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Application of ginseng-radix aconiti lateralis praeparata heart-strengthening pill in promotion of myocardial cell autophagy

A technique for cardiomyocytes and strong heart pills, which is used in medical preparations containing active ingredients, pill delivery, pharmaceutical formulations, etc.

Inactive Publication Date: 2022-01-18
津药达仁堂集团股份有限公司达仁堂制药厂
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Problems solved by technology

In the prior art, there is no research on the effect of Shenfu Qiangxin Pill on the autophagy of cardiomyocytes of H9C2 rats treated with OGD, and whether it affects the crosstalk of autophagy and apoptosis

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  • Application of ginseng-radix aconiti lateralis praeparata heart-strengthening pill in promotion of myocardial cell autophagy
  • Application of ginseng-radix aconiti lateralis praeparata heart-strengthening pill in promotion of myocardial cell autophagy
  • Application of ginseng-radix aconiti lateralis praeparata heart-strengthening pill in promotion of myocardial cell autophagy

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Embodiment Construction

[0021] Unless otherwise defined, the technical terms used in the following embodiments have the same meaning as commonly understood by those skilled in the art to which the present invention belongs. The test reagents used in the following examples, unless otherwise specified, are conventional biochemical reagents; the experimental methods, unless otherwise specified, are conventional methods.

[0022] The present invention will be described in detail below in conjunction with the embodiments and accompanying drawings.

[0023] The present invention intends to start with the interactive genes that affect autophagy and apoptosis crosstalk, and perform oxygen-glucose deprivation (OGD) treatment on H9C2 rat cardiomyocytes, through CCK-8 and lactate dehydrogenase (lactatedehydrogenase, LDH) To screen the optimal intervention time of OGD and the optimal concentration of Shenfu Qiangxin Pills, and to treat with autophagy inducers and autophagy inhibitors to study the effect of Shenf...

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Abstract

The invention provides application of a ginseng-radix aconiti lateralis praeparata heart-strengthening pill in promotion of myocardial cell autophagy. An H9C2 rat myocardial cell damage model is established through OGD; WB detection shows that the ginseng-radix aconiti lateralis praeparata heart-strengthening pill can promote autophagy of H9C2 myocardial cells; through addition of an autophagy inhibitor and an autophagy inducer, it is determined that one of protection mechanisms of the ginseng-radix aconiti lateralis praeparata heart-strengthening pill in inhibiting OGD-induced cell apoptosis may up-regulate the activity of autophagy, which shows interaction between autophagy and apoptosis; and it is also shown that the ginseng-radix aconiti lateralis praeparata heart-strengthening pill may be related to an MST1 / JNK signal pathway in regulating and controlling interaction between autophagy and apoptosis of H9C2 rat myocardial cells.

Description

technical field [0001] The invention belongs to the technical field of cell autophagy, and in particular relates to the application of Shenfu Qiangxin Wan in promoting cardiomyocyte autophagy. Background technique [0002] Network pharmacology combined with metabolomics studies have shown that MAPK1 / 3 (ERK1 / 2) is the core target of Shenfu Qiangxin Pills in the treatment of heart failure. Previous studies have shown that Shenfu Qiangxin Pills can regulate MAPK signaling pathways (reduce JNK, ERK and p38 expression) inhibit cardiorenal cell apoptosis and play a role in the treatment of cardiorenal syndrome. The MST1 / JNK signaling pathway plays an important role in heart failure. MST1 is the upstream of the MAPK signaling pathway, and JNK is the switch of the MAPK signaling pathway. Both MST1 and JNK can play an important role in autophagy and apoptosis. Beclin 1 is a dual regulator of autophagy and apoptosis, and is closely related to Caspase-3, Bcl-2, and Bax. In the prior ...

Claims

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Application Information

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IPC IPC(8): A61K36/714A61K9/20A61P9/04
CPCA61K36/258A61K36/714A61K36/605A61K36/07A61K36/708A61K9/20A61P9/04A61K2300/00
Inventor 吴芸蛟邵凤袁婷婷曲磊王婉婷赵微孟亚飞
Owner 津药达仁堂集团股份有限公司达仁堂制药厂
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