Cell absorption combined functional nutrient
A combined and functional technology, applied in the field of food, can solve problems such as adverse stimulation, and achieve the effects of inhibiting secretion, improving nerve growth and development, and improving proliferation and differentiation.
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Embodiment 1
[0029] Ratio culture medium (culture solution): 90% functional nutrients (including 83 parts of nutrient energy supply components, 10 parts of function regulation components, 5 parts of auxiliary material components, and 2 parts of salt) + 10% FBS, tested after mixing The concentrations of various nutrients are shown in Table 1 at time 0 data. Add the prepared medium to the culture dish, insert HaCaT cells, and store at 37°C, pH 7.2-7.4, 5% CO 2 The incubator is sterile and constant temperature culture. 0.5hr, 1hr, 1.5hr, 2hr, 2.5hr, 3hr, 3.5hr, 4hr, respectively, take out part of the culture dish, transfer the cells into the centrifuge tube in the ultra-clean bench for centrifugation, 800 rpm, centrifuge for 5 min, and save the supernatant The test results are shown in Table 1.
[0030] Table 1 Example 1 detects the absorption rate of nutrients by cells in different time periods
[0031]
[0032] The protein in the table is wheat oligopeptide, the fat is glyceryl butyra...
Embodiment 2
[0034] Ratio culture medium (culture solution): 90% functional nutrients (including 80 parts of nutrient energy supply components, 10 parts of function regulation components, 8 parts of auxiliary material components, 2 parts of salt) + 10% FBS, tested after mixing After mixing the concentration of various nutrients, check the concentration of various nutrients and see the data at 0 o'clock in Table 2; add the prepared culture medium to the culture dish, insert HaCaT cells, at 37 ° C, pH value 7.2-7.4, 5 %CO 2 The incubator is sterile and constant temperature culture. 0.5hr, 1hr, 1.5hr, 2hr, 2.5hr, 3hr, 3.5hr, 4hr, respectively, take out part of the culture dish, transfer the cells into the centrifuge tube in the ultra-clean bench for centrifugation, 800 rpm, centrifuge for 5 min, and save the supernatant The test results are shown in Table 2.
[0035] Table 2 Example 2 detects the absorption rate of nutrients by cells in different time periods
[0036]
[0037] The prote...
Embodiment 3
[0039] Ratio culture medium (culture solution): 90% functional nutrients (including 75 parts of nutrient energy supply components, 13 parts of function regulation components, 10 parts of auxiliary material components, 2 parts of salt) + 10% FBS, tested after mixing After mixing the concentration of various nutrients, check the concentration of various nutrients, see the 0 data in Table 3; add the prepared medium to the culture dish, insert HaCaT cells, at 37 ° C, pH value 7.2-7.4, 5% CO 2 The incubator is sterile and constant temperature culture. 0.5hr, 1hr, 1.5hr, 2hr, 2.5hr, 3hr, 3.5hr, 4hr, respectively, take out part of the culture dish, transfer the cells into the centrifuge tube in the ultra-clean bench for centrifugation, 800 rpm, centrifuge for 5 min, and save the supernatant The test results are shown in Table 3.
[0040] Table 3 Example 3 detects the absorption rate of nutrients by cells in different time periods
[0041]
[0042] The protein in the table is whea...
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