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Zuogui pill nanoparticle drug delivery system and preparation method thereof

A technology of nano-microspheres and Zuogui Pills, which is applied in the direction of pharmaceutical formulas, medical preparations with no active ingredients, medical preparations containing active ingredients, etc., which can solve the problem of poor biofilm permeability and Zuogui Pills that do not have sustained release Effect, poor stability and other problems, to achieve the effect of promoting proliferation

Inactive Publication Date: 2015-05-20
HANGZHOU CITY XIAOSHAN DISTRICT TRADITIONAL CHINESE MEDICAL HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, commonly used cytokines in cartilage tissue engineering research include: IGF-I, TGF, BMP, etc., but there are many shortcomings: expensive, poor stability, short half-life in vivo, poor biofilm permeability, and in the free state highly susceptible to inactivation, thereby limiting its effectiveness
[0010] The Zuogui Pill prepared by the above method does not have a sustained release effect, and has no advantages in terms of sustainability and controllability.

Method used

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  • Zuogui pill nanoparticle drug delivery system and preparation method thereof
  • Zuogui pill nanoparticle drug delivery system and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Preparation of Zuogui Pill Dry Powder

[0033] According to the dosage ratio formula of "Jingyue Quanshu": rehmannia glutinosa 24 g, yam 12 g, medlar 12 g, cornus 12 g, achyranthes bidentata 9 g, dodder 12 g, tortoise shell gum 12 g, antler gum 12 g, total crude drug The amount is 105 grams. Place in a 500ml beaker, add 150 ml of deionized water, soak for 4 hours, boil and then fry for 0.5 hours, filter, add 50 ml of distilled water for the second time, boil for 0.5 hours and filter, mix the filtrate twice, concentrate to about 30 ml, transfer to In a 50ml mouthless tall beaker, the electric heating plate was slowly heated to dryness to obtain the dry powder of Zuoguiwan, which was sealed at 4°C for later use.

Embodiment 2

[0034] The preparation of embodiment 2 Zuoguiwan poly(n-butyl cyanoacrylate) nano-microspheres

[0035] Take 20 mg of Zuoguiwan dry powder, dissolve it in 10 ml of 2.0 mol / L guanidine hydrochloride solution, and slowly add it into the solution containing 0.4% Tween-80 and 0.02% sodium pyrosulfite in an ice bath at 0-5°C under magnetic stirring. In 20 ml of double-distilled water, adjust the pH value of the solution to 3.5 with 1.0 mol / L HCl, add 2.4 mL of n-butyl cyanoacrylate monomer dropwise under constant stirring, and continue stirring for 40 min to obtain a milky white colloidal suspension liquid. Dialyze with double distilled water for 48 h at 4°C, filter through a 0.45 μm membrane, freeze-dry in vacuum to make lyophilized powder, and store in a sealed container at 4°C.

Embodiment 3

[0036] Example 3 The preparation of Zuoguiwan poly(n-butyl cyanoacrylate) nano-microspheres

[0037]Take 2 mg of Zuogui Wan dry powder, dissolve it in 10 ml 2.0 mol / L guanidine hydrochloride solution, and slowly add it into the solution containing 0.4% Tween-80 and 0.02% sodium metabisulfite in an ice bath at 0-5°C under magnetic stirring. In 20 ml of double-distilled water, adjust the pH value of the solution to 3.5 with 1.0 mol / L HCl, add 2.4 mL of n-butyl cyanoacrylate monomer dropwise under constant stirring, and continue stirring for 40 min to obtain a milky white colloidal suspension liquid. Dialyze with double distilled water for 48 h at 4°C, filter through a 0.45 μm membrane, freeze-dry in vacuum to make lyophilized powder, and store in a sealed container at 4°C.

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Abstract

The invention relates to a zuogui pill nanoparticle drug delivery system and a preparation method thereof. The zuogui pill nanoparticle drug delivery system comprises a zuogui pill extract and a polybutylcyanoacrylate nano carrier, wherein the mass ratio of the zuogui pill extract to the polybutylcyanoacrylate nano carrier is 1 to (0.1-10). Due to the technical scheme, with polybutylcyanoacrylate as a raw material, the zuogui pill nanoparticle drug delivery system is prepared by an improved emulsion polymerization method. The zuogui pill nanoparticle drug delivery system used by people has the advantages of continuity, high efficiency, controllability and the like, is capable of continuously and efficiently releasing the zuogui pill effective components, promoting chondrocyte proliferation and differentiation, and is natural, free of toxicity and high in efficiency.

Description

technical field [0001] The invention relates to a sustained-release system of Zuogui pill nano microspheres and a preparation method thereof. Background technique [0002] At present, surgical treatment is mainly used for articular cartilage injury clinically, including: arthroscopic repair, microfracture, subchondral bone drilling, autologous cartilage transplantation and autologous chondrocyte transplantation. However, the clinical curative effect of these treatment methods is not satisfactory, and there are many shortcomings. Among them, the short-term curative effect of arthroscopic repair is obvious, but it can only delay the process of cartilage wear, but cannot promote the repair of cartilage defects, and cannot ultimately prevent the occurrence of arthritis. Microfracture and subchondral bone drilling aim to infiltrate the stem cells in the marrow cavity of the subchondral bone into the cartilage injury site and initiate the cartilage repair mechanism. However, due...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8945A61K9/16A61K47/32A61P19/08A61K35/32A61K35/586
Inventor 黄忠名李俊华汤样华胡文跃
Owner HANGZHOU CITY XIAOSHAN DISTRICT TRADITIONAL CHINESE MEDICAL HOSPITAL
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