Antibacterial peptide hrNCM as well as preparation method and application thereof

A technology for solid-phase synthesis of antimicrobial peptides and polypeptides, applied in the field of biomedicine, can solve the problems of high cytotoxicity, low antibacterial activity, and poor stability, and achieve the effects of low hemolytic activity, small molecular weight, and high stability

Active Publication Date: 2022-02-01
苏州市第九人民医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, some natural antimicrobial peptides still have problems s...

Method used

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  • Antibacterial peptide hrNCM as well as preparation method and application thereof
  • Antibacterial peptide hrNCM as well as preparation method and application thereof
  • Antibacterial peptide hrNCM as well as preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0019] Chemical Synthesis of the Antimicrobial Peptide hrNCM from the Transformed Green Sea Turtle

[0020] The green sea turtle antimicrobial peptide Cm-CATH2 is a polypeptide encoded by a gene, containing 33 amino acid residues, a molecular weight of 4089.9Da, and an isoelectric point of 12.96. The full sequence of the green sea turtle antimicrobial peptide Cm-CATH2 is: Arg 1 Arg 2 Ser 3 Arg 4 Phe 5 Gly 6 Arg 7 Phe 8 Phe 9 Lys 10 Lys 11 Val 12 Arg 13 Lys 14 Gln 15 Leu 16 Gly 17 Arg 18 Val 19 Lys 20 Arg 21 His 22 Ser 23 Arg 24 Ile 25 Thr 26 Val 27 Gly 28 Gly 29 Arg 30 met 31 Arg 32 Phe 33 (SEQ ID NO. 1). According to the amino acid sequence of the green sea turtle antimicrobial peptide Cm-CATH2, a series of peptide chain shortening peptides were designed by molecular modification methods, and the modified N-CM4 was screened out through research on antibacterial, anti-inflammatory and cytotoxic hemolytic activities, ...

Embodiment 2

[0028] hrNCM pharmacological experiments:

[0029] 1. Determination of antibacterial activity of hrNCM:

[0030] (1) Pick the test strains preserved on the slant and spread them evenly on the MH solid culture medium (Beijing Suo Laibao Technology Co., Ltd.) plate, place the sterilized 0.5cm diameter filter paper on the surface of the culture medium, Add 10 μl of 2 mg / ml antibacterial peptide hrNCM sample solution dissolved in sterilized deionized water dropwise, incubate upside down at 37°C for 18-20 hours, and observe whether the antibacterial zone is formed or not. If the sample has antibacterial activity, a clear and transparent bacteriostatic zone will be formed around the filter paper, and the larger the bacteriostatic zone, the stronger the antibacterial activity of the sample.

[0031] (2) Determination of Antimicrobial Peptide hrNCM Minimum Inhibitory Concentration (2-fold dilution method):

[0032] Select the strains with the inhibition zone in the previous experime...

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Abstract

The invention discloses an antibacterial peptide hrNCM as well as a preparation method and an application thereof. According to an amino acid sequence of the green sea turtle antibacterial peptide, a modified body hrNCM is designed by utilizing a molecular modification method, and the modified body has broad-spectrum and efficient antibacterial activity and very strong anti-inflammatory activity and also has the beneficial characteristics of small molecular weight, simple structure, low hemolytic activity, simple preparation method, high stability and the like.

Description

technical field [0001] The invention relates to an antibacterial peptide hrNCM, a preparation method and application thereof, and belongs to the technical field of biomedicine. Background technique [0002] In recent years, the large-scale development and abuse of traditional antibiotics has led to more and more serious problems of drug resistance of pathogenic microorganisms, posing a huge threat to human health. The clinical response to drug-resistant microbial infections is to use new or alternative antibiotics that have not been used against drug-resistant microorganisms, so this requires the continuous development of new antimicrobial drugs. [0003] Antimicrobial peptides are natural small molecular polypeptides encoded by the genes of organisms, and are important molecules in the immune system of organisms. They have direct killing effects on bacteria, fungi, viruses and even protozoa. Antimicrobial peptides have the advantages of small molecular weight, simple struc...

Claims

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Application Information

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IPC IPC(8): C07K14/46C07K1/04C07K1/06C07K1/20A61K38/17A61P31/04A61P31/10A61P29/00A23L3/3535A23K20/147A61K8/64A61Q17/00
CPCC07K14/463A61P31/04A61P31/10A61P29/00A61K8/64A61Q17/005A23L3/3535A23K20/147A61K38/00A23V2002/00A23V2200/10A23V2250/55Y02A50/30
Inventor 李斌高泉根沈根海王义鹏欧阳建红
Owner 苏州市第九人民医院
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