46 results about "Chondrocyte cell" patented technology

The invention provides a nanogel for rapid permeation of articular cartilage and responsive release of a growth factor, which is prepared by polymerizing a peptide fragment which can be cut by a substance (mainly enzyme) which can be specifically expressed on the surface of osteoarthritis cells or secreted into a matrix with the growth factor, an acrylamide monomer and a cationic micromolecule monomer. Unreacted monomer molecules are filtered out through an ultrafiltration tube or other filtering systems, so that the nanogel which can rapidly permeate into the deep part of articular cartilage and can be recognized and cut by enzyme highly expressed on the surface of osteoarthritis cells or secreted enzyme to release growth factors is prepared, and the effect of the growth factors can be more effectively exerted; the nanogel has strong cartilage infiltration capacity, can completely infiltrate cartilage with the thickness of 1mm within less than 3 days, can be cut by MT1-MMP protein specifically expressed on the surfaces of osteoarthritis cells, releases growth factors, is specifically combined with growth factor receptors on the surfaces of cartilage cells, and activates downstream signal channels, so that the proliferation of the cartilage cells is promoted, and the cartilage cell proliferation rate is increased. The cartilage matrix secretion is promoted, so that the cartilage matrix thickening effect is realized, and the osteoarthritis is prevented and treated.

The invention relates to the technical field of medicines, foods and health-care products, in particular to application of morusin in preparation of a product for treating osteoarthritis. A hydrogen peroxide induced chondrocyte in-vitro model and a D-galactose subcutaneous injection osteoarthritis animal model are used for screening and evaluating the morusin, and it is found that the morusin has the obvious effects of relieving articular cartilage injury, enhancing articular cartilage toughness, delaying the course of osteoarthritis and the like. Wide application prospects are realized in the aspect of preparing products for treating osteoarthritis.

The invention belongs to the technical field of biological medicines, and discloses a B cell and cartilage cell senescence targeting osteoarthritis treatment medicine. The invention provides an application of an anti-MIF antibody or an antigen binding fragment thereof as a B cell targeting immunomodulator in osteoarthritis treatment drugs, the anti-MIF antibody or the antigen binding fragment thereof regulates and controls an MIF / HMGB1 / MMP13 signal axis through a neutralization effect with MIF, so that the MIF and B cells are significantly co-localized, expression of HMGB1 in the B cells is promoted, and the osteoarthritis treatment effect is improved. The senescence of B cells is reduced and the B cells are promoted to regulate or repair phenotype transformation so as to destroy chronic inflammatory circulation and diffusion of pathogenic plasma cells, and the expression of MMP13 in joint tissues is reduced so as to maintain the steady state of cartilage tissues; the preparation method has an excellent application prospect in preparation of an OA intra-articular injection drug which realizes rapid anti-inflammatory and long-term cartilage protection effects and can realize a lasting curative effect through low-frequency drug delivery.

The invention belongs to the technical field of biomedicine, and particularly relates to a nucleic acid combination product for detecting SIAH2, a diagnostic kit for osteoarthritis and application of the diagnostic kit. According to the application, the models are established by using machine learning to analyze various cell death modes, and the application finds that in the models of the four cell death modes, the ferroptosis model has the highest diagnosis efficiency on the OA and identifies the corresponding biomarkers. It is found that SIAH2 is used as a biomarker to regulate occurrence and development of OA through cartilage cells, and a new diagnosis and treatment strategy is provided for OA.

The invention relates to the technical field of biological medicines, and particularly discloses application of emetine hydrochloride in preparation of a medicine for treating osteoarthritis. A cell test shows that the chondrocyte activity can be remarkably improved by an epecinol hydrochloride alkaline solution; animal model tests show that the epecinol hydrochloride alkali solution can maintain the steady state of chondrocyte metabolism, significantly reduce the cartilage injury degree of osteoarthritis mice and relieve osteoarthritis, and provides a new way for improving the osteoarthritis condition. The chemical structural formula of the emetine hydrochloride is shown in the specification.

The present invention relates to the technical field of biomedical materials, and specifically provides a bone and joint biological super-lubricating copolymer and a preparation method thereof. Dopamine is modified with methacrylic anhydride to prepare dopamine methacrylamide, and then a positively charged poly (2-ethyl-2-oxazoline) with good hydrophilicity and hydration lubrication performance and dopamine methacrylamide with adhesion performance are subjected to free radical polymerization to form a super-lubricating copolymer. The positively charged super-lubricating copolymer has good cell affinity, can promote the attachment, growth and differentiation of chondrocytes, and is beneficial to tissue repair and regeneration. The positively charged super-lubricating copolymer has high hydrophilicity, excellent hydration ability and lubricating properties, can adhere to the joint surface, absorb surrounding water molecules to form a hydration layer, effectively reduce surface friction and wear, and the unique adhesion properties of the copolymer and the phenol structure can effectively scavenge free radicals to achieve an anti-inflammatory effect.

The invention discloses a composition containing bovine bone collagen as well as a preparation method and application thereof, and belongs to the technical field of collagen application. The composition containing the bovine bone collagen is obtained by chemical crosslinking of bovine bone collagen magnesium chelate, sodium hyaluronate, chondroitin sulfate and bovine bone collagen through EDC / NHS. The composition containing bovine bone collagen is prepared from the following components in parts by weight: 5 to 10 parts of bovine bone collagen magnesium chelate, 2 to 5 parts of sodium hyaluronate, 0.5 to 2 parts of chondroitin sulfate and 20 to 30 parts of bovine bone collagen. The bovine bone collagen magnesium chelate, the sodium hyaluronate, the chondroitin sulfate and the bovine bone collagen in the composition containing the bovine bone collagen prepared by the invention have the characteristics of good biocompatibility, capability of promoting cell proliferation and differentiation into cartilage cells, and suitability for cartilage repair.

The invention relates to the technical field of biomedicine, and particularly discloses application of an exosome overexpressing a CAP gene and an LGR5 gene in preparation of a medicine for treating cartilage inflammation. The exosome for overexpressing the CAP gene and the LGR5 gene has targeting and cartilage protection functions, can be swallowed by cartilage cells and targeted to the cartilage cells, can improve the levels of a gene Col2a1 for cartilage generation and a gene Sox9 for regulating cartilage development, and can reduce the level of a gene Mmp13 for cartilage degradation, so that the exosome can be applied to cartilage protection of cartilage cells. The cartilage abrasion of osteoarthritis mice is relieved, and knee joints are protected, so that cartilage generation can be promoted, cartilage aging is relieved, and cartilage degradation is reduced in a complex inflammation environment. Furthermore, the invention provides the mesenchymal stem cell exocrine for over-expression of CAP / LGR5, a new technical scheme is provided for targeted therapy based on stem cell engineering, and the mesenchymal stem cell exocrine has a wide application prospect.

The present application relates to lipid vacuole (aka LV)-containing lipocartilage cells (aka LCs), and methods for the differentiation of human lipocartilage progenitors into LV-containing LCs. Also disclosed are methods related to purifying, labeling, maturing, and delivering of LV-containing LCs to a subject in need thereof.

The invention belongs to the technical field of biomedical materials, and particularly relates to a triple-helix self-assembly recombinant humanized I-type collagen fiber implant as well as a preparation method and application of the triple-helix self-assembly recombinant humanized I-type collagen fiber implant. The invention firstly provides a recombinant humanized type I collagen, wherein the recombinant humanized type I collagen has a unique triple helix structure and an excellent self-assembly property; secondly, by optimizing the self-assembly process of the recombinant humanized I-type collagen, the recombinant humanized I-type collagen implant with orderly arranged collagen fibers is prepared, and the implant has the advantages of excellent injectability, durability, high biological safety, no heating and hemolysis risk, low immunogenicity, good biocompatibility, good biocompatibility and good biocompatibility. Adhesion, migration, proliferation and differentiation of human skin fibroblasts are remarkably promoted, adhesion, migration and proliferation of cartilage cells and chondrogenic differentiation of bone marrow mesenchymal stem cells can be remarkably promoted, and the application to the fields of skin, cartilage, bone and the like can be achieved.

It is an object of the present invention a method to obtain a cartilage cell population enriched in cartilage progenitor cells (CPCs), said method comprising culturing said cells in culture at a density between 10 and 500 cells / cm2. In a further aspect, it is here claimed a population of cartilage cells enriched in CPCs obtainable by means of said method, and their use in the treatment of chondral lesions or joint degeneration.

The invention relates to a method for evaluating the biological value of mesenchymal stem cells, and belongs to the technical field of biology. The method comprises the following steps: firstly, respectively collecting conditioned culture media of mesenchymal stem cells from different donor sources and mesenchymal stem cells expressed by silence TSP2, and detecting the concentration of TSP2 in the culture media by adopting an enzyme-linked immunosorbent assay method; furthermore, inflammatory factors are used for inducing cartilage cells, a conditioned culture medium is replaced for culture for 24 h, then the cells are collected, messenger RNA (mRNA) expression change of II-type collagen of the cells is detected, and the correlation between the TSP2 concentration of the conditioned culture medium and the mRNA expression abundance of the II-type collagen of the cartilage cells is established; the TSP2 concentration in the mesenchymal stem cell culture supernatant is measured, the TSP2 concentration can be used for evaluating the biological value of the mesenchymal stem cells in cartilage regeneration and protection in treatment of osteoarthritis and other degenerative diseases, and a reliable basis is provided for standardization and quality control of mesenchymal stem cell treatment.

The invention provides an injectable magnesium-loaded lipid-based super-lubricating hydrogel microsphere, an articular cavity injection preparation and a preparation method of the injectable magnesium-loaded lipid-based super-lubricating hydrogel microsphere. The method comprises the following steps: S1, preparing a magnesium-loaded multilayer vesicular liposome suspension containing magnesium ions; s2, preparing a non-crosslinked magnesium-containing hydrogel; and S3, mixing the magnesium-loaded multilayer vesicular liposome suspension and the magnesium-containing hydrogel to prepare hydrogel liquid drops, and carrying out crosslinking to form the magnesium-loaded lipid-based super-lubricating hydrogel microspheres. The magnesium loading amount of the lipid-based super-lubrication hydrogel microspheres can be greatly improved; on one hand, the biological function provided by magnesium ions can be greatly prolonged, that is, repairing of cartilage by synovial fluid progenitor cells is continuously promoted; on the other hand, the self-lubricating effect of the lipid-based super-lubricating hydrogel microspheres can reduce the secondary wear of the new cartilage; in addition, magnesium ions can remarkably promote expression of cartilage cells PRG4, so that the stable state of the cartilage cells can be maintained, and joint lubrication can be further enhanced.

1. The name of the design product: bone magnetic growth instrument (MT). 2. The use of the design product: for promoting the regeneration and acceleration of chondrocytes by stimulating epiphyseal cells, and subsequent calcification into hard bone to achieve related auxiliary treatment of bone growth. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

The invention discloses an antler plate oligopeptide as well as a preparation method and application thereof, and relates to the technical field of bioactive peptides. The preparation method comprises the following steps: extracting an antler plate protein crude extract by taking antler plates as a raw material, preparing short peptides with antler plate molecular weight of less than 3k through pepsin and trypsin hydrolysis, and performing Sephadex G-15 gel chromatography to obtain the antler plate short peptides. The prepared antler plate oligopeptide is low in molecular weight and high in hydrophobicity, easily penetrates through a biological membrane barrier to reach an in-vivo effective part, fully plays the biological activity effect of small molecule peptide, can effectively promote C28 / I2 cartilage cell proliferation, can increase the expression of osteoarthritis related phenotype protein Collagen II in an in-vitro model, can reduce the expression of MMP3, MMP13 and ADAMTS-5, and can be used for treating osteoarthritis. Meanwhile, cartilage matrix degradation in an osteoarthritis rat model is inhibited, cartilage injury is improved, and inflammatory response induced by osteoarthritis is reduced.

The utility model discloses an autologous chondrocyte culture device for cartilage tissue repair, which comprises a culture device body and a culture shelf body, the culture device body comprises a box body, and a constant temperature control system is arranged in the box body; the culture shelf body comprises a plurality of placing plates, the placing plates are detachably mounted in the box body, a plurality of vent holes are formed in the placing plates, a plurality of connecting blocks are rotatably connected to the inner sides of the left side wall and the right side wall of the box body, sliding rails are arranged in the connecting blocks, and the sliding rails are arranged in the sliding rails. And sliding blocks are fixedly connected to the left end and the right end of the placement plate. The placing plate is rotatably mounted in the culture device, and when the culture dish needs to shake, the culture dish can be driven to shake through rotation of the placing plate, so that the shake of the culture dish does not need to be taken out from the culture device, and the culture dish is in a constant-temperature environment while being conveniently shaken; the culture of cartilage cells is facilitated.

The invention provides a composite biological scaffold based on chondrocyte microspheres as well as a preparation method and application thereof, and relates to the technical field of biomedical materials. The composite biological scaffold based on the cartilage cell microspheres is prepared by using a process flow combining a microfluidic technology and a biological printing technology, taking the cartilage cell microspheres as a high-activity construction unit and taking light-cured hydrogel as a support carrier. In-vitro experiments show that the scaffold significantly improves cell viability, extracellular matrix deposition and chondrogenesis gene expression level, and meanwhile, the scaffold is stable in form; in-vivo research shows that the scaffold is highly similar to natural auricular cartilage in the aspects of macroscopic morphology, histological characteristics and mechanical strength. The composite biological scaffold provided by the invention not only can effectively promote the regeneration of the elastic cartilage, but also makes up the key defects of the traditional hydrogel scaffold, and provides an innovative strategy with clinical application value for auricle defect repair.

The invention discloses a novel application of vitamin B12 in preparation of a medicine for preventing and / or treating osteoarthritis. Experimental research results show that the vitamin B12 can effectively relieve inflammation-induced oxidative stress and cartilage cell senescence by inhibiting an NF-kappa B signal channel, cartilage cell proliferation and matrix generation are promoted, and the vitamin B12 has a wide clinical application prospect in preparation of the medicine for preventing and / or treating osteoarthritis.

The invention discloses a preparation method of an ROS response microsphere encapsulated mitochondrial targeted nootkatone loaded Mxene multifunctional composite material, and relates to the technical field of biomedicine.The preparation method comprises the steps that carboxylated Mxene nanosheets are prepared and dissolved in dimethyl sulfoxide to obtain a product MXene-COOH-NK, then the product MXene-COOH-NK is dissolved in a 2-morpholine ethanesulfonic acid MES solution to obtain a product MXene-COOH-NK-Man, and the MXene-COOH-NK-Man is loaded on the Mxene multifunctional composite material to obtain the ROS response microsphere encapsulated mitochondrial targeted nootkatone loaded Mxene multifunctional composite material. On the basis, the ROS response microsphere encapsulated mitochondrial targeted Mxene loaded nootkatone is prepared. MXene has near-infrared photothermal conversion performance and supports light controlled drug release and local mild thermal therapy, MXene-COOH at NK can directly enrich chondrocyte mitochondria after being modified by mannosamine, expression of inflammatory factors is further inhibited, boric acid ester bonds are contained in the microspheres, and the MXene-COOH at NK can be used for preparing a drug for treating chondrocyte mitochondria, so that the MXene-COOH at NK can be used for preparing a drug for treating chondrocyte mitochondria. The nootkatone is broken under the high ROS environment condition of an OA focus, fixed-point and on-demand release of the medicine nootkatone is achieved, and whole body exposure is reduced.

This invention discloses a neutralizing nanobody targeting soluble interleukin-17 receptor D (sIL-17RD), its preparation method, and its applications. The heavy chain variable region (VHH) of this nanobody contains a heavy chain complementarity-determining region 3 (CDR-H3) that specifically binds to sIL-17RD, with a length of 10 amino acids and containing 2–6 lysine residues. This invention provides four nanobodies: CQ5, CQ13, CQ11, and CQ6, corresponding to well-defined CDR-H1, CDR-H2, and CDR-H3 sequence combinations. This nanobody can specifically bind to and neutralize the biological activity of sIL-17RD, reducing chondrocyte death, promoting proliferation, and maintaining extracellular matrix homeostasis by inhibiting the P2rx7-NLRP3 inflammasome-mediated chondrocyte pyroptosis pathway. This invention also protects the nucleic acid encoding this nanobody, the recombinant expression vector, the host cell, the pharmaceutical composition, and the preparation method. The nanobodies described can be used to prepare drugs for the prevention and treatment of inflammatory osteoarthritis and other osteoarthritis. They can inhibit synovitis, reverse cartilage and subchondral bone degeneration, and relieve pain, showing promising application prospects.

The application discloses a preparation method of ROS response microsphere encapsulated mitochondria targeted nocardicin loaded Mxene multifunctional composite material, relates to the technical field of biological medicine, and prepares carboxylated Mxene nanosheet, dissolves in dimethyl sulfoxide to obtain product MXene-COOH@NK, is dissolved in 2-morpholinoethanesulfonic acid MES solution to obtain product MXene-COOH@NK-Man, and on this basis, ROS response microsphere encapsulated mitochondria targeted Mxene loaded nocardicin is prepared. The ROS response microsphere encapsulated mitochondria targeted Mxene loaded nocardicin prepared by the application has the near-infrared light heat conversion performance of MXene to support light-controlled drug release and local mild thermotherapy, and after the modification of MXene-COOH@NK by mannose, the MXene-COOH@NK can be directly enriched with chondrocyte mitochondria, further inhibit the expression of inflammatory factors, the microsphere contains borate ester bonds, the borate ester bonds are broken under the high ROS environment condition of OA lesion, the site release of the drug nocardicin is realized, and the systemic exposure is reduced.

The invention discloses a medicine composition containing evodiamine, a medicine carrying system and application of the medicine carrying system in osteoarthritis, and belongs to the technical field of biological medicine. It is found for the first time that evodiamine can significantly inhibit cartilage cell apoptosis and promote cartilage cell proliferation; the brand new pharmacological activity provides a brand new candidate compound for the treatment of osteoarthritis. In order to further improve the curative effect, evodiamine and a medicine combination capable of intervening in macrophage polarization or adjusting the joint inflammation microenvironment are designed, the medicine combination can act on two key pathological links of cartilage repair and inflammation adjustment at the same time, and the treatment effect of osteoarthritis is greatly improved through the synergistic effect.

This invention discloses a highly sensitive chondrocyte cytoplasmic viscosity probe and its preparation method, belonging to the field of cell probe technology. The method includes: drawing a borosilicate capillary into a nanoparticle with an inner diameter of 70-490 nm; filling the tube lumen with an aminosilane solution and incubating at room temperature to achieve amino functionalization modification; and finally injecting PBS buffer to obtain the probe. Amino modification significantly enhances the charge density of the nanoparticle's inner wall. When the inner diameter of the nanoparticle tip is reduced to 120 nm, the spatial confinement effect significantly enhances the regulatory effect of the enhanced surface charge on ion transport, strengthens the electric double layer effect and electroosmotic effect, and makes the ion current highly sensitive to changes in solution viscosity, thereby greatly improving the probe's response sensitivity to small viscosity fluctuations. The preparation method of this invention is simple and efficient, and the resulting probe has a fast response speed, enabling real-time dynamic monitoring of chondrocyte cytoplasmic viscosity, providing a highly sensitive tool for early warning and mechanism research of diseases such as osteoarthritis.

This application relates to stem cell biology and regenerative medicine. Disclosed herein are agents and methods for converting non-chondrocyte cells to chondrocytes or chondrogenic ceils, related cells, related compositions, related products, and related uses.

The invention relates to the technical field of traditional Chinese medicines, in particular to a medicinal and edible traditional Chinese medicine compound GCNY-0318 for treating osteoarthritis and a preparation method of tablets of the medicinal and edible traditional Chinese medicine compound GCNY-0318. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 10-30 parts of liquorice, 5-15 parts of ginseng, 3-10 parts of dried ginger, 10-20 parts of dandelion, 5-15 parts of momordica grosvenori, 10-20 parts of radix polygonati officinalis and 5-15 parts of Chinese dates. The invention provides a therapy for delaying senescence of cartilage cells of osteoarthritis patients and regulating inflammation, the activation of a YWHAZ / PI3K / AKT signal channel is inhibited, and the integrity of articular cartilage of osteoarthritis is protected, so that the pathological progress of osteoarthritis is slowed down.

The invention relates to a filler composition for cartilage cell differentiation capacity and collagen activity and a preparation method thereof. The composition comprises a mixture of PLGA microparticles and HA and PDRN microparticles, and can be usefully used as a filler composition by stably inducing cell adsorption, improving collagen production, activating cartilage cell regeneration, and verifying tissue repair effects.

Provided herein are systems for regulating gene expression, methods for their use, and cells, such as cells engineered for the purpose of differentiating chondroprogenitor cells.

The invention relates to the technical field of medicine, and discloses hyaluronic acid-silk fibroin hydrogel injected into a bone joint cavity and a preparation method of the hyaluronic acid-silk fibroin hydrogel. The gel particles contain hyaluronic acid or salt thereof, silk fibroin and a cross-linking agent; the cross-linking agent is BDDE; the molecular weight of the silk fibroin is 2.5-100 thousand Daltons; the preparation method comprises the following steps: a, dissolving a certain amount of hyaluronic acid or salt thereof and silk fibroin powder in an alkaline solution, uniformly stirring, and carrying out defoaming treatment; b, adding a proper amount of cross-linking agent BDDE into the solution defoamed in the step a to form a mixed solution, carrying out cross-linking, and then dialyzing; c, the gel obtained after dialysis in the step b is sieved through a 40-100-mesh sieve to be granulated, and gel particles are obtained; and d, adding a proper amount of aqueous medium into the gel particles in the step c for resuspension to obtain the hyaluronic acid-silk fibroin hydrogel. The hydrogel disclosed by the invention has excellent mechanical properties, viscoelasticity and abundant porous structures, is remarkable in anti-inflammatory effect, can promote the growth of cartilage cells, is used for intraarticular injection and treatment of osteoarticular related diseases, and has a better industrial production prospect.

The invention belongs to the technical field of biological medicines, and particularly relates to application of purine deoxynucleotide in preparation of a medicine for treating osteoarthritis, the purine deoxynucleotide comprises any one of deoxyguanosine monophosphate and deoxyadenosine monophosphate, the molecular formula of the deoxyguanosine monophosphate is C10H12N5Na2O7P, and the molecular formula of the deoxyadenosine monophosphate is C10H12N5Na2O7P. The molecular formula of the deoxyadenosine monophosphate is C10H14N5O6P. A research result shows that the purine deoxynucleotide has an inhibiting effect on the activity of cartilage cells; animal model tests show that the purine deoxynucleotide can significantly reduce the cartilage injury degree of osteoarthritis mice and relieve osteoarthritis, and a new way is provided for improvement of osteoarthritis conditions.