Method for manufacturing feedstuff protein by solid state fermentation of apple dregs liquid

A feed protein and solid-state fermentation technology, applied in animal feed, animal feed, application, etc., can solve the problems of high equipment investment and production costs, large amount of seed medium consumption, complicated pure culture of bacteria, etc., and achieve high equipment utilization rate , shorten the fermentation time and production cycle, and reduce the effect of equipment investment

Inactive Publication Date: 2005-09-07
NORTHWEST UNIV
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  • Claims
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Problems solved by technology

The method can overcome the disadvantages of the existing single solid-state fermentation to produce feed protein, such as large amount of se

Method used

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  • Method for manufacturing feedstuff protein by solid state fermentation of apple dregs liquid

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Example Embodiment

[0033] Example 1:

[0034] Seed culture medium: 200g potato, 20g sucrose, and some water; its production method is: cut the potato into small pieces, add appropriate amount of tap water and boil for 20-30min, filter, take the filtrate and add tap water to 1000mL.

[0035] Cultivation of seed strains: Take 300mL seed culture medium into a 500mL Erlenmeyer flask and sterilize it at 121℃ for 20min. After cooling to room temperature, insert each slant strain and place it in an air bath shaker at 30-32℃, 120- Cultivate at 140rpm for 36-54h to obtain mature seed strains.

[0036] The composition of the liquid submerged fermentation medium is: dry apple pomace 90%, sucrose 5%, urea 2%, (NH 4 ) 2 SO 42%, KH 2 PO 4 1%. And add 10 times the solid material of water.

[0037] The connected mixed strains and ratio: Candida utilis: Trichoderma viride: Cider yeast = 4:3:1.5,

[0038] The composition of the solid medium is: dried apple pomace 75%, bran 20%, urea 2%, (NH 4 ) 2 SO 4 2%, KH 2 PO 4 1...

Example Embodiment

[0066] Example 2:

[0067] After the fresh wet apple pomace is dried on a hot-air drying line at 65°C and crushed and sieved, the 40 mesh raw materials are taken as liquid fermentation medium and placed in a large-scale fermentation tank. The composition of the liquid fermentation medium is: dried apple pomace 90%, sucrose 4% , Urea 2%, (NH 4 ) 2 SO 4 2%, KH 2 PO 4 2%, and add 12 times the moisture of the solid material. At the same time, 8% (v / v) is connected to the seed tank to cultivate the mature mixed strains, and the ratio of the connected strains: Candida utilis: Trichoderma viride: Wine yeast: Trichoderma koningii = 2:1.5 :6:3, at 33℃, deep fermentation in liquid state for 36h, and then pour the fermentation broth into the prepared apple pomace solid medium according to the amount of 20mL / 100g solid substrate. The composition of the medium is: dried apple pomace 84% , Bran 10%, urea 3%, (NH 4 ) 2 SO 4 2%, KH 2 PO 4 1%. Under the condition of 70% water content and no steril...

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Abstract

The invention discloses a method of producing feed albumen by solid state fermentation from pomace liquid, whose productive technique is that firstly batching crushed pomance into liquid state fermentation cultivated base, then accessing mixed bacterial with germ all grown to process liquid deep fermentation, finally accessing bacterial liquid after deep fermentation into solid state fermentation cultivated base of pomace to continue fermentation. Compared with single solid state fermentation, the technique mentioned above needs less germ cultivated base, bacterial pure cultivated and reduces the cost. Meantime breed albumen product contains more albumen, nutrition with low cost, and is more fit to product in scale and spreading application for the industry of producing feed albumen by pomace fermentation.

Description

technical field [0001] The invention belongs to a method for producing feed protein by fermenting apple pomace. Background technique [0002] The so-called feed protein (Feeding protein) is single-cell protein (Single cell protein, SCP), also known as microbial protein or cell protein, refers to yeast, fungus, mold, and non-disease bacteria contained in single-celled organisms. The content of protein is as high as 45%-74%, and the combination of various amino acids is reasonable, complete in variety, rich in vitamins and trace elements, and free of cholesterol. These advantages are unmatched by general crop feed. [0003] China is the world's largest apple producer, with an annual output of more than 22 million tons, more than one-third of the world's total. Shaanxi Province is also the largest apple production base in my country. At present, the province's total apple planting area reaches 6.02 million mu, with an annual total output of more than 5.5 million tons, ranking ...

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Application Information

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IPC IPC(8): A23K10/12A23K10/37
CPCY02P60/87
Inventor 宋纪蓉徐抗震黄洁马海霞
Owner NORTHWEST UNIV
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