High-resolution quantitative phase microscopic system based on annular LED illumination

A technology of LED illumination and phase microscopy, applied in microscopes, measurement devices, analytical materials, etc., can solve problems such as insufficient detection of the microstructure of organelles, achieve good flexibility and portability, improve immunity, and reduce costs and complexity. degree of effect

Active Publication Date: 2021-12-21
XIDIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the spatial resolution and temporal resolution of this system are not enough to detect the fine structure of organelles in living cells, only the overall morphological changes of cells can be detected

Method used

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  • High-resolution quantitative phase microscopic system based on annular LED illumination
  • High-resolution quantitative phase microscopic system based on annular LED illumination
  • High-resolution quantitative phase microscopic system based on annular LED illumination

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Embodiment 1

[0048] See figure 1 , figure 1 It is a structural schematic diagram of a high-resolution quantitative phase microscopy system based on annular LED illumination provided by an embodiment of the present invention. The high-resolution quantitative phase microscopy system includes a ring illumination module and a petri dish 3 , a detection objective lens 4 , a confocal unit, a spatial light modulator 10 and an image acquisition module 12 sequentially arranged along the optical path direction of the ring illumination module.

[0049] The ring-shaped lighting module includes a light source mounting frame 2 and a plurality of LEDs (light-emitting diodes) 1 evenly arranged on the light source mounting frame 2. The culture dish 3 is arranged directly below the ring-shaped lighting module and is located on the focal plane of the detection objective lens 4. A plurality of The light from the LED 1 can be irradiated on the sample in the petri dish 3 along an oblique direction, thereby pro...

Embodiment 2

[0092] On the basis of the above embodiments, this embodiment provides another high-resolution quantitative phase microscopy system based on annular LED illumination. The high-resolution quantitative phase microscopy system includes a quantitative phase microscopy subsystem and an auxiliary fluorescence subsystem.

[0093] The quantitative phase microscopy subsystem includes a ring illumination module, a petri dish 3 , a detection objective lens 4 , a confocal unit, a spatial light modulator 10 and an image acquisition module 12 sequentially arranged along the optical path direction of the ring illumination module.

[0094] The ring-shaped lighting module includes a light source mounting frame 2 and a plurality of LEDs 1 evenly arranged on the light source mounting frame 2. The culture dish 3 is arranged directly below the ring-shaped lighting module and is positioned on the focal plane of the detection objective lens 4. The light of a plurality of LEDs can be Irradiate on the...

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Abstract

The invention discloses a high-resolution quantitative phase microscopic system based on annular LED illumination, and the system comprises an annular illumination module, and a culture dish, a detection objective lens, a confocal unit, a spatial light modulator and an image collection module which are sequentially arranged in the light path direction of the annular illumination module, the annular illumination module comprises a light source mounting rack and a plurality of uniformly arranged LEDs, the culture dish is arranged right below the annular illumination module and located on a focal plane of the detection objective lens, light of the plurality of LEDs can irradiate a sample in the culture dish in an inclined direction to generate non-scattered light and scattered light, and the detection objective lens is used for collecting the scattered light and the non-scattered light; the spatial light modulator is used for performing phase modulation on non-scattered light of the sample, and the image acquisition module acquires a phase shift image. The common-path interference optical structure of the system improves the immunity of the system to external disturbance; and the image quality and the spatial resolution are greatly improved based on the coherent illumination of the multi-angle annular super-oblique part of the LED.

Description

technical field [0001] The invention belongs to the technical field of optical microscopic imaging, and in particular relates to a high-resolution quantitative phase microscopic system based on annular LED illumination. Background technique [0002] Optical microscopy plays an important role in the field of life science research as a non-invasive imaging technology. The principle and structure of the optical microscope are relatively simple, and the requirements for sample preparation are relatively low. The spatial resolution and time resolution can meet the requirements of many applications. It can perform long-term non-invasive detection of multi-scale living samples. It has become an important means for people to understand and study the microcosm. Among them, fluorescence microscopy realizes high-contrast imaging of specific structures through the fluorescence signals generated by the excitation of fluorescent substances. In recent years, through the unremitting effor...

Claims

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Application Information

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IPC IPC(8): G02B21/00G01N21/84
CPCG02B21/0032G02B21/008G01N21/84
Inventor 马英郜鹏郑娟娟马琳刘旻
Owner XIDIAN UNIV
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