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Establishment method of skin-sensitized food allergy animal model

A food allergy and animal model technology, which is applied in the field of establishment of food allergy animal models, can solve problems such as inability to simulate well, and achieve the effect of simple operation, good reproducibility, good sensitization state and degree

Pending Publication Date: 2022-02-08
ZHEJIANG GONGSHANG UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The sensitization animal model of this method is gavage sensitization, which cannot well simulate the current clinical sensitization process

Method used

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  • Establishment method of skin-sensitized food allergy animal model
  • Establishment method of skin-sensitized food allergy animal model
  • Establishment method of skin-sensitized food allergy animal model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] The mouse allergy model construction scheme is as follows: figure 1 shown.

[0052] BALB / c mice were purchased from Zhejiang Academy of Medical Sciences (No. 182, Tianmushan Road, Xihu District, Hangzhou, Zhejiang Province), and were bred and operated by Zhejiang Academy of Medical Sciences. After the mice were pre-fed for 1 week, mice with a body weight of 18±1 g were used for subsequent experiments. After the mice were grouped, they were sensitized by smearing MC903 (2 nmol) ethanol solution containing OVA (100 μg) on ​​both ears every day. The smear volume was 20 μL, and the sensitization period lasted for 2 weeks. After the end of the sensitization, in the morning of the first day and noon of the fifth day after the end, the mice were challenged by gavage with a PBS solution containing OVA (50 mg), and the OVA that was gavaged was dissolved in PBS (pH=7.4) In , the concentration is 250g / L, and the gavage volume of each mouse is 200μL. The symptoms of the mice wer...

Embodiment 2

[0059] C57BL / 6 mice were purchased from Zhejiang Academy of Medical Sciences (No. 182, Tianmushan Road, Xihu District, Hangzhou, Zhejiang Province), and were bred and operated by Zhejiang Academy of Medical Sciences. After the mice were pre-fed for 1 week, mice with a body weight of 18±1 g were used for subsequent experiments. After the mice were grouped, they were sensitized by smearing MC903 (2 nmol) ethanol solution containing OVA (100 μg) on ​​both ears every day. The smear volume was 20 μL, and the sensitization period lasted for 2 weeks. After the end of the sensitization, in the morning of the first day and noon of the fifth day after the end, the mice were challenged by gavage with a PBS solution containing OVA (50 mg), and the OVA that was gavaged was dissolved in PBS (pH=7.4) In , the concentration is 250mg / mL, and the oral administration volume of each mouse is 200μL. The symptoms of the mice were recorded within 30 min after each challenge, and used for scoring al...

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Abstract

The invention discloses a method for establishing a skin-sensitized food allergy animal model, and belongs to the technical field of medical evaluation and detection. The method comprises an allergen sensitization stage and an allergen excitation stage, in the allergen sensitization stage, an MC903 ethanol solution containing OVA is smeared on the skin of an experimental animal, and in the allergen excitation stage, OVA dissolved in a buffer solution is used for intragastric administration excitation. The method of establishing the skin-sensitized food allergy animal model has the advantages of being easy and convenient to operate, short in modeling period, low in cost, good in reproducibility and the like.

Description

technical field [0001] The invention belongs to the technical field of medical evaluation and detection, and in particular relates to a method for establishing a food allergy animal model sensitized through the skin. Background technique [0002] Food allergy refers to the abnormal reaction of the body's immune system to certain food proteins. When it occurs, it can involve multiple systems in the body, such as the skin, respiratory tract, gastrointestinal tract, and central nervous system. In severe cases, it can lead to anaphylactic shock or even life-threatening. In recent years, the incidence of food allergy worldwide has been increasing year by year, and food allergy has become a public health problem of global concern, known as the "second wave" of allergic epidemics after asthma. A large number of studies have found that early skin exposure is an important risk factor for inducing food allergy. Based on this, the "double allergen exposure hypothesis" is proposed. It i...

Claims

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Application Information

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IPC IPC(8): A61K38/38A61K31/593A01K67/02
CPCA61K38/38A61K31/593A01K67/02A01K2267/0387A01K2227/105A01K2207/10A01K2207/20A61P37/08A61K39/35
Inventor 傅玲琳王彦波张巧智余刚周瑾茹
Owner ZHEJIANG GONGSHANG UNIVERSITY
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