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Diluent for hematology analyzer

A blood cell analyzer and diluent technology, applied in the application, material inspection, preservation of human or animal body, etc., can solve the problems of incalculable accuracy machine damage, affecting daily use, expensive reagents, etc. Effects of mechanical damage, preservation of shape and structure, ease of preparation

Inactive Publication Date: 2013-04-03
江苏美诚生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the diluent for hematology analyzers is generally provided by the original factory of the instrument or imported reagents are packaged by domestic manufacturers. Therefore, the reagents are expensive and inconvenient to transport, which affects daily use.
Good reagents are very important to ensure the normal operation and daily maintenance of the instrument and to obtain accurate test results. Although some domestic reagents are relatively cheap, they generally have poor stability, and the accuracy of the results and the damage to the machine cannot be estimated.
The above reasons restrict the development of the blood count analysis project of the blood routine test in the laboratory department of the hospital

Method used

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  • Diluent for hematology analyzer
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Embodiment Construction

[0014] The diluent maintains the original shape of the blood cells in the blood. The main factors for maintaining the size and shape of the cells in the diluent are: molar osmotic pressure concentration, pH value, electrical conductivity, buffering force, amount of ions, ion type, deformation force, temperature etc. Changing any of these parameters will usually affect the balance of the other parameters, and at this time, the volume of blood cells will usually be affected as well. The present invention will be specifically introduced below in conjunction with specific embodiments.

[0015] First, blood cell dilutions were prepared according to the formula shown in Table 1.

[0016] Table 1 Ratio of blood cell diluent

[0017]

[0018] The preparation method is: accurately weigh NaCl, Na 2 SO 4 、Na 2 HPO 4 / NaH 2 PO 4 , EDTA-2Na, fully dissolved with 0.8L pure water, then add 1-hydroxypyridine-2-sulfur, then add formaldehyde, stir and mix well, then set the volume to...

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Abstract

The invention discloses a diluent for a hematology analyzer. The diluent for the hematology analyzer comprises sodium chloride, sodium sulfate, a phosphate buffer agent, ethylene diamine tetraacetic acid, 1-pyridone-2-sulphur and formaldehyde, and the pH value of the solution is 6.5-7.4, and preferably is 7.2; the content of each component is preferably as follows: 5g / L of sodium chloride, 10g / L of sodium sulfate, 0.3g / L of ethylene diamine tetraacetic acid, and 0.2ml / L of 1-pyridone-2-sulphur. The diluent for the hematology analyzer provided by the invention has the beneficial effects that general physical mixing can be adopted, and the preparation is extremely convenient; 1-pyridone-2-sulphur replaces the commonly used antimicrobial preservative sodium azide, is non-toxic, is safe to use, and can not cause harm or damage to reagent producers and environments; formaldehyde not only has a preservation effect, but also can keep the completeness of forms and structures of blood cells, and can increase the mechanical injury performance of the blood cells; and the diluent provided by the invention can achieve the testing effect equivalent to that of the original sheath fluid, and the cost is controlled well.

Description

technical field [0001] The invention relates to a diluent, in particular to a diluent for a blood cell analyzer. Background technique [0002] Blood cell diluent is usually a kind of isotonic solution with acid-base buffering effect, appropriate ionic strength and conductivity. Appropriate ionic strength and the same osmotic pressure as plasma can maintain the original volume of blood cells within a certain period of time; appropriate conductivity can ensure the pulse size corresponding to the cell volume. [0003] At present, the diluent for blood cell analyzers is generally provided by the original manufacturer of the instrument or subpackaged by imported reagents from domestic manufacturers. Therefore, the reagents are expensive and inconvenient to transport, which affects daily use. Good reagents are very important to ensure the normal operation and daily maintenance of the instrument and to obtain accurate test results. Although some domestic reagents are relatively ch...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/00G01N33/48
Inventor 华学山伍春宝
Owner 江苏美诚生物科技有限公司
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