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Artificial design and application of intercellular communication system

A communication system and cell technology, applied in the fields of bioengineering and genetic engineering, can solve problems such as difficult transformation and unclear biosynthetic pathways, and achieve the effect of improving predictability

Pending Publication Date: 2022-01-21
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Abstract
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  • Application Information

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Problems solved by technology

However, since the complete biosynthetic pathways of signaling molecules in these systems are either unclear or difficult to transfer to another species [2-3], their modularity and transferability are limited to a few specific cell types or must be performed in culture Replenishes its essential precursors for synthesis of this signaling molecule in host cells

Method used

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  • Artificial design and application of intercellular communication system
  • Artificial design and application of intercellular communication system
  • Artificial design and application of intercellular communication system

Examples

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Embodiment

[0119] Design concepts for signal transmitting and / or signal receiving components

[0120] For DAPG, pC, IV, Sal, MMF, and NG transmitters and receivers ( figure 2 a), design optimization based on the following concepts.

[0121] Sal signaling: putative Pseudomonas putida promoter P reported sal (SEQ ID NO:54) is active in E. coli [28]. The inventors of the present invention have found that Sal that expresses both isochorisate-pyruvate lyase (AEO73196.1) and isochorisate synthase (WP_003114686.1), or only salicylate synthase (WP_005168693.1) Transmitter cells can be activated with P sal Receiver cells for inducible promoters ( figure 2 a).

[0122] DAPG signaling: It has been reported in the art that the use of P phlF (SEQ ID NO: 53) to screen TetR family repressors [29]. We heterologously expressed the phlACBD gene cluster of Pseudomonas fluorescens in E. coli. Co-culture experiments showed that DAPG receivers can be activated by DAPG transmitters.

[0123] NG sign...

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Abstract

The invention relates to a brand new design method of an intercellular communication system, the intercellular communication system designed according to the method and application thereof. Specifically, the intercellular communication system is composed of a signal transmitting element and a signal receiving element, and the signal transmitting element and the signal receiving element are located in different cells; wherein the signal transmitting element is a signal molecule biosynthesis gene expression cassette; the signal receiving element comprises an allosteric transcription factor expression cassette and an inducible promoter; wherein the allosteric transcription factor interacts with the signal molecule, and the inducible promoter comprises a manipulation sequence interacting with the allosteric transcription factor; wherein the signal molecule is selected from one or more of 2, 4-diacetyl phloroglucinol (DAPG), p-coumaroyl-homoserine lactone (pC), isovaleryl-homoserine lactone (IV), salicylate (Sal), methylenefuran (MMF) and naringenin (NG).

Description

technical field [0001] The invention relates to the fields of biological engineering and genetic engineering. More specifically, the present invention provides a new (de novo) design method for intercellular communication systems, an intercellular communication system designed according to the method and verified by experiments, and the system is useful in constructing multicellular signaling networks and engineered multicellular use in the organization. Background technique [0002] Cell-cell communication is critical for multicellular group behaviors such as division of labor, cooperation, and development into tissues. Intercellular signaling systems that mediate such functions of information transmission and signal exchange exist widely in both prokaryotes and eukaryotes. For example, the quorum-sensing system of prokaryotic cells coordinates the behavior of individual cells in the population by means of secreted signaling molecules to form biofilms, achieve bioluminesc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/63C12N5/10G16B5/00
CPCC12N15/63C12N9/0008C12N9/88C12N9/1037C12N9/1029C12N9/90C07K14/4702C12Y402/99021C12Y203/01074C12Y505/01006G16B5/00
Inventor 娄春波杜沛张浩千赵会伟陶勇项延会钱珑
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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