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Intermediate infrared rapid batch detection method for alpha s1-casein in milk

A batch detection and casein technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of long time-consuming determination, high cost, complicated operation, etc., and achieve the effect of fast identification, low cost, and simple operation

Active Publication Date: 2022-01-11
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the problems of long time-consuming, high cost and complicated operation in casein detection, and provide a casein in milk with fast identification speed, high precision, low cost, simple operation, batch detection and strong practicability Mid-infrared detection method of content

Method used

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  • Intermediate infrared rapid batch detection method for alpha s1-casein in milk
  • Intermediate infrared rapid batch detection method for alpha s1-casein in milk

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Establishment of a method for detecting αs1-casein content in milk by infrared spectroscopy:

[0029] Milk samples from 6 different dairy farms in 4 different regions were selected and numbered. Pour the sample into a cylindrical sample tube with a diameter of 3.5cm and a height of 9cm, bathe in a water bath at 42°C for 15-20min, and use MilkoScan from FOSS Company TM 7RM milk component detector, the solid fiber optic probe is extended into the liquid, the sample is scanned, and the light transmittance of the milk sample is obtained through the computer ( figure 1 ). Due to 4089.48cm -1 -2758.47cm -1 , 3005-3680cm -1 The spectral information fluctuates greatly in the wavenumber range, and contains a large amount of noise data. If it is used for subsequent analysis and modeling, the generalization ability of the model will be deteriorated; 2758.47cm -1 -1716.81cm -1 , 3680cm -1 -4000cm -1 There is less effective information in the wavenumber range, and the contri...

Embodiment 2

[0046] alpha in milk s1 -Application of the mid-infrared rapid batch detection method for casein:

[0047] Take 20 α s1 - Casein samples to make predictions for the model. Comparison of predicted results with real values ​​(reverse-phase high-performance liquid chromatography, RP-HPLC) (Wang Hao, Zhang Zhiguo, Chang Yanzhong, Duan Xianglin, Zhao Shuqiang, Zhang Nan, Shi Zhenhua. Separation of main milk proteins in dairy products by RP-HPLC And quantitative determination [J]. Food Science, 2009, 30 (24): 376-380.) comparison.

[0048] Method: Collect the spectrum of the sample according to the method in Example 1, and substitute the spectral data into the SG1-CARS-SVR model constructed in Example 1 to output the prediction results, as shown in the table. Rp=0.8998, RMSEP=1.3726.

[0049]

[0050]

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Abstract

The invention belongs to the field of dairy cow performance determination and milk quality detection, and discloses an intermediate infrared rapid batch detection method for alpha s1-casein in milk. According to the intermediate infrared rapid batch detection method, on the basis of data obtained by an infrared spectrometer in original Fourier transform, wave bands with much noise and less effective information are removed, manual dimension reduction is carried out, and the intermediate infrared rapid batch detection method for the alpha s1-casein in the milk is finally obtained by preprocessing spectral data through using an SG convolution first-order derivative, extracting intermediate infrared spectral characteristic variables through a competitive adaptive reweighting method, and constructing a regression model by using training set data through a support vector machine regression algorithm. The intermediate infrared rapid batch detection method has the advantages of high identification speed, high precision, low cost, simplicity in operation, batch detection, high practicability and the like, and is suitable for large-scale popularization.

Description

technical field [0001] The invention belongs to the field of dairy cow performance measurement and milk quality detection, and in particular relates to a mid-infrared rapid batch detection method for αs1-casein in milk. Background technique [0002] Milk is rich in milk protein, enzymatically digested milk protein is a major source of bioactive peptides that positively affect body functions, support the growth of bifidobacterial species, inhibit the adhesion of bacteria and viruses in the jejunal mucus and act as an anti-inflammatory and immune modulators (Jiehui Z, Liuliu M, Haihong X, et al. Immunomodulating effects of casein-derived peptides QEPVL and QEPV on lymphocytes in vitro and in vivo[J]. Food&function, 2014, 5(9): 2061-2069. Ferrario, C., Duranti, S., Milani, C., Mancabelli, L., Lugli, G.A., Turroni, F., Mangifesta, M., Viappiani, A., Ossiprandi, M.C., van Sinderen, D., and Ventura, M.Exploring amino acid auxotrophy in Bifidobacterium bifidum PRL2010.Front.Microb...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/3577
CPCG01N21/3577G01N2201/129G01N2021/3595
Inventor 张淑君肖仕杰王巧华陈冬梅李春芳倪俊卿马亚宾阮健熊琪陈明新刘锐郝兴杰胡修忠万平民苏俊东
Owner HUAZHONG AGRI UNIV
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