Diluent for blood cell analysis

A technology of blood cell analysis and blood cell analyzer, which is applied in the field of blood cell analysis, can solve the problems of unstable performance, unsafe antibacterial and preservatives, and high price, and achieve the effects of high accuracy, favorable classification and counting, and low raw material cost

Pending Publication Date: 2022-02-15
GENRUI BIOTECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The embodiment of the present invention provides a diluent for blood cell analysis, which can solve the technical problems of high price, unstable performance and unsafe antibacterial preservatives

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0025] The preparation process of the above-mentioned blood cell analysis is as figure 1 Looking:

[0026] 100, first add the above components to the beaker, dissolved with a certain amount of pure water;

[0027] 101. Second, the solution is transferred from the beaker from the beaker with a glass rod;

[0028] 102, then use the drip tube to set up to 1 liter;

[0029] 103. After the endurance is complete, it is shaken.

[0030] Adequate sodium sulfate and sodium chloride are inorganic salts, and the diluted inorganic salt solution is commonly used for blood cells to provide a human inorganic salt environment, ensuring that blood cells are in a suitable pH range, suitable osmotic pressure range and suitable Detection in the conductivity range; boric acid is a buffer, in addition to the function of buffering, because there is no phosphorus, it also avoids environmental pollution; sodium ethylenediamine is anticoagulant, which can tissue blood cell solidification; sulfate Monomethy...

Embodiment 1

[0035] Example 1: Dilution 1 for hematocyte analysis 1:

[0036] In the dilution of the blood cell analysis provided in this example, each liter of dilute is 3.00 g of sodium sulfate, 4.50 g sodium chloride, 3.00g of boric acid, 0.30 g of dice sodium ethylenediamine, dichroate 0.08 g, formaldehyde 0.50mL; specific configuration method is: first dissolve the above components, and then use pure water to 1 liter. The diluent of the pH of 7.4.

Embodiment 2

[0037] Example 2: Dilution 2 of blood cell analysis 2:

[0038] In this example, the blood cell analysis is in the dilution of the dilution, each liter of dilution comprises: 0.50 g of sodium sulfate, 5.00 g of sodium chloride, 3.00g of boric acid, 0.30 g of dice sodium ethylenediamine, nolar sulfate 0.08 g, formaldehyde 0.50mL; specific configuration method is: first dissolve the above components, and then use pure water to 1 liter. The diluent of the pH of 7.4.

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PUM

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Abstract

The invention relates to the field of blood cell analysis, in particular to a diluent for blood cell analysis. The invention discloses a diluent for blood cell analysis, and is characterized in that each liter of the diluent for blood cell analysis comprises the following components: 2.0 to 5.0 g of anhydrous sodium sulfate, 5.0 to 9.0 g of sodium chloride, 0.20 to 0.35 g of disodium ethylene diamine tetraacetate, 2.0 to 3.5 g of boric acid, 0.05 to 0.10 g of gentamicin sulfate, and 0.3 to 0.8 mL of formaldehyde. The conductivity of the diluent for blood cell analysis ranges from 15 mS/cm to 20 mS/cm, the pH ranges from 6.8 to 7.8, and the osmotic pressure ranges from 300 mOsm/kg to 330 mOsm/kg. In the formula, gentamicin sulfate and formaldehyde are used for replacing a common antibacterial preservative sodium azide, so that damage to related personnel and the environment in the production and use process is avoided; and a proper ionic strength is provided, so that a stable environment is provided for counting of blood cells, and classified counting is more facilitated.

Description

Technical field [0001] The present invention relates to the field of blood cell analysis, and more particularly to a dilution for hematocyte analysis. [0002] technical background [0003] In the field of medical clinical examination, blood cell assays have significant clinical significance for the diagnosis of patient's physical condition and the diagnosis of diseases and subsequent treatment. Blood cells were analyzed by the blood cell analyzer, and the blood cells were needed for easy analysis of cells. However, the existing blood cell analysis is generally the reagent of imported blood cell analyzer original manufacturer, which is expensive and inconvenient to transport; at present, although there is a good domestic blood cell analysis, the effect is also related to import reagents. Quite, but its price is equally high; although the price of some domestic reagents is cheap, but the stability is poor, not only the accuracy of test results cannot be guaranteed, but it may also ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/10G01N27/06
CPCG01N15/10G01N27/06G01N2015/0073G01N2015/008G01N2015/0084G01N2015/1006G01N2015/1062
Inventor 廖耀彪余洋陈明
Owner GENRUI BIOTECH INC
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