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Target lncRNA: MSTRG.27033.1 for learning and memory dysfunction caused by microwave radiation

A technology of MSTRG.27033.1, microwave radiation, applied in DNA/RNA fragments, recombinant DNA technology, determination/inspection of microorganisms, etc., can solve the problem of sensitive biological targets yet to be discovered and so on

Pending Publication Date: 2021-12-17
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the development of high-throughput gene sequencing technology, the prediction and precise quantitative detection of lncRNA expression in rat hippocampal tissue can be realized, but sensitive biological targets for learning and memory dysfunction caused by microwave radiation have yet to be discovered

Method used

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  • Target lncRNA: MSTRG.27033.1 for learning and memory dysfunction caused by microwave radiation
  • Target lncRNA: MSTRG.27033.1 for learning and memory dysfunction caused by microwave radiation
  • Target lncRNA: MSTRG.27033.1 for learning and memory dysfunction caused by microwave radiation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1 carries out microwave radiation to rat

[0049] 80 secondary male Wistar rats with a body weight of 220 ± 20 g (Victoria Lihua Company, Beijing) were randomly divided into 4 groups (S, L, C and LC), and the rats in the S group were placed in the radiation box Without radiation, rats in groups L, C and LC were exposed to 10mW / cm 2 Under the conditions of L-band, C-band and L and C-band microwave composite radiation, the radiation time is 6 minutes.

Embodiment 2

[0050] Example 2 rat learning and memory function detection

[0051]The learning and memory functions of the rats in each radiation group after microwave radiation were tested using the Morris water maze test system (Beijing Shuolinyuan Technology, China). The water maze test system is mainly composed of a stainless steel pool, a camera and supporting software. The inner wall of the stainless steel pool is black, with a diameter of 150cm and a height of 50cm. Divide the water maze pool into 4 quadrants, place a 19cm-high black platform in the middle of the first quadrant, pour water into the pool so that the water surface overflows the platform by 1-2cm, and keep the water temperature at 23±2°C. It is stipulated that the center of the pool wall in each quadrant is the water entry point for animals, and the camera is located directly above the pool, and the pool is covered with light-colored curtains to ensure soft and uniform light, the surrounding scenery and furnishings rema...

Embodiment 3

[0058] Embodiment 3 Detection of EEG Activity in Rats

[0059] Select 5 rats in each group, inject 1% pentobarbital sodium 0.5mL / 100g through the intraperitoneal cavity, and the rats are in a state of mild anesthesia (with pain reflex) after 5 minutes, and the MP-150 produced by Biopac Company is used for multiconductor physiological records and As for the analysis system, the electroencephalogram (Electroencephalogram, EEG) of rats was detected 6 hours after exposure. The specific operation process is as follows: use a hair clipper to remove the hair on the top of the head, disinfect and remove fat, place one electrode on the left and right sides of the center of the top of the head, fix it on the scalp with a needle electrode, insert the reference electrode at the edge of the earlobe on the same side, and connect the electrodes EEG amplifier, and divide the EEG graph into Alpha(α), Beta(β), Theta(θ), delta(δ), with a sensitivity of 2000Hz, continuously record the changes of ...

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Abstract

The invention provides a target lncRNA: MSTRG.27033.1 for learning and memory dysfunction caused by microwave radiation. The target comprises at least one of the following sequences: an RNA sequence as shown in SEQ ID NO: 1; or an RNA sequence having at least 70% identity with the RNA sequence as shown in SEQ ID NO: 1, or an RNA sequence having at least 80% identity with the RNA sequence as shown in SEQ ID NO: 1, or an RNA sequence having at least 85% identity with the RNA sequence as shown in SEQ ID NO: 1, or an RNA sequence having at least 90% identity with the RNA sequence as shown in SEQ ID NO: 1, or an RNA sequence having at least 95% identity with the RNA sequence as shown in SEQ ID NO: 1, or an RNA sequence having at least 99% of identity with the RNA sequence as shown in SEQ ID NO: 1.

Description

technical field [0001] The present invention relates to the field of biomedicine. Specifically, the present invention relates to a target lncRNA for learning and memory dysfunction caused by microwave radiation: MSTRG.27033.1. More specifically, the present invention relates to a target lncRNA for learning and memory dysfunction caused by microwave radiation: MSTRG.27033.1, lncRNA: the use of MSTRG.27033.1 as a target for learning and memory dysfunction caused by microwave radiation, a method for detecting learning and memory dysfunction caused by microwave radiation, a method for screening drugs, the use of preparing drugs, and pharmaceutical compositions. Background technique [0002] Microwave technology is widely used in all aspects of production and life, resulting in long-term exposure of workers and the public to the microwave radiation environment. Microwave radiation damage has become a hot issue of social concern. Studies have shown that the brain is one of the mos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12Q1/6883C12Q1/02A61K45/00A61P25/28
CPCC12N15/113C12Q1/6883G01N33/5058A61K45/00A61P25/28C12N2310/14C12Q2600/158C12Q2600/178C12Q2600/136C12N2503/02G01N2500/10C12N2310/531
Inventor 彭瑞云王惠朱睿卿赵黎张静董霁王浩宇徐新萍姚斌伟
Owner ACADEMY OF MILITARY MEDICAL SCI
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