Construction method of wasp wine fingerprint spectrum and application method of wasp wine fingerprint spectrum in quality detection of wasp wine
A technology of fingerprint spectrum and construction method, which is applied in the field of quality inspection of vespa wine and the construction of vespa wine fingerprint spectrum, and achieves the effects of good reproducibility, high popularization and application value, and high stability
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Embodiment 1
[0039] Example 1 Construction of the fingerprint of Vespa wine
[0040] A, preparation of Vespa wine: collect fresh Vespa ( Vespa mandarinia Smith), the specific sample information is shown in Table 1, weighed 100g, added 1000mL white wine, soaked for more than 20 days, and obtained the wasp wine for later use.
[0041] B. Preparation of the test solution: Measure 2 mL of Vespa wine, concentrate and dry under reduced pressure at 60°C to obtain a residue, add 10 mL of 0.1 mol / L hydrochloric acid solution to dissolve the residue, shake well and set aside.
[0042] Measure 5 mL of each of the above solutions, add 2.5 mL of 0.1 mol / L phenyl isothiocyanate in acetonitrile, 2.5 mL of 1 mol / L triethylamine in acetonitrile, shake well, let stand at room temperature for 1 hour, then add 50% acetonitrile Dilute to 25mL, shake again and set aside.
[0043] Measure 10 mL of each of the above solutions, add 10 mL of n-hexane each, shake well, let stand for 10 minutes, remove the lower l...
Embodiment 2
[0052] The precision test of the construction method described in embodiment 2
[0053] Get the vespaste wine (sample serial number S1) prepared in Example 1, prepare the test solution according to the procedure described in Example 1, inject samples continuously for 6 times according to the chromatographic conditions selected in Example 1, detect the fingerprints, and use the No. 16 chromatographic peak For reference, the relative retention times and relative peak areas were obtained by comparing the other respective peaks. The relative retention time RSD of each chromatographic peak was ≤0.38%, and the relative retention peak area RSD was ≤0.41%, indicating that the precision of the fingerprint construction method was good.
Embodiment 3
[0054] The stability test of the construction method described in embodiment 3
[0055] Take the Vespa wine (sample number S1) prepared in Example 1, prepare the test solution according to the procedure described in Example 1, and inject samples at 0, 2, 4, 8, 24, and 48 hours according to the chromatographic conditions selected in Example 1 , with No. 12 chromatographic peak as a reference, other peaks are compared with it to obtain relative retention time and relative peak area. The relative retention time RSD of each chromatographic peak≤0.45%, and the relative retention peak area RSD≤0.81%, show that the need testing solution prepared according to the procedure remains stable within 48h, and the construction method of the fingerprint is stable.
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