65 results about "Chondrocyte cell" patented technology

The invention discloses a recombinant VI-type collagen as well as a preparation method and application thereof. The recombinant VI-type collagen comprises a protein gene segment derived from a natural VI-type collagen alpha2 chain, and proteins P0607, P060702 and / or P060703 capable of promoting cartilage cell proliferation activity are expressed. Gene segments derived from a natural type VI collagen alpha2 chain are integrated through a genetic engineering means, sequence optimization and repetition are carried out on the gene segments to enhance the biological activity of the collagen, the recombinant type VI collagen P0607, P060702 and P060703 can be stably and efficiently produced, the recombinant collagen has the effect of promoting chondrocyte proliferation, and the recombinant collagen can be applied to the field of cartilage cell proliferation. An efficient and reliable biological manufacturing way is provided for research, development and production of osteoarthritis medicines.

The invention provides a nanogel for rapid permeation of articular cartilage and responsive release of a growth factor, which is prepared by polymerizing a peptide fragment which can be cut by a substance (mainly enzyme) which can be specifically expressed on the surface of osteoarthritis cells or secreted into a matrix with the growth factor, an acrylamide monomer and a cationic micromolecule monomer. Unreacted monomer molecules are filtered out through an ultrafiltration tube or other filtering systems, so that the nanogel which can rapidly permeate into the deep part of articular cartilage and can be recognized and cut by enzyme highly expressed on the surface of osteoarthritis cells or secreted enzyme to release growth factors is prepared, and the effect of the growth factors can be more effectively exerted; the nanogel has strong cartilage infiltration capacity, can completely infiltrate cartilage with the thickness of 1mm within less than 3 days, can be cut by MT1-MMP protein specifically expressed on the surfaces of osteoarthritis cells, releases growth factors, is specifically combined with growth factor receptors on the surfaces of cartilage cells, and activates downstream signal channels, so that the proliferation of the cartilage cells is promoted, and the cartilage cell proliferation rate is increased. The cartilage matrix secretion is promoted, so that the cartilage matrix thickening effect is realized, and the osteoarthritis is prevented and treated.

The invention discloses an application method for revealing RIPK3 as a pathological index of osteoarthritis based on transcriptomics analysis, and belongs to the technical field of molecular biology and genetic engineering.The method comprises the following steps that S1, a Trizol reagent is used for conducting total RNA extraction on an articular cartilage specimen, and the RIN number gt is selected; 7.0, constructing a sequencing library by using the high-quality RNA sample; s2, carrying out quality inspection on the constructed library, and carrying out mRNA sequencing by using an Illumina NovaseqTM6000 sequencer after all the libraries are qualified in quality inspection; s3, carrying out bioinformatics analysis on data generated by high-throughput sequencing; s4, different parts of the cartilage tissue of the OA patient are labeled with RIPK3 through immunofluorescence, it is proved that expression of the RIPK3 in cartilage cells has strong correlation with the pathology of the OA focus, and the feasibility of taking the RIPK3 as a clinical pathology index is proved.

The invention relates to the technical field of medicines, foods and health-care products, in particular to application of morusin in preparation of a product for treating osteoarthritis. A hydrogen peroxide induced chondrocyte in-vitro model and a D-galactose subcutaneous injection osteoarthritis animal model are used for screening and evaluating the morusin, and it is found that the morusin has the obvious effects of relieving articular cartilage injury, enhancing articular cartilage toughness, delaying the course of osteoarthritis and the like. Wide application prospects are realized in the aspect of preparing products for treating osteoarthritis.

The invention relates to the technical field of biological medicine, in particular to a biomarker for clinical diagnosis of knee osteoarthritis and application of the biomarker. The biomarker for diagnosing the knee osteoarthritis, disclosed by the invention, is a neuronal glial antigen 2, namely NG2. The inventor finds that the soluble NG2 (sNG2) level is remarkably increased in joint fluid of a knee osteoarthritis patient (OA) and is remarkably positively correlated with the minimum joint gap width of the OA and an inflammatory factor TNF-alpha; compared with a healthy control, the sNG2 level in OA serum is remarkably increased; in addition, the TNF-alpha promotes the expression up-regulation of NG2 in OA cartilage cells and the shedding generation of sNG2. Therefore, by detecting sNG2 in serum and synovial fluid of a patient with knee osteoarthritis, the kit can be used for diagnosing knee osteoarthritis, and a new strategy is provided for clinical diagnosis of knee osteoarthritis.

The invention belongs to the technical field of biological medicines, and discloses a B cell and cartilage cell senescence targeting osteoarthritis treatment medicine. The invention provides an application of an anti-MIF antibody or an antigen binding fragment thereof as a B cell targeting immunomodulator in osteoarthritis treatment drugs, the anti-MIF antibody or the antigen binding fragment thereof regulates and controls an MIF / HMGB1 / MMP13 signal axis through a neutralization effect with MIF, so that the MIF and B cells are significantly co-localized, expression of HMGB1 in the B cells is promoted, and the osteoarthritis treatment effect is improved. The senescence of B cells is reduced and the B cells are promoted to regulate or repair phenotype transformation so as to destroy chronic inflammatory circulation and diffusion of pathogenic plasma cells, and the expression of MMP13 in joint tissues is reduced so as to maintain the steady state of cartilage tissues; the preparation method has an excellent application prospect in preparation of an OA intra-articular injection drug which realizes rapid anti-inflammatory and long-term cartilage protection effects and can realize a lasting curative effect through low-frequency drug delivery.

The invention belongs to the technical field of biomedicine, and particularly relates to a nucleic acid combination product for detecting SIAH2, a diagnostic kit for osteoarthritis and application of the diagnostic kit. According to the application, the models are established by using machine learning to analyze various cell death modes, and the application finds that in the models of the four cell death modes, the ferroptosis model has the highest diagnosis efficiency on the OA and identifies the corresponding biomarkers. It is found that SIAH2 is used as a biomarker to regulate occurrence and development of OA through cartilage cells, and a new diagnosis and treatment strategy is provided for OA.

The invention relates to the technical field of biological medicines, and particularly discloses application of emetine hydrochloride in preparation of a medicine for treating osteoarthritis. A cell test shows that the chondrocyte activity can be remarkably improved by an epecinol hydrochloride alkaline solution; animal model tests show that the epecinol hydrochloride alkali solution can maintain the steady state of chondrocyte metabolism, significantly reduce the cartilage injury degree of osteoarthritis mice and relieve osteoarthritis, and provides a new way for improving the osteoarthritis condition. The chemical structural formula of the emetine hydrochloride is shown in the specification.

The present invention relates to the technical field of biomedical materials, and specifically provides a bone and joint biological super-lubricating copolymer and a preparation method thereof. Dopamine is modified with methacrylic anhydride to prepare dopamine methacrylamide, and then a positively charged poly (2-ethyl-2-oxazoline) with good hydrophilicity and hydration lubrication performance and dopamine methacrylamide with adhesion performance are subjected to free radical polymerization to form a super-lubricating copolymer. The positively charged super-lubricating copolymer has good cell affinity, can promote the attachment, growth and differentiation of chondrocytes, and is beneficial to tissue repair and regeneration. The positively charged super-lubricating copolymer has high hydrophilicity, excellent hydration ability and lubricating properties, can adhere to the joint surface, absorb surrounding water molecules to form a hydration layer, effectively reduce surface friction and wear, and the unique adhesion properties of the copolymer and the phenol structure can effectively scavenge free radicals to achieve an anti-inflammatory effect.

The invention discloses a composition containing bovine bone collagen as well as a preparation method and application thereof, and belongs to the technical field of collagen application. The composition containing the bovine bone collagen is obtained by chemical crosslinking of bovine bone collagen magnesium chelate, sodium hyaluronate, chondroitin sulfate and bovine bone collagen through EDC / NHS. The composition containing bovine bone collagen is prepared from the following components in parts by weight: 5 to 10 parts of bovine bone collagen magnesium chelate, 2 to 5 parts of sodium hyaluronate, 0.5 to 2 parts of chondroitin sulfate and 20 to 30 parts of bovine bone collagen. The bovine bone collagen magnesium chelate, the sodium hyaluronate, the chondroitin sulfate and the bovine bone collagen in the composition containing the bovine bone collagen prepared by the invention have the characteristics of good biocompatibility, capability of promoting cell proliferation and differentiation into cartilage cells, and suitability for cartilage repair.

The present disclosure provides compositions and methods of treating osteoarthritis such as osteoarthritis in a subject. The method comprises blocking the opening of Cx43 hemichannel in chondrocytes by, e.g., using a composition comprising an anti-connexin 43 antibody.

The invention relates to the technical field of biomedicine, and particularly discloses application of an exosome overexpressing a CAP gene and an LGR5 gene in preparation of a medicine for treating cartilage inflammation. The exosome for overexpressing the CAP gene and the LGR5 gene has targeting and cartilage protection functions, can be swallowed by cartilage cells and targeted to the cartilage cells, can improve the levels of a gene Col2a1 for cartilage generation and a gene Sox9 for regulating cartilage development, and can reduce the level of a gene Mmp13 for cartilage degradation, so that the exosome can be applied to cartilage protection of cartilage cells. The cartilage abrasion of osteoarthritis mice is relieved, and knee joints are protected, so that cartilage generation can be promoted, cartilage aging is relieved, and cartilage degradation is reduced in a complex inflammation environment. Furthermore, the invention provides the mesenchymal stem cell exocrine for over-expression of CAP / LGR5, a new technical scheme is provided for targeted therapy based on stem cell engineering, and the mesenchymal stem cell exocrine has a wide application prospect.

The present application relates to lipid vacuole (aka LV)-containing lipocartilage cells (aka LCs), and methods for the differentiation of human lipocartilage progenitors into LV-containing LCs. Also disclosed are methods related to purifying, labeling, maturing, and delivering of LV-containing LCs to a subject in need thereof.

The invention relates to application of andrographis paniculata diterpenoid compounds in preparation of FABP4 inhibitors and osteoarthritis related drugs. The research finds that the andrographis paniculata diterpenoid compound can be used as an effective FABP4 inhibitor, can reduce ADAMTS4 overexpression in cartilage cells, and can be used for preparing the anti-osteoarthritis medicine. After normative, scientific and rigorous tests are carried out, the application of the andrographis paniculata diterpenoid compound, the pharmaceutically acceptable salt of the andrographis paniculata diterpenoid compound, the solvate of the andrographis paniculata diterpenoid compound or the andrographis paniculata diterpenoid compound derivative in preparation of the FABP4 inhibitor is provided. Experiments prove that the andrographis paniculata diterpenoid compound can be used as an effective FABP4 inhibitor, can inhibit ADAMTS4 overexpression induced by FABP4, relieve hose degradation and relieve cartilage injury and / or cartilage erosion caused by osteoarthritis, and can be used for preparing the anti-osteoarthritis medicine.

The invention belongs to the technical field of biomedical materials, and particularly relates to a triple-helix self-assembly recombinant humanized I-type collagen fiber implant as well as a preparation method and application of the triple-helix self-assembly recombinant humanized I-type collagen fiber implant. The invention firstly provides a recombinant humanized type I collagen, wherein the recombinant humanized type I collagen has a unique triple helix structure and an excellent self-assembly property; secondly, by optimizing the self-assembly process of the recombinant humanized I-type collagen, the recombinant humanized I-type collagen implant with orderly arranged collagen fibers is prepared, and the implant has the advantages of excellent injectability, durability, high biological safety, no heating and hemolysis risk, low immunogenicity, good biocompatibility, good biocompatibility and good biocompatibility. Adhesion, migration, proliferation and differentiation of human skin fibroblasts are remarkably promoted, adhesion, migration and proliferation of cartilage cells and chondrogenic differentiation of bone marrow mesenchymal stem cells can be remarkably promoted, and the application to the fields of skin, cartilage, bone and the like can be achieved.

It is an object of the present invention a method to obtain a cartilage cell population enriched in cartilage progenitor cells (CPCs), said method comprising culturing said cells in culture at a density between 10 and 500 cells / cm2. In a further aspect, it is here claimed a population of cartilage cells enriched in CPCs obtainable by means of said method, and their use in the treatment of chondral lesions or joint degeneration.

The invention relates to a method for evaluating the biological value of mesenchymal stem cells, and belongs to the technical field of biology. The method comprises the following steps: firstly, respectively collecting conditioned culture media of mesenchymal stem cells from different donor sources and mesenchymal stem cells expressed by silence TSP2, and detecting the concentration of TSP2 in the culture media by adopting an enzyme-linked immunosorbent assay method; furthermore, inflammatory factors are used for inducing cartilage cells, a conditioned culture medium is replaced for culture for 24 h, then the cells are collected, messenger RNA (mRNA) expression change of II-type collagen of the cells is detected, and the correlation between the TSP2 concentration of the conditioned culture medium and the mRNA expression abundance of the II-type collagen of the cartilage cells is established; the TSP2 concentration in the mesenchymal stem cell culture supernatant is measured, the TSP2 concentration can be used for evaluating the biological value of the mesenchymal stem cells in cartilage regeneration and protection in treatment of osteoarthritis and other degenerative diseases, and a reliable basis is provided for standardization and quality control of mesenchymal stem cell treatment.

The invention provides an injectable magnesium-loaded lipid-based super-lubricating hydrogel microsphere, an articular cavity injection preparation and a preparation method of the injectable magnesium-loaded lipid-based super-lubricating hydrogel microsphere. The method comprises the following steps: S1, preparing a magnesium-loaded multilayer vesicular liposome suspension containing magnesium ions; s2, preparing a non-crosslinked magnesium-containing hydrogel; and S3, mixing the magnesium-loaded multilayer vesicular liposome suspension and the magnesium-containing hydrogel to prepare hydrogel liquid drops, and carrying out crosslinking to form the magnesium-loaded lipid-based super-lubricating hydrogel microspheres. The magnesium loading amount of the lipid-based super-lubrication hydrogel microspheres can be greatly improved; on one hand, the biological function provided by magnesium ions can be greatly prolonged, that is, repairing of cartilage by synovial fluid progenitor cells is continuously promoted; on the other hand, the self-lubricating effect of the lipid-based super-lubricating hydrogel microspheres can reduce the secondary wear of the new cartilage; in addition, magnesium ions can remarkably promote expression of cartilage cells PRG4, so that the stable state of the cartilage cells can be maintained, and joint lubrication can be further enhanced.

1. The name of the design product: bone magnetic growth instrument (MT). 2. The use of the design product: for promoting the regeneration and acceleration of chondrocytes by stimulating epiphyseal cells, and subsequent calcification into hard bone to achieve related auxiliary treatment of bone growth. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

The invention discloses an application of sulphaguanidine in preparation of a medicine for treating osteoarthritis. According to the invention, the obvious curative effect of the sulfadimidine (sulfadimidine) in the aspect of treating the osteoarthritis (OA) through intraarticular injection is confirmed for the first time; research results show that the sulphaguanidine not only can remarkably relieve the symptoms of osteoarthritis, but also can improve the metabolic function of cartilage cells, so that the progress of arthritis is slowed down. Traditional treatment methods mainly include intraarticular injection of anesthetic drugs, joint lubrication drugs (such as sodium hyaluronate), glucocorticoid and platelet rich plasma (PRP). However, these methods have limitations, such as significant effects in short periods but may cause side effects in long-term use. As a novel treatment means, sulphaguanidine not only has a good curative effect, but also has small side effects, and provides a new thought for drug treatment of osteoarthritis.

The invention discloses an antler plate oligopeptide as well as a preparation method and application thereof, and relates to the technical field of bioactive peptides. The preparation method comprises the following steps: extracting an antler plate protein crude extract by taking antler plates as a raw material, preparing short peptides with antler plate molecular weight of less than 3k through pepsin and trypsin hydrolysis, and performing Sephadex G-15 gel chromatography to obtain the antler plate short peptides. The prepared antler plate oligopeptide is low in molecular weight and high in hydrophobicity, easily penetrates through a biological membrane barrier to reach an in-vivo effective part, fully plays the biological activity effect of small molecule peptide, can effectively promote C28 / I2 cartilage cell proliferation, can increase the expression of osteoarthritis related phenotype protein Collagen II in an in-vitro model, can reduce the expression of MMP3, MMP13 and ADAMTS-5, and can be used for treating osteoarthritis. Meanwhile, cartilage matrix degradation in an osteoarthritis rat model is inhibited, cartilage injury is improved, and inflammatory response induced by osteoarthritis is reduced.

The utility model discloses an autologous chondrocyte culture device for cartilage tissue repair, which comprises a culture device body and a culture shelf body, the culture device body comprises a box body, and a constant temperature control system is arranged in the box body; the culture shelf body comprises a plurality of placing plates, the placing plates are detachably mounted in the box body, a plurality of vent holes are formed in the placing plates, a plurality of connecting blocks are rotatably connected to the inner sides of the left side wall and the right side wall of the box body, sliding rails are arranged in the connecting blocks, and the sliding rails are arranged in the sliding rails. And sliding blocks are fixedly connected to the left end and the right end of the placement plate. The placing plate is rotatably mounted in the culture device, and when the culture dish needs to shake, the culture dish can be driven to shake through rotation of the placing plate, so that the shake of the culture dish does not need to be taken out from the culture device, and the culture dish is in a constant-temperature environment while being conveniently shaken; the culture of cartilage cells is facilitated.

The invention provides a composite biological scaffold based on chondrocyte microspheres as well as a preparation method and application thereof, and relates to the technical field of biomedical materials. The composite biological scaffold based on the cartilage cell microspheres is prepared by using a process flow combining a microfluidic technology and a biological printing technology, taking the cartilage cell microspheres as a high-activity construction unit and taking light-cured hydrogel as a support carrier. In-vitro experiments show that the scaffold significantly improves cell viability, extracellular matrix deposition and chondrogenesis gene expression level, and meanwhile, the scaffold is stable in form; in-vivo research shows that the scaffold is highly similar to natural auricular cartilage in the aspects of macroscopic morphology, histological characteristics and mechanical strength. The composite biological scaffold provided by the invention not only can effectively promote the regeneration of the elastic cartilage, but also makes up the key defects of the traditional hydrogel scaffold, and provides an innovative strategy with clinical application value for auricle defect repair.

The invention belongs to the technical field of biological medicine, and particularly discloses a hydrogel-state articular cartilage targeting nano drug delivery system and a preparation method thereof.The preparation method comprises the steps that G5PAMAM is modified with PEG, and a 50% PEGylated PAMAM material (PAMAM-PEG-Mal) is prepared; the preparation method comprises the following steps of: carrying synthesized miR-455-3p mimic by using the nano-carrier, and connecting cysteine terminated cartilage cell affinity peptide (CAP) and minimum autopeptide (MSP), so as to synthesize a cartilage targeting nano-carrier (CANC) with positive charges; a self-assembled neutral triblock hydrogel (PCEC) is used for packaging a cartilage targeting nano carrier with positive charges, and the hydrogel-state articular cartilage targeting nano drug delivery system with the solid content of 15% is obtained. Compared with the prior art, the hydrogel-state articular cartilage targeting nano drug delivery system has the advantages that the joint residence time of miR-455-3p mimic can be prolonged, the utilization efficiency of articular cartilage cells on miR-455-3p mimic can be improved, articular cartilage degeneration and OA progress can be effectively inhibited, and meanwhile, the hydrogel-state articular cartilage targeting nano drug delivery system has good biological safety.

The invention discloses mRNA (messenger Ribonucleic Acid) for coding FGF18 (Fibroblast Growth Factor 18) protein, an mRNA-LNP delivery system and application of the mRNA to preparation of a medicine for repairing cartilage and / or treating osteoarthritis, the mRNA comprises a 5 'UTR (Untranslated Region) sequence, an FGF18 coding sequence and a 3' UTR sequence which are sequentially connected, an amino acid sequence coded by the mRNA is shown as SEQ ID NO.7, the 5 'UTR sequence is shown as SEQ ID NO.3 or SEQ ID NO.4, and the 3' UTR sequence is shown as SEQ ID NO.5 or SEQ ID NO.6. The invention further discloses a preparation method of the mRNA. According to the invention, by designing and screening the UTR sequence and the coding region sequence, a plurality of mRNAs for coding the FGF18 protein, which have high stability and high translation efficiency in cartilage cells, are constructed; according to the present invention, the mRNA-LNPs is further adopted to wrap the mRNA, and the LNPs is optimized so as to finally obtain the mRNA-LNP delivery system capable of effectively infiltrating into the deep part of the cartilage and highly expressing the FGF18 protein, such that the effective treatment on the osteoarthritis is achieved.

The invention discloses a novel application of vitamin B12 in preparation of a medicine for preventing and / or treating osteoarthritis. Experimental research results show that the vitamin B12 can effectively relieve inflammation-induced oxidative stress and cartilage cell senescence by inhibiting an NF-kappa B signal channel, cartilage cell proliferation and matrix generation are promoted, and the vitamin B12 has a wide clinical application prospect in preparation of the medicine for preventing and / or treating osteoarthritis.

The invention discloses a preparation method of an ROS response microsphere encapsulated mitochondrial targeted nootkatone loaded Mxene multifunctional composite material, and relates to the technical field of biomedicine.The preparation method comprises the steps that carboxylated Mxene nanosheets are prepared and dissolved in dimethyl sulfoxide to obtain a product MXene-COOH-NK, then the product MXene-COOH-NK is dissolved in a 2-morpholine ethanesulfonic acid MES solution to obtain a product MXene-COOH-NK-Man, and the MXene-COOH-NK-Man is loaded on the Mxene multifunctional composite material to obtain the ROS response microsphere encapsulated mitochondrial targeted nootkatone loaded Mxene multifunctional composite material. On the basis, the ROS response microsphere encapsulated mitochondrial targeted Mxene loaded nootkatone is prepared. MXene has near-infrared photothermal conversion performance and supports light controlled drug release and local mild thermal therapy, MXene-COOH at NK can directly enrich chondrocyte mitochondria after being modified by mannosamine, expression of inflammatory factors is further inhibited, boric acid ester bonds are contained in the microspheres, and the MXene-COOH at NK can be used for preparing a drug for treating chondrocyte mitochondria, so that the MXene-COOH at NK can be used for preparing a drug for treating chondrocyte mitochondria. The nootkatone is broken under the high ROS environment condition of an OA focus, fixed-point and on-demand release of the medicine nootkatone is achieved, and whole body exposure is reduced.

This invention discloses a neutralizing nanobody targeting soluble interleukin-17 receptor D (sIL-17RD), its preparation method, and its applications. The heavy chain variable region (VHH) of this nanobody contains a heavy chain complementarity-determining region 3 (CDR-H3) that specifically binds to sIL-17RD, with a length of 10 amino acids and containing 2–6 lysine residues. This invention provides four nanobodies: CQ5, CQ13, CQ11, and CQ6, corresponding to well-defined CDR-H1, CDR-H2, and CDR-H3 sequence combinations. This nanobody can specifically bind to and neutralize the biological activity of sIL-17RD, reducing chondrocyte death, promoting proliferation, and maintaining extracellular matrix homeostasis by inhibiting the P2rx7-NLRP3 inflammasome-mediated chondrocyte pyroptosis pathway. This invention also protects the nucleic acid encoding this nanobody, the recombinant expression vector, the host cell, the pharmaceutical composition, and the preparation method. The nanobodies described can be used to prepare drugs for the prevention and treatment of inflammatory osteoarthritis and other osteoarthritis. They can inhibit synovitis, reverse cartilage and subchondral bone degeneration, and relieve pain, showing promising application prospects.

The application discloses a preparation method of ROS response microsphere encapsulated mitochondria targeted nocardicin loaded Mxene multifunctional composite material, relates to the technical field of biological medicine, and prepares carboxylated Mxene nanosheet, dissolves in dimethyl sulfoxide to obtain product MXene-COOH@NK, is dissolved in 2-morpholinoethanesulfonic acid MES solution to obtain product MXene-COOH@NK-Man, and on this basis, ROS response microsphere encapsulated mitochondria targeted Mxene loaded nocardicin is prepared. The ROS response microsphere encapsulated mitochondria targeted Mxene loaded nocardicin prepared by the application has the near-infrared light heat conversion performance of MXene to support light-controlled drug release and local mild thermotherapy, and after the modification of MXene-COOH@NK by mannose, the MXene-COOH@NK can be directly enriched with chondrocyte mitochondria, further inhibit the expression of inflammatory factors, the microsphere contains borate ester bonds, the borate ester bonds are broken under the high ROS environment condition of OA lesion, the site release of the drug nocardicin is realized, and the systemic exposure is reduced.

The invention discloses a medicine composition containing evodiamine, a medicine carrying system and application of the medicine carrying system in osteoarthritis, and belongs to the technical field of biological medicine. It is found for the first time that evodiamine can significantly inhibit cartilage cell apoptosis and promote cartilage cell proliferation; the brand new pharmacological activity provides a brand new candidate compound for the treatment of osteoarthritis. In order to further improve the curative effect, evodiamine and a medicine combination capable of intervening in macrophage polarization or adjusting the joint inflammation microenvironment are designed, the medicine combination can act on two key pathological links of cartilage repair and inflammation adjustment at the same time, and the treatment effect of osteoarthritis is greatly improved through the synergistic effect.