Low-adhesion organoid culture chip

Abstract

The invention discloses a low-adhesion organoid culture chip which comprises a pore plate, culture holes are distributed in the inner side of a frame in a matrix manner, and the bottoms of the culture holes are U-shaped, a preparation method comprises the following steps: taking polystyrene as a raw material, adding nano silicon dioxide, mixing, heating and melting in a reaction kettle, and extruding into a mold through an extruder to obtain the pore plate; and carrying out plasma treatment on the taken-out pore plate, and forming a fluorine ion film on the surface of the pore plate. The U-shaped bottom design is adopted, and compared with a traditional round bottom, the U-shaped bottom has the advantage that cells can be automatically gathered; the culture dish is made of polystyrene and nano silicon dioxide, and both the polystyrene and the nano silicon dioxide have extremely high transparency, so that microscope observation is facilitated. And carrying out plasma treatment on the culture vessel, and controlling a surface film coating, so as to achieve the purpose of appointing the contact angle.

Description

technical field [0001] The invention belongs to the technical field of organoid model culture, in particular to a low-adhesion organoid culture chip. Background technique [0002] In recent years, an in vitro three-dimensional cell culture system called "organoid" has opened up new opportunities for preclinical personalized therapy and oncology drug testing. This three-dimensional culture system has a high degree of similarity with tissues or organs derived from in vivo tissues. Compared with traditional two-dimensional cell culture models, organoids have stable characterization and genetic characteristics in terms of physical, molecular and physiological characteristics, and can be cultured in vitro for a long time. Can be closer to the real situation of human tumors than cell lines. Organoids better reproduce the differentiation capacity of tissue-specific lineages and the self-renewal of stem cells. Compared with xenograft models, organoid culture has a short establish...

AI Technical Summary

Problems solved by technology

[0003] In the culture plate of existing organoids, the bottom of the culture well is a round bottom, and there is a contact angle between the bottom and the side wall of the culture well. The culture medium and cells are easy to deposit and adsorb on the inner wall of the culture well. Angle observation has micropores invisible to the naked eye, which have retention and adsorption effects on liquid and cell tissue, and it is difficult for organoid cells to form clusters

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