Application of atler stem cell exosome in preparation of products for improving or treating osteoarthritis and delaying cell senescence

An osteoarthritis and exosome technology, applied in the field of biomedicine, can solve the problems of limiting the application of cell transplantation, low survival rate and survival time, etc., and achieve the effect of wide application prospect, reducing cartilage damage and stable treatment effect.

Active Publication Date: 2021-08-03
INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the low post-transplantation survival rate and survival time severely limit the clinical application of cell transplantation

Method used

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  • Application of atler stem cell exosome in preparation of products for improving or treating osteoarthritis and delaying cell senescence
  • Application of atler stem cell exosome in preparation of products for improving or treating osteoarthritis and delaying cell senescence
  • Application of atler stem cell exosome in preparation of products for improving or treating osteoarthritis and delaying cell senescence

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Example 1 Extraction and identification of velvet antler stem cell exosomes

[0067] 1. Antler stem cell culture

[0068] Antler stem cells isolated from sika deer antler were placed in a petri dish at 37°C, 5% CO 2 The culture medium was incubated in an incubator, and the medium used was MSC medium, and the components included MEMα basal medium (Thermo Fisher Scientific), 10% fetal bovine serum (Thermo Fisher Scientific), 1% penicillin / streptomycin (Thermo Fisher Scientific), 0.1 mM NEAAs (MSC Thermo Fisher Scientific), 1ng / mL FGF2 (JointProtein Central. Fetal bovine serum needs to be centrifuged at 100,000×g for 16 hours at 4°C to remove exosomes before preparing the medium, and the supernatant is taken for later use.

[0069] 2. Extraction of exosomes from velvet antler stem cells

[0070] When the antler stem cells grew to 80% confluence, the fresh medium was replaced, and the conditioned medium was collected for exosome purification after continuing to culture fo...

Embodiment 2

[0073] Example 2 Antler stem cell exosomes delay the process of replicative aging of human mesenchymal stem cells

[0074] 1. Detection of monoclonal formation ability

[0075] The late-generation (P12) human-derived mesenchymal stem cells hMSC (derived from embryonic stem cells) and the late-generation (P12) human-derived mesenchymal stem cells hPMSC (derived from tissue) were selected for the detection experiment of monoclonal formation ability. hMSCs and hPMSCs were seeded in collagen-precoated 12-well culture plates at a density of 3000 per well, respectively. A phosphate buffer treatment group and an exosome treatment group were set up to treat the cells, with 3 wells in each group, and the medium was changed and treated every other day. Phosphate buffered saline treatment group (Veh): Cells were incubated at 37° C. in 5% CO in MEMα+PBS (liquid medium obtained by adding 10 microliters of phosphate buffered saline to 1 ml of MEMα basal medium in Example 1) 2 Cultured und...

Embodiment 3

[0098] Example 3 Therapeutic Effect of Antler Stem Cell Exosomes on Osteoarthritis

[0099] The mouse osteoarthritis model was established with C57BL / 6J mice according to the literature method. Build method: "XiaoqingRen, Boqiang Hu, Moshi Song, Zhichao Ding, Yujiao Dang, Zunpeng Liu, Weiqi Zhang, Qianzhao Ji, Ruotong Ren, Jianjian Ding, Piu Chan, Changtao Jiang, Keqiong Ye, JingQu, Fuchou Tang, Guang-Hui Liu.Maintenance of Nuclear Homeostasis by CBX4Alleviates Senescence and Osteoarthritis.Cell Rep.2019;26(13):3643-3656.”

[0100] Osteoarthritis model mice weighing 20-25 g and 8 weeks old were randomly divided into three groups, Sham group, OA+Veh group and OA+Exo group, with 15 mice in each group. Sham group was the sham operation control group.

[0101] One week after operation, the OA+Veh group and OA+Exo group began to inject phosphate buffer saline or exosomes into the joint cavity for intervention, and the intervention interval was once a week for 8 weeks. After 8 we...

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Abstract

The invention discloses application of atler stem cell exosome in preparation of products for improving or treating osteoarthritis and delaying cell senescence. The invention particularly discloses application of a velvet antler-derived stem cell exosome in preparation of products for preventing, improving or treating individual osteoarthritis and application thereof in preparation of products for delaying cell senescence. The cell exosome has a stable treatment effect, and can inhibit or reduce the expression of inflammatory factors; the grip strength of arthritis mice is enhanced, and the motion function is recovered; the bone density of a joint area is enhanced, so that the mechanical capacity of a skeleton structure is enhanced, and the symptom of osteoarthritis is improved; cartilage injury in the joint area is relieved; the growth and differentiation of cartilage cells are effectively promoted, and the damage and injury of soft tissues are relieved; the composition can effectively improve and treat osteoarthritis, has the effect of delaying senescence of human mesenchymal stem cells, and has wide application prospects in the fields of osteoarthritis treatment and cosmetics.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of antler stem cell exosomes in the preparation of products for improving or treating osteoarthritis and delaying cell aging. Background technique [0002] Stem cell depletion is one of the main drivers of aging in the body, leading to stem cell transplantation as a way to treat age-related diseases. Osteoarthritis (OA) is a common aging-related disease, and its incidence gradually increases with age. Along with aging, various cells in the joints, such as chondrocytes, synoviocytes, and mesenchymal stem cells, undergo cellular senescence and functional degeneration. Among them, the aging of mesenchymal stem cells is considered to be one of the important causes of osteoarthritis. Mesenchymal stem cells (MSCs) are adult stem cells with multiple differentiation potentials that exist in various tissues and organs. Because of their good immune regulation ability and low tu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K35/32A61P19/02A61P29/00A61P17/02A61P39/00A61K8/99A61Q19/08
CPCA61K35/28A61K35/32A61P19/02A61P29/00A61K8/99A61Q19/08
Inventor 刘光慧曲静张维绮王思雷敬辉蒋晓钰李维
Owner INST OF ZOOLOGY CHINESE ACAD OF SCI
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