linking agent, and polyvinyl alcohol is physically cross-linked in a refrigeration cycle to form a second network. Agarose can form hydrogen bonds with molecular chains of polyvinyl alcohol and acrylamide, so that the mechanical strength of the hydrogel is further improved. In addition, due to the introduction of agarose, the hydrogel can adhere to cartilage cells and promote cartilage cell proliferation. The hydrogel prepared by the method is expected to be applied to articular cartilage replacement.

Description

technical field [0001] The invention belongs to the field of biomaterials, in particular to the field of substitute materials for articular cartilage damage, and in particular relates to a preparation method of polyvinyl alcohol-acrylamide-agarose hydrogel with high mechanical strength. Background technique [0002] Hydrogel materials have the characteristics of high water content, low friction coefficient, and good chemical stability, and are expected to be used in the repair of biological tissue damage. However, traditional hydrogels are usually composed of a single network of hydrophilic polymers, which have poor mechanical properties and are difficult to meet the high mechanical strength requirements of articular cartilage, which limits the application of hydrogels in the field of articular cartilage replacement materials. At the same time, as a substitute material for articular cartilage, it must also have good biocompatibility. Adding a large number of bioactive ingre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C08L29/04C08L5/12C08F261/04C08F251/00C08F220/56C08F222/38A61L27/26A61L27/52
CPCC08J3/075C08F261/04C08F251/00A61L27/52A61L27/26A61L2430/06C08J2329/04C08J2405/12C08L5/12C08L29/04C08F220/56C08F222/385
Inventor 张伟李津铭李姮吴承伟
Owner DALIAN UNIV OF TECH
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