DNA hydrogel, preparation method, hydrogel composition and application of hydrogel and hydrogel composition
A hydrogel and DNA-1 technology, applied in the field of hydrogel composition and DNA hydrogel, can solve the problems of polluted cells, cell influence, and low cell purity, and achieve strong designability, improved efficiency, and biological good compatibility
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Embodiment 1
[0056] In this example:
[0057] Circular DNA template—made by the following method:
[0058] The sequences of ssDNA-1 modified by phosphorylation at the 5' end, primer 1, ssDNA-2 modified by phosphorylation at the 5' end, primer 2 and PD-1 adapter are shown in Table 1:
[0059] Table 1. DNA sequences
[0060]
[0061]
[0062] Preparation of circular DNA template one:
[0063] Design and synthesize ssDNA-1 modified by phosphorylation at the 5' end, the number of bases of ssDNA-1 is 106, design primer 1 of ssDNA-1, the number of bases of primer 1 is 23, the 5' end and 3' of ssDNA-1 The ends are complementary to the 3' end and 5' end of primer 1, respectively.
[0064] The nucleotide sequence of ssDNA-1 is shown in SEQ ID NO.1, see Table 1.
[0065] The nucleotide sequence of primer 1 is shown in SEQ ID NO.3, see Table 1.
[0066] The complementary sequence of the PD-1 aptamer is shown in SEQ ID NO.5, see Table 1.
[0067] Mix ssDNA-1 modified by phosphorylation at ...
Embodiment 2
[0075] In this example:
[0076] Circular DNA template—made by the following method:
[0077] The sequences of ssDNA-1 modified by phosphorylation at the 5' end, primer 1, ssDNA-2 modified by phosphorylation at the 5' end, and primer 2 are shown in Table 2:
[0078] Table 2. DNA sequences
[0079]
[0080] Preparation of circular DNA template one:
[0081] Design and synthesize ssDNA-1 modified by phosphorylation at the 5' end, the base number of ssDNA-1 is 92, design primer 1 of ssDNA-1, the base number of primer 1 is 16, the 5' end and 3' end of ssDNA-1 The ends are complementary to the 3' end and 5' end of primer 1, respectively.
[0082] The nucleotide sequence of ssDNA-1 is shown in SEQ ID NO.6, see Table 1.
[0083] The nucleotide sequence of primer 1 is shown in SEQ ID NO.8, see Table 1.
[0084]Mix ssDNA-1 modified by phosphorylation at the 5' end and primer 1 at a molar ratio of 1:1.5, add NaCl at a final concentration of 70mmol / L, use sterile water to make th...
Embodiment 3
[0092] In this example:
[0093] Circular DNA template-made by the following method:
[0094] The sequences of ssDNA-1 modified by phosphorylation at the 5' end, primer 1, ssDNA-2 modified by phosphorylation at the 5' end, and primer 2 are shown in Table 3:
[0095] Table 3. DNA sequences
[0096]
[0097]
[0098] Preparation of circular DNA template one:
[0099] Design and synthesize ssDNA-1 phosphorylated at the 5' end, the base number of ssDNA-1 is 158, design primer 1 of ssDNA-1, the base number of primer 1 is 37, the 5' end and 3' of ssDNA-1 The ends are complementary to the 3' end and 5' end of primer 1, respectively.
[0100] The nucleotide sequence of ssDNA-1 is shown in SEQ ID NO.10, see Table 1.
[0101] The nucleotide sequence of primer 1 is shown in SEQ ID NO.12, see Table 1.
[0102] Mix ssDNA-1 modified by phosphorylation at the 5' end and primer 1 at a molar ratio of 1:1.3, add NaCl at a final concentration of 80mmol / L, use sterile water to make the...
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