Preparation method of reduced nicotinamide adenine dinucleotide

Abstract

The invention relates to the technical field of industrial catalysis, in particular to a preparation method of reduced nicotinamide adenine dinucleotide, which comprises the following steps: 1) screening the activity of formate dehydrogenase at high temperature; 2) screening the activity of formate dehydrogenase under an acidic condition; (3) according to the results of the step (1) and the step (2), formate dehydrogenase with relatively high activity under a high-temperature condition and an acidic condition is selected for later use; respectively weighing ammonium formate and NAD (Nicotinamide Adenine Dinucleotide) into a flask, adding pure water, stirring and dissolving in a water bath, and regulating the pH to be acidic by using sodium hydroxide; finally, formate dehydrogenase obtained through screening is added for reaction, sodium hydroxide is used for maintaining the pH value to be acidic, and the reduced nicotinamide adenine dinucleotide is prepared. According to the method, the reaction can be completed in a short time, so that hydrolysis of a product under an acidic condition is avoided, and carbonate generated under an alkaline condition is also avoided; after the reaction, no large amount of carbonate exists, and the purification cost is low.

Description

technical field [0001] The invention relates to the technical field of industrial catalysis, specifically a method for preparing reduced nicotinamide adenine dinucleotide. Background technique [0002] Reduced nicotinamide adenine dinucleotide (NADH) is a coenzyme of various redox enzymes, which plays an important role in industrial catalysis. In recent years, researchers have found that NADH also has a variety of human health functions, which may be a potential protective Health products / drugs for liver protection and hangover. [0003] The mainstream production method of NADH is prepared by extracting NAD from yeast and reducing it to NADH. At present, there are many enzymatic methods for reducing NAD to NADH, such as alcohol dehydrogenase, formate dehydrogenase, glucose dehydrogenase and 6-phosphate glucose dehydrogenase, etc., but different preparation processes affect the purification of the product larger. [0004] Currently, the production of NADH mainly uses alcoh...

AI Technical Summary

Problems solved by technology

However, the reduction of NAD by formate dehydrogenase will generate carbon dioxide, which escapes from the reaction mixture only at acidic to neutral pH, and the easy formation of carbonate in alkaline solution is not conducive to post-treatment purification

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