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Orange-light carbon dot for rapidly positioning Golgi apparatus based on cyclooxygenase-2 target spot as well as preparation and application of orange-light carbon dot

A cyclooxygenase and Golgi technology, applied in nano-optics, luminescent materials, fluorescence/phosphorescence, etc., can solve the problems of inability to achieve fast and accurate Golgi imaging, limit the efficiency of fluorescence imaging, and achieve accurate Golgi targeting. Effects, high selectivity, small size effects

Pending Publication Date: 2022-02-11
TAIYUAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, in bioluminescent imaging operations, it can be found that the incubation time of existing carbon dot targeting probes is as long as 30 minutes to 4 hours, which cannot achieve fast and accurate imaging of the Golgi apparatus, which limits the efficiency of fluorescence imaging.

Method used

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  • Orange-light carbon dot for rapidly positioning Golgi apparatus based on cyclooxygenase-2 target spot as well as preparation and application of orange-light carbon dot
  • Orange-light carbon dot for rapidly positioning Golgi apparatus based on cyclooxygenase-2 target spot as well as preparation and application of orange-light carbon dot
  • Orange-light carbon dot for rapidly positioning Golgi apparatus based on cyclooxygenase-2 target spot as well as preparation and application of orange-light carbon dot

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Weigh 0.0324g of p-phenylenediamine and 0.0157g of benzenesulfonamide, add 40mL of methanol, seal with parafilm, place on an ultrasonic disperser, and ultrasonically disperse at a frequency of 60KHz for 3min to obtain a mixed solution.

[0051] The mixed solution was placed in a stainless steel reaction kettle lined with polytetrafluoroethylene, heated to 180° C. for 8 hours under sealing, and reacted for 8 hours to obtain a wine red solution product.

[0052] Filter the product with a 0.22 μm hydrophilic microporous membrane, then put the filtrate into a dialysis bag with a molecular weight cut-off of 500-1000 Da, dialyze in deionized water for 12 hours, add the dialysate into a rotary steamer, and then add 20 mL Deionized water, heated to 55°C and rotary evaporated to remove methanol, concentrated to about 15mL and stopped rotary evaporation, cooled to room temperature and placed in a -80°C refrigerator to freeze, and then frozen in a vacuum freeze-drying oven at a vac...

Embodiment 2

[0062] Weigh 0.1081g of p-phenylenediamine and 0.1572g of benzenesulfonamide, add 40mL of methanol, seal with a parafilm, place on an ultrasonic disperser, and disperse ultrasonically at a frequency of 60KHz for 5min to obtain a mixed solution.

[0063] The mixed solution was placed in a stainless steel reaction kettle lined with polytetrafluoroethylene, heated to 170° C. for 8.5 hours under sealing, and reacted for 8.5 hours to obtain a wine red solution product.

[0064]Filter the product with a 0.22 μm hydrophilic microporous membrane, then put the filtrate into a dialysis bag with a molecular weight cut-off of 500-1000 Da, dialyze in deionized water for 12 hours, add the dialysate into a rotary steamer, and then add 20 mL Deionized water, heated to 55°C and rotary evaporated to remove methanol, concentrated to about 15mL and stopped rotary evaporation, cooled to room temperature and placed in a -80°C refrigerator to freeze, and then frozen in a vacuum freeze-drying oven at ...

Embodiment 3

[0066] Weigh 0.0960g of p-phenylenediamine and 0.1410g of benzenesulfonamide, add 35mL of methanol, seal with parafilm, place on an ultrasonic disperser, and ultrasonically disperse at a frequency of 60KHz for 3min to obtain a mixed solution.

[0067] The mixed solution was placed in a stainless steel reaction kettle lined with polytetrafluoroethylene, and the temperature was raised to 180° C. for 7 hours of reaction under sealing to obtain a wine red solution product.

[0068] Filter the product with a 0.22 μm hydrophilic microporous membrane, then put the filtrate into a dialysis bag with a molecular weight cut-off of 500-1000 Da, dialyze in deionized water for 12 hours, add the dialysate into a rotary steamer, and then add 20 mL Deionized water, heated to 55°C and rotary evaporated to remove methanol, concentrated to about 15mL and stopped rotary evaporation, cooled to room temperature and placed in a -80°C refrigerator to freeze, and then frozen in a vacuum freeze-drying ov...

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Abstract

The invention relates to an orange-light carbon dot for rapidly positioning a Golgi apparatus based on a cyclooxygenase-2 target spot, which is carbon dot solid powder obtained by dissolving p-phenylenediamine and benzenesulfonamide in a solvent methanol, carrying out solvothermal reaction under a closed condition and purifying a reaction product. The orange-light carbon dot can be combined with cyclooxygenase-2 through a surface sulfonamide group, can be used as a Golgi apparatus targeted fluorescent probe, is applied to targeted imaging of the Golgi apparatus highly expressed by cyclooxygenase-2 in tumor cells, and has high selectivity and ultrafast imaging speed.

Description

technical field [0001] The invention belongs to the technical field of biological imaging, and relates to an orange-light carbon dot, in particular to an orange-light carbon dot capable of rapidly and accurately targeting the Golgi apparatus rich in cyclooxygenase-2, and the preparation of the orange-light carbon dot and apply. Background technique [0002] The Golgi apparatus is a key structure in the cell for the transport and secretion of proteins / enzymes. As an important part of cells, subtle pH, electrical or morphological changes in the Golgi apparatus can cause disorders in biological systems and lead to organ damage, such as eye, kidney and liver diseases. In addition, the Golgi apparatus produces higher levels of proteins or enzymes in inflammatory cells or tissues than in normal cells or tissues. Therefore, targeted fluorescence imaging of the Golgi apparatus is crucial for monitoring the microstructural morphology of the Golgi apparatus and imaging therapy for c...

Claims

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Application Information

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IPC IPC(8): C09K11/65B82Y20/00B82Y40/00G01N21/64
CPCC09K11/65B82Y20/00B82Y40/00G01N21/6428
Inventor 陈琳张昕李强卫迎迎杜晶磊于世平杨永珍刘旭光
Owner TAIYUAN UNIV OF TECH
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