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Recombinant human collagen, coding gene and application of recombinant human collagen to preparation of repair dressing

A recombinant collagen and coding technology, applied in the field of biomedicine, can solve the problems of single molecular weight of hyaluronic acid, the product cannot be sterile, and is not conducive to wound healing, etc., to promote metabolism, improve cell growth microenvironment, and reduce cytotoxins. produced effect

Pending Publication Date: 2021-12-21
山东汉肽医美生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This invention does not use obvious moisturizing ingredients, and cannot provide a long-term moist environment for the wound, which is not conducive to wound healing, and the skin feel is not good
[0008] The recombinant collagen repair dressings disclosed in the above patents all have certain deficiencies. The molecular weight of hyaluronic acid is single, and the moisturizing effect is poor; the product cannot be sterile and antiseptic; the product has a single form, narrow application range, and low application value

Method used

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  • Recombinant human collagen, coding gene and application of recombinant human collagen to preparation of repair dressing
  • Recombinant human collagen, coding gene and application of recombinant human collagen to preparation of repair dressing
  • Recombinant human collagen, coding gene and application of recombinant human collagen to preparation of repair dressing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Weigh 0.45g of large molecular weight HA, 0.15g of medium molecular weight HA, and 0.15g of small molecular weight HA in deionized water at 60°C, stir and mix well, and fully swell.

[0037] (2) Weigh 3.0g of nicotinamide and 2.0g of glycerin into step (1), stir to dissolve.

[0038] (3) Weigh 0.5g of recombinant collagen, add it to step (2), and adjust the volume to 100g to obtain the repair dressing stock solution.

[0039] (4) Add 50 µg of riboflavin to the dressing stock solution to adjust the dressing stock solution to light yellow.

[0040] (5) Filter the raw dressing solution obtained in step (4) with a 10-µm polypropylene filter membrane to remove visible foreign matter, and dispense it into 7 mL vials, each containing 5 mL, seal and press the cap.

[0041] (6) Autoclave the subpackaged dressing solution at 118°C for 16 minutes to obtain a sterile recombinant collagen repair dressing, which provides auxiliary treatment and nursing care for the repair of sma...

Embodiment 2

[0044] (1) Weigh 0.167g of large molecular weight HA, 0.167g of medium molecular weight HA, and 0.167g of small molecular weight HA in deionized water at 50°C, stir and mix well, and fully swell.

[0045] (2) Weigh 1.0g of nicotinamide and 3.0g of glycerin into step (1), stir to dissolve.

[0046] (3) Weigh 0.15 g of recombinant collagen, add it to step (2), and set the volume to 100 g to obtain the repair dressing stock solution.

[0047] (4) Add 75 µg of riboflavin to the dressing stock solution to adjust the dressing stock solution to light yellow.

[0048] (5) Filter the raw dressing solution obtained in step (4) with a 10-µm polypropylene filter membrane to remove visible foreign matter, and dispense it into 30 mL vials with 25 mL per bottle, seal and press the cap.

[0049] (6) Autoclave the subpackaged dressing solution at 121°C for 8 minutes to obtain a sterile recombinant collagen repair dressing, equipped with a spray head, and use it for facial skin problems.

Embodiment 3

[0051] (1) Weigh 0.3g of large molecular weight HA, 0.1g of medium molecular weight HA, and 0.1g of small molecular weight HA in deionized water at 40°C, stir and mix well, and fully swell.

[0052] (2) Weigh 2.0g of nicotinamide and 5.0g of glycerin into step (1), stir to dissolve.

[0053] (3) Weigh 0.3g of recombinant collagen, add it to step (2), and adjust the volume to 100g to obtain the repair dressing stock solution.

[0054] (4) Add 100 µg of riboflavin to the dressing stock solution to adjust the dressing stock solution to light yellow.

[0055] (5) Filter the dressing stock solution obtained in step (4) with a 10µm polyethersulfone filter membrane to remove visible foreign matter, and divide into 30mL vials filled with non-woven fabric, each bottle into 20mL, seal and press the cap .

[0056] (6) Autoclave the subpackaged dressing solution at 118°C for 16 minutes to obtain a sterile recombinant collagen repair dressing, which can be used for facial skin problems. ...

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Abstract

The invention discloses recombinant human collagen, a recombinant collagen repair dressing and a preparation method thereof. The amino acid sequence of the recombinant collagen comprises an amino acid sequence shown as SEQ ID NO: 1, and the protein is high in purity and good in thermal stability. The recombinant collagen repair dressing is obtained by proportioning the recombinant collagen, hyaluronic acid with different molecular weights, other humectants and anti-inflammatory components and then performing moist heat sterilization treatment through a special process. The recombinant collagen repair dressing is sterile and free of preservatives, provides a continuous moist environment for a wound through external prevention and internal supplement to promote wound healing, protect wound surface new tissues and reconstruct a skin barrier function, can be used for adjuvant therapy of non-chronic wound surfaces and small wounds, can also be used for repairing skin injuries caused by skin microenvironment imbalance, is simple in production process and has great application and popularization values.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a recombinant human collagen, an encoding gene and its application in the preparation of repairing dressings. Background technique [0002] Collagen is the most abundant structural protein in animals. It has strong biological activity and biological function. It provides attachment and support for cell growth, induces epithelial cells, fibroblasts, etc. to adhere, proliferate and migrate, and makes connective tissue have mechanical strength. ; At the same time, it has hemostasis, biocompatibility and biodegradability; it can also improve the microenvironment of cell growth, promote the metabolism of skin tissue, repair the skin barrier, and guide the regeneration of body tissue. Based on these characteristics, collagen is widely used in medical and health fields such as burns, trauma, corneal diseases, health care, beauty, orthopedics, hard tissue repair, wound hemostasis, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C12N15/12A61L26/00
CPCC07K14/78A61L26/0033A61L26/0023A61L26/0066A61L26/0061A61L2300/236A61L2300/412A61L2300/252C08L5/08
Inventor 殷世清赵学彩张凤龙蔡永刚魏静孙晖李倩张甲庆李鑫
Owner 山东汉肽医美生物科技有限公司
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