Effect of copper wire stem root in preparation of medicine for treating osteoarthritis

A technology for treating osteoarthritis and medicines, applied in the field of copper vine roots in the preparation of medicines for treating osteoarthritis, can solve the problems of no copper vine root osteoarthritis, few copper vine roots, etc., and achieve The effect of improving the survival rate of chondrocytes

Pending Publication Date: 2022-07-15
丽水市中医院
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are few studies on the chemical components and pharmacological effects of cop

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Effect of copper wire stem root in preparation of medicine for treating osteoarthritis
  • Effect of copper wire stem root in preparation of medicine for treating osteoarthritis
  • Effect of copper wire stem root in preparation of medicine for treating osteoarthritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1. Extraction and separation

[0038] Take 10kg of the medicinal material of the root of copper wire rattan, crush it, and extract it three times with 6 times the amount of 80% ethanol by refluxing for 2 hours each time. The extract is concentrated under reduced pressure. The volume ratio was 1:1 and extracted successively, and the extracts were concentrated respectively to obtain petroleum ether extraction part (51.17g), ethyl acetate extraction part (237.75g) and n-butanol extraction part (199.12g).

[0039] The ethyl acetate extracted part was subjected to silica gel column chromatography Sephadex LH-20 column chromatography, eluted with petroleum ether-ethyl acetate gradient, and detected by thin layer, combined and concentrated to obtain a total of 11 fractions of Fr.1-11.

[0040] Among them, fraction Fr.2 was prepared by liquid phase (23% acetonitrile, 0.1% acetic acid) to obtain compound 1 (21.5 mg).

[0041]Fractions Fr.3 and 4 were combined, and then subjecte...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an effect of copper wire stem root in preparation of a medicine for treating osteoarthritis. According to the invention, chemical components contained in the copper wire stem root are separated and represented, and it is found for the first time that the copper wire stem root contains various anti-osteoarthritis active components, including 6-hydroxy-2-isopropyl-7-methyl-1, 4-naphthoquinone, protocatechualdehyde, (E)-3, 3, 4, 5, 6-tetramethyl-1, 3, 4, 5-tetramethyl-1, 3, 5-tetramethyl-1, 3, 5-tetramethyl-1, 3, 5-tetramethyl-1, 3, the compound is prepared from 2, 4-dihydroxybenzylidene acetone, 3-chloro-4-hydroxy-benzoic acid, kaempferol-3-O-rutinoside, p-hydroxymethylene benzyl acetone and the like. According to the application disclosed by the invention, the (E)-3, 4-dihydroxybenzylidene acetone, the 3-chloro-4-hydroxy-benzoic acid and the p-hydroxymethylene benzyl acetone are found for the first time, so that not only can the content of MMP-13 in a cartilage cell culture supernatant be remarkably reduced, but also the survival rate of the cartilage cells after IL-1beta intervention can be remarkably improved; therefore, the copper wire stem root and the three compounds can be used for preparing the medicine for treating osteoarthritis.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and particularly relates to the effect of copper wire rattan root in the preparation of a drug for treating osteoarthritis. Background technique [0002] The main manifestation of osteoarthritis is the inflammatory reaction of the body or the bones and joints. At present, there is no cure for arthritis. Most of them use non-steroidal anti-inflammatory drugs or glucocorticoids to alleviate the symptoms. The treatment of such diseases needs to be further developed. development. [0003] Copper wire rattan root is a traditional medicinal material of the She nationality. It is the dry root and rhizome of Lygodium japonicum (Thunb.) Sw., which is now included in the 2015 edition of "Zhejiang Province Traditional Chinese Medicine Processing Specifications", also known as iron centipede , wire grass, iron footed centipede root, sea sand root. Its properties are sweet, light, cold, non-toxic, and h...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K36/11A61K31/12A61K31/192A61K31/05A61P19/02A61P19/00
CPCA61K36/11A61K31/12A61K31/192A61K31/05A61P19/02A61P19/00
Inventor 倪京丽张晓芹张尊敬陈海涛时烨王慧玉毛佳乐刘爽
Owner 丽水市中医院
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products