High-resolution quantitative phase microscopic system based on annular LED illumination
A technology of LED illumination and phase microscopy, applied in microscopes, measurement devices, analytical materials, etc., can solve problems such as insufficient detection of the microstructure of organelles, achieve good flexibility and portability, improve immunity, and reduce costs and complexity. degree of effect
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Embodiment 1
[0048] See figure 1 , figure 1 It is a structural schematic diagram of a high-resolution quantitative phase microscopy system based on annular LED illumination provided by an embodiment of the present invention. The high-resolution quantitative phase microscopy system includes a ring illumination module and a petri dish 3 , a detection objective lens 4 , a confocal unit, a spatial light modulator 10 and an image acquisition module 12 sequentially arranged along the optical path direction of the ring illumination module.
[0049] The ring-shaped lighting module includes a light source mounting frame 2 and a plurality of LEDs (light-emitting diodes) 1 evenly arranged on the light source mounting frame 2. The culture dish 3 is arranged directly below the ring-shaped lighting module and is located on the focal plane of the detection objective lens 4. A plurality of The light from the LED 1 can be irradiated on the sample in the petri dish 3 along an oblique direction, thereby pro...
Embodiment 2
[0092] On the basis of the above embodiments, this embodiment provides another high-resolution quantitative phase microscopy system based on annular LED illumination. The high-resolution quantitative phase microscopy system includes a quantitative phase microscopy subsystem and an auxiliary fluorescence subsystem.
[0093] The quantitative phase microscopy subsystem includes a ring illumination module, a petri dish 3 , a detection objective lens 4 , a confocal unit, a spatial light modulator 10 and an image acquisition module 12 sequentially arranged along the optical path direction of the ring illumination module.
[0094] The ring-shaped lighting module includes a light source mounting frame 2 and a plurality of LEDs 1 evenly arranged on the light source mounting frame 2. The culture dish 3 is arranged directly below the ring-shaped lighting module and is positioned on the focal plane of the detection objective lens 4. The light of a plurality of LEDs can be Irradiate on the...
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