Micro-fluidic chip-based bionic in-vivo articular cartilage microenvironment structure

Abstract

The invention discloses a micro-fluidic chip-based bionic in-vivo articular cartilage microenvironment structure, and relates to the technical field of microfluidics, the micro-fluidic chip-based bionic in-vivo articular cartilage microenvironment structure comprises an upper layer, a middle layer and a lower layer which are stacked in sequence, the upper layer and the lower layer are both provided with pressure systems, and the middle layer is provided with a concentration gradient generator and a plurality of stem cell culture units. The concentration gradient generator is communicated with the cell culture unit, and the pressure system corresponds to the cell culture unit. According to the method, a physical microenvironment and a chemical microenvironment are integrated, high-throughput screening can be carried out on a cartilage cell in-vitro amplification environment by utilizing a small amount of cells, and the optimal factor concentration and compressive stress stimulation intensity suitable for cartilage cell proliferation and maintaining phenotypes are selected in an optimized manner; the structure proves the feasibility of the micro-fluidic chip technology in cartilage tissue engineering application, simulates the in-vivo mechanical stimulation microenvironment, and provides a scheme for in-vitro rapid amplification of cartilage cells, thereby improving the quality of seed cells and improving the autologous cartilage cell transplantation treatment effect.

Description

technical field [0001] The invention relates to the field of microfluidic technology, in particular to a microfluidic chip-based bionic articular cartilage microenvironment structure in vivo. Background technique [0002] Articular cartilage has no blood supply, lymphatic drainage and innervation, and has extremely weak self-repair ability. Under physiological conditions in vivo, articular cartilage is mainly stimulated by physical microenvironmental stimuli such as compressive stress and interstitial fluid flow, and chemical microenvironmental stimuli such as synovial fluid lubrication and penetration of nutrients. Therefore, external microenvironmental signal stimulation plays an important role in the metabolism of articular chondrocytes and maintaining the stability of the internal environment of cartilage matrix. For healthy articular cartilage, about 70-80% of the composition is water, and the metabolism and nutrient delivery of chondrocytes are mainly regulated by the...

AI Technical Summary

Problems solved by technology

However, this device uses a simple single pressure microenvironment, cell-mixed hydrogel (polyethylene glycol diacrylate (PEGDA)) material, which cannot better simulate the gap generated by the dynamic compression of the cartilage extracellular matrix material. Matrix material and structural microenvironment for sexual fluid flow and chondrocyte growth

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