Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of tumor neoantigen specific T cells

A specific and cell-based technology, applied in the field of cell biology, can solve the problems of low proportion of tumor neoantigen-specific T cells, limited killing effect of tumor cells, and low purity of mature DC cells, so as to achieve strong specific immune response and good stimulation Immune system, effect of improving phagocytosis efficiency

Pending Publication Date: 2022-01-18
HANGZHOU NEOANTIGEN THERAPEUTICS CO LTD
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The immature DC cells obtained by this method have low purity due to containing T cells; directly lead to the low purity and small number of mature DC cells obtained by inducing immature DC cells; finally, tumor neogenesis obtained in the co-cultured cell suspension The proportion of antigen-specific T cells is extremely low, and the killing effect on tumor cells is very limited

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of tumor neoantigen specific T cells
  • Preparation method of tumor neoantigen specific T cells
  • Preparation method of tumor neoantigen specific T cells

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0053] A method for preparing tumor neoantigen-specific T cells and verifying their effectiveness in killing tumor cells, comprising:

[0054] Step 1, synthesizing tumor neoantigen polypeptide;

[0055] For specific methods, please refer to a screening method for individualized tumor neoantigen peptides in patent 202110251383.X to obtain polypeptide sequences, and then synthesize polypeptides, and use solid-phase synthesis technology to produce suitable purity polypeptides; to verify the immune function of the obtained polypeptides:

[0056] Blood samples from healthy volunteers were drawn to separate PBMCs, and the immune function of the peptide vaccine was verified in vitro, and the secretion of IFN-γ after peptide stimulation was detected. Using the ELISpot method, with the polypeptide vaccine as the stimulus, the long polypeptide of the present invention as the experimental group 1, the corresponding short polypeptide at the same site in the long polypeptide as the experim...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of tumor neoantigen specific T cells, which comprises the following steps: step 1, obtaining biological information through gene sequencing, and designing and synthesizing tumor neoantigen polypeptide; step 2, loading the tumor neoantigen polypeptide to immature DC cells, and inducing the DC cells to mature by using cytokines; step 3, performing multi-round co-incubation stimulation on the mature DC cells and the initial T cells; step 4, separating and collecting tumor neoantigen specific T cells by using an immunomagnetic bead technology; step 5, amplifying to obtain tumor neoantigen specific T cells with high purity and killing activity; step 6, performing cryopreservation to obtain tumor neoantigen specific T cells; according to the method disclosed by the invention, a large number of tumor neoantigen specific T cells with high purity and high killing activity can be efficiently prepared, and the tumor killing efficiency is close to 100%; the cost is low, the speed is high, and market requirements are met.

Description

technical field [0001] The invention relates to the technical field of cell biology, in particular to a method for preparing tumor neoantigen-specific T cells. Background technique [0002] Recently, due to breakthroughs in the treatment of malignant melanoma and blood system tumors, adoptive immune cell therapy has once again become a hot spot in the field of tumor immunotherapy research. At present, the commonly used effector cells of adoptive immune cell therapy in the world mainly include tumor infiltrating lymphocytes (Tumor Infiltrating Lymphocyte, abbreviated as TIL), cytokine-induced killer cells (Dendritic Cell-Cytokine Induced Killer, abbreviated as DC-CIK), chimeric Antigen receptor modified T cells (Chimeric Antigen Receptor T-Cell, abbreviated as CAR-T) and T cell receptor genetically engineered T cells (T-Cell Receptor gene engineered T-cell, abbreviated as TCR-T). For adoptive immune cell therapy, the acquisition of highly specific tumor antigen targets is ex...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0783C12N5/0784
CPCC12N5/0636C12N2502/1121C12N2501/599C12N2501/25C12N2501/2301C12N2501/2306C12N2501/02C12N2501/999C12N2501/515C12N2501/51C12N2501/52C12N2501/2302C12N2501/2307C12N2501/2315
Inventor 莫凡刘亮丁冬
Owner HANGZHOU NEOANTIGEN THERAPEUTICS CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com