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Antibacterial peptide RLR derived from sheep source as well as preparation method and application thereof

An antibacterial peptide, Gram-negative bacteria technology, applied in the direction of cationic antibacterial peptides, antibacterial drugs, chemical instruments and methods, etc. Action, the effect of narrowing the antibacterial spectrum

Active Publication Date: 2022-01-21
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The goat-derived antimicrobial peptide ChBac5 has a length of 43 amino acids, a complex structure, and a broad antibacterial spectrum, which is not conducive to production and application

Method used

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  • Antibacterial peptide RLR derived from sheep source as well as preparation method and application thereof
  • Antibacterial peptide RLR derived from sheep source as well as preparation method and application thereof
  • Antibacterial peptide RLR derived from sheep source as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Design of Antimicrobial Peptides

[0015] The amino acid sequence of the goat-derived antimicrobial peptide ChBac5 is:

[0016] RFRPPIRRPPIRPPFNPPFRPPVRPPFRPPFRPPFRPPIGPFP

[0017] By intercepting 16 amino acids at the end of the goat-derived antimicrobial peptide ChBac5, a polypeptide RFR containing 5 positive charges and a hydrophobic value of 3.66 was obtained, and its amino acid sequence was: RFRPPIRRPPPIRPPFN;

[0018] Replace the double Pro-Pro of the RFR rigid structure with Ala that promotes α-helix, and replace the terminal Asn with Arg to further increase the positive charge content of the antimicrobial peptide, and obtain a polypeptide containing 6 positive charges and a hydrophobic value of 0.186 RFRR, its amino acid sequence is: RFRAAIRRAAIRAAFR;

[0019] Ile and Lue were used to replace the double Ala-Ala on both sides to increase hydrophobicity, and Lue was used to replace Phe to reduce cytotoxicity, and finally the C-terminal was amidated to increase o...

Embodiment 2

[0023] Determination of antimicrobial activity of antimicrobial peptides

[0024] 1. Determination of antibacterial activity: the minimum inhibitory concentration of the antimicrobial peptide RLR was determined by the micro broth dilution method. Using 2 mg / ml BSA (containing 0.01% acetic acid) as the diluent, a series of gradient antimicrobial peptide solutions were sequentially prepared using the double dilution method. Take 100 μL of the above solution and place it in a 96-well cell culture plate, then add an equal volume of the bacteria solution to be tested (~10 5 individual / mL) in each well. Positive controls (containing bacterial fluid but not antimicrobial peptides) and negative controls (neither bacterial fluid nor antimicrobial peptides) were set up. Incubate at a constant temperature of 37°C for 14-18h, and use a microplate reader at 492nm (OD 492nm ) to determine the minimum inhibitory concentration. The test results are shown in Table 2.

[0025] Antibacteria...

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Abstract

The invention provides an antibacterial peptide RLR derived from a sheep source as well as a preparation method and application thereof. The sequence of the antibacterial peptide RLR is shown as SEQ ID No.1 in a sequence table. The preparation method comprises the following steps: intercepting 16 amino acids at the tail end of a sheep-derived antibacterial peptide ChBac5 to obtain a polypeptide RFR; replacing double Pro-Pro of an RFR rigid structure with Ala for promoting alpha-helix, and replacing Asn at the tail end with Arg to obtain a polypeptide RFRR; using Ile and Lue to replace double Ala-Ala on two sides, Phe is replaced by Lue, and finally, performing C-terminal amidation to obtain the antibacterial peptide RLR. The antibacterial peptide RLR is applied to preparation of medicines for treating gram-negative bacteria infectious diseases. The antibacterial peptide has an efficient inhibition effect and extremely low hemolytic activity on gram-negative bacteria, but does not damage gram-positive bacteria, so that the antibacterial peptide has the development potential of becoming a narrow-spectrum antibiotic substitute.

Description

technical field [0001] The invention belongs to the application field of agricultural animal husbandry and veterinary medicine, and in particular relates to an antimicrobial peptide RLR derived from goat source and its preparation method and application. Background technique [0002] Antimicrobial peptides are a kind of polypeptides with antibacterial activity that widely exist in organisms. They also have killing effects on fungi, viruses, parasites and cancer cells. Some antimicrobial peptides also have multiple functions such as chemotaxis, promoting wound healing and regulating immunity. So far, it is generally believed that antimicrobial peptides mainly act on the cell membrane of bacteria, causing the leakage of cell contents through the destruction of the membrane, leading to cell death. The special mechanism of action of antimicrobial peptides greatly reduces the possibility of bacteria developing drug resistance through genetic mutations. Therefore, the study of an...

Claims

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Application Information

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IPC IPC(8): C07K14/47A61K38/17A61P31/04
CPCC07K14/4723A61P31/04A61K38/00Y02A50/30
Inventor 李仲玉李玲董娜方禹鑫朱允慧
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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