Culture dish for chondrocyte cell sheets

Abstract

The invention discloses a culture dish for chondrocyte cell sheets. The culture dish comprises a cell culture dish container body made of polystyrene, an upper end cover made of polystyrene is arranged on the cell culture dish container body, an arc-shaped cell suspension adding opening is formed in the side wall of the cell culture dish container body, a sealing end cover is arranged on the arc-shaped cell suspension adding opening, and a polycarbonate semipermeable membrane capable of filtering molecules with 5Kb molecular weight is arranged in the middle of the interior of the cell culturedish container body. The polycarbonate semipermeable membrane provided by the invention allows free passage of metabolic waste and nutrient components, and does not allow passage of a factor corresponding to the chondrogenesis promotion. During liquid change every time, only the liquid above the semipermeable membrane is changed, the chondrogenesis promotion factor does not need to be additionallyadded, the autocrine factor is reserved, and the production cost is reduced. The product can contain more liquid, the operation of changing the liquid every day is avoided, and the pollution possibility and the heavy work are reduced. Meanwhile, the culture dish is reasonable and simple in structural design, easy to implement and suitable for application and popularization.

Description

technical field [0001] The invention relates to the technical field of chondrocyte culture vessels, in particular to a chondrocyte membrane culture vessel. Background technique [0002] Articular cartilage is mainly avascular tissue composed of chondrocytes and extracellular matrix. It mainly relies on joint movement and extrusion to absorb nutrients. Therefore, the self-repair ability of cartilage damage is relatively weak. Clinical research has limited patient tissue materials, and also because Chondrocytes are terminally differentiated cells with limited proliferation ability in vitro and are prone to dedifferentiation. Dedifferentiation refers to the process in which differentiated cells lose their unique structure and function and become undifferentiated cells. Therefore, improving and maintaining chondrocyte phenotype and promoting chondrocyte chondrification play a pivotal role in the clinical application of chondrocytes. [0003] In the prior art, high-density chond...

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Problems solved by technology

[0005] The purpose of the present invention is to address the above problems, to provide a chondrocyte membrane culture dish, which can reduce the high cost of the chondrogenic induction process and reduce the pollution that may be caused by frequent liquid replacement through a simple structural design and heavy workload issues

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