Establishment method of skin-sensitized food allergy animal model

A food allergy and animal model technology, which is applied in the field of establishment of food allergy animal models, can solve problems such as inability to simulate well, and achieve the effect of simple operation, good reproducibility, good sensitization state and degree

Pending Publication Date: 2022-02-08
ZHEJIANG GONGSHANG UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The sensitization animal model of this method is gavage sensitization,

Method used

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  • Establishment method of skin-sensitized food allergy animal model
  • Establishment method of skin-sensitized food allergy animal model
  • Establishment method of skin-sensitized food allergy animal model

Examples

Experimental program
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Effect test

Example Embodiment

[0050] Example 1

[0051] Mouse allergy model building programs such as figure 1 Indicated.

[0052] BALB / c mice were purchased from Zhejiang Medical Sciences (Hangzhou, Zhejiang Province Lake Road Mount 182), were housed by Zhejiang Medical Sciences and related experimental operation. After the mice were preliminarily fed for 1 week, weighing 18 ± 1g mice used in subsequent experiments. After the mice were grouped by day MC903 (2nmol) ethanol solution containing a sensitizing applicator binaural OVA (100μg), the coating amount of 20 L, sensitization phase of 2 weeks. After the sensitization, respectively, in the first day after the end of the morning and afternoon fifth day, the mice were orally challenged with PBS solution containing OVA (50mg) and the gavage of OVA dissolved in PBS (pH = 7.4) a concentration of 250g / L, the amount of fed mice per 200μL. After each excitation of mice showing symptoms of the recording 30min, scoring for allergic symptoms. 24 hours after the la...

Example Embodiment

[0058] Example 2

[0059] C57BL / 6 mice were purchased from Zhejiang Medical Sciences (Hangzhou, Zhejiang Province Lake Road Mount 182), were housed by Zhejiang Medical Sciences and related experimental operation. After the mice were preliminarily fed for 1 week, weighing 18 ± 1g mice used in subsequent experiments. After the mice were grouped by day MC903 (2nmol) ethanol solution containing a sensitizing applicator binaural OVA (100μg), the coating amount of 20 L, sensitization phase of 2 weeks. After the sensitization, respectively, in the first day after the end of the morning and afternoon fifth day, the mice were orally challenged with PBS solution containing OVA (50mg) and the gavage of OVA dissolved in PBS (pH = 7.4) at a concentration of 250mg / mL, the amount of fed mice per 200μL. After each excitation of mice showing symptoms of the recording 30min, scoring for allergic symptoms. 24 hours after the last challenge, mice were bled, serum was separated for the detection o...

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Abstract

The invention discloses a method for establishing a skin-sensitized food allergy animal model, and belongs to the technical field of medical evaluation and detection. The method comprises an allergen sensitization stage and an allergen excitation stage, in the allergen sensitization stage, an MC903 ethanol solution containing OVA is smeared on the skin of an experimental animal, and in the allergen excitation stage, OVA dissolved in a buffer solution is used for intragastric administration excitation. The method of establishing the skin-sensitized food allergy animal model has the advantages of being easy and convenient to operate, short in modeling period, low in cost, good in reproducibility and the like.

Description

technical field [0001] The invention belongs to the technical field of medical evaluation and detection, and in particular relates to a method for establishing a food allergy animal model sensitized through the skin. Background technique [0002] Food allergy refers to the abnormal reaction of the body's immune system to certain food proteins. When it occurs, it can involve multiple systems in the body, such as the skin, respiratory tract, gastrointestinal tract, and central nervous system. In severe cases, it can lead to anaphylactic shock or even life-threatening. In recent years, the incidence of food allergy worldwide has been increasing year by year, and food allergy has become a public health problem of global concern, known as the "second wave" of allergic epidemics after asthma. A large number of studies have found that early skin exposure is an important risk factor for inducing food allergy. Based on this, the "double allergen exposure hypothesis" is proposed. It i...

Claims

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Application Information

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IPC IPC(8): A61K38/38A61K31/593A01K67/02
CPCA61K38/38A61K31/593A01K67/02A01K2267/0387A01K2227/105A01K2207/10A01K2207/20A61P37/08A61K39/35
Inventor 傅玲琳王彦波张巧智余刚周瑾茹
Owner ZHEJIANG GONGSHANG UNIVERSITY
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