Combined medication mode for synergistically treating human brain glioma
A technology of human glioma and drug combination, which is applied in the field of pharmacology, can solve problems such as changes in protein expression levels, and achieve a strong synergistic effect
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[0042] 1. Cell culture:
[0043] Human glioma cells were cultured in DMEM medium containing 10% calf serum in an incubator at 37°C, 5% CO2, and 90% relative humidity; Remove the culture medium, digest with 0.25% trypsin, subculture in separate bottles for 2 to 3 days, and take the cells in the logarithmic growth phase for experiments;
[0044] 2. Detection of glioma cell proliferation by MTT method:
[0045] The tumor cells in the logarithmic growth phase were digested with trypsin and made into 3×10 4 100 μL of each well of the cell suspension was added to a 96-well plate, and 100 μL of different concentrations of TMZ or PPZ and combined drugs were added to each well after 24 hours. The corresponding medium was added to the solvent control group, and 3 parallel wells were set for each concentration. After incubating at 37°C for 120 h, the supernatant was discarded, and 0.5 mg·mL was added to each well - 1 100 μL of MTT solution, cultured at 37°C for 4 hours, discarded the...
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