Culture device for preparing mesenchymal stem cell exosomes

Abstract

The invention discloses a culture device for preparing mesenchymal stem cell exosomes. The culture device comprises a fixed shell assembly and is characterized in that the fixed shell assembly comprises a fixed box; the front surface of the fixed box is movably sleeved with a push-pull door plate; the front surface of the push-pull door plate is fixedly connected with a U-shaped handle; a first fixing plate is fixedly connected to the position, close to the back face, of the bottom of the inner side of the fixed box; a movable nutrient solution injection assembly is fixedly connected to the top of the inner side of the fixed box and comprises a second fixing plate, and the two sides of the front face of the second fixing plate are each fixedly connected with two first sliding rods; a first sliding block is movably connected to the outer side of each first sliding rod in a sleeving mode; and a third fixing plate is fixedly connected to the front face of the fixed box, so that a nutrient solution can be added into cells in a stem cell culture dish under the condition that a sterile room is not opened; and the condition that cells are polluted by bacteria and molds in the air when a worker adds the nutrient solution is reduced.

Description

technical field [0001] The invention relates to the technical field of cell culture, and more specifically to a culture device for preparing exosomes of mesenchymal stem cells. Background technique [0002] Stem cells are a kind of pluripotent cells with self-replication ability. Under certain conditions, they can differentiate into a variety of functional cells. According to the developmental stage of stem cells, they can be divided into embryonic stem cells and adult stem cells. According to the developmental potential of stem cells, they can be divided into three types: Classes: totipotent stem cells, pluripotent stem cells and unipotent stem cells. Stem cells are not fully differentiated and immature cells that have the potential to regenerate various tissues, organs and the human body. They are called "universal cells" in the medical field. [0003] Stem cell culture means that after the tissue is taken out from the donor, it is mechanically and digested to separate int...

AI Technical Summary

Problems solved by technology

[0008] One: Bacterial or mold contamination is a common cause of culture failure. It is necessary to strengthen the concept of aseptic operation in each link, focusing on prevention. Once contamination is generally difficult to eliminate, but in the process of existing cell culture, staff need to manually Add nutrient solution to the petri dish. Manual addition will expose the petri dish to the outside air, which may be infected by bacteria and mold in the air.

[0009] Second: Stem cells should be placed in the CO2 incubator for the first 24 to 48 hours (72 hours if necessary) after the stem cells are prevented from being cultured. It is difficult to adhere to the wall, let alone stretch and proliferate. There is no need to worry that the nutrients in the culture medium will be exhausted. The requirement for nutrients before cell proliferation is not large. The purpose of adding a thin layer of culture medium at the initial stage of primary culture is also Because it is conducive to cell adhesion and extension, the CO2 concentration in the CO2 incubator must be kept between 5% and 8%. When the CO2 incubator is filled with air, bacteria and mold in the air will be brought into the CO2 incubator, affecting stem cells. cultivation

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