Construction method of infectious anal fistula animal model

Abstract

The invention relates to a construction method of an infectious anal fistula animal model and application of the animal model in a method for screening anal fistula injury treatment drugs or simulating clinical administration and curative effect evaluation. The preparation method comprises the steps that a PVC wrapped aluminum wire penetrates through the anal sphincter from the position about 1 cm away from the left side or the right side of the anus, so that the PVC wrapped aluminum wire is in a virtual hanging state around the anal sphincter; meanwhile, fresh 2-5% dextran sodium sulfate salt drinking water is replaced for an animal to be modeled; after about one week, purulent secretions exist on a wound and a large amount of fecal secretions overflow when the periphery of a fistula is slightly pressed; and meanwhile, through magnetic resonance imaging (MRI) examination and pathological analysis results, it is shown that high-signal-shadow line-shaped fistula formation and pathological examination conform to pathological changes of anal fistula. The method is high in modeling success rate, good in stability, easy to operate, economical and applicable, more importantly, can simulate the clinical anal fistula pathogenesis, is very suitable for clinical research on anal fistula diseases, and solves the problem that there is no reasonable anal fistula animal model at present.

Description

technical field [0001] The invention relates to the research field of medical animal models, in particular to a method for constructing an animal model of infectious anal fistula. Background technique [0002] Anal fistula is a recurrent anorectal disease and belongs to a chronic infectious pipeline. The clinical manifestations are recurrent perianal infection, ulceration, pus, pain, itching and other local symptoms. Multiple fistulas, that is: several external openings or several fistulas, if there is only one, it is called simple anal fistula, and if there are multiple, it is called complex anal fistula. At present, there are many surgical methods for the treatment of anal fistula at home and abroad, such as: incision and drainage, anal fistula incision, fistula removal, thread setting, fistula packing, etc. These methods all have a certain recurrence rate, secondary infection and anal fistula. Incontinence and other issues. So far, there is still a lack of "standard sur...

AI Technical Summary

Problems solved by technology

Previously, some researchers reported several modeling methods: ① animal body surface fistula model, which is not strictly an anal fistula animal model, because the fistula is not related to the anus; ② animal anal canal drainage line fistula model, which is The consistency of the degree of fistula epithelialization in the model is difficult to unify, and it is related to the drainage time and the material of the drainage line. If the experimental time is prolonged, whether the fistula epithelialization will appear remains to be studied; The wound surface is coated with standard Staphylococcus aureus or covered with fresh human feces suspension, which can be used to study wound infection and repair, but has no correlation with the formation of anal fistula; The steel wire (steel needle) passes through the anal sphincter to form a virtual hanging drainage. Some studies have shown that after 30 days of modeling (the molding cycle is longer), there is no sign of infection and corrosion in the perianal general observation, and the threaded steel wire (steel needle) is easy to be damaged. Perianal tissue surrounds and forms a closed state, and the threaded steel wire cannot be removed for follow-up evaluation and treatment

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