Automatic bacterium detection system and method based on digital microfluidics

Abstract

The invention provides an automatic bacteria detection system and method based on digital microfluidics, and the system comprises a digital microfluidic chip which is used for separating and enriching bacteria in a sample; the light path acquisition module is arranged right above the digital micro-fluidic chip and is used for acquiring light signals; the magnetic field control module is used for controlling activation and closing of a magnetic field when the magnetic immune organisms emit light; the signal conditioning module is used for conditioning the optical signals acquired by the optical path acquisition module; and the chip control circuit is respectively connected with the digital micro-fluidic chip and the light path acquisition module. According to the present invention, by using the digital microfluidic technology, the bacteria are rapidly and automatically detected, the liquid drop generation, the splitting and the transportation are completed on the basis of the control to achieve the movement of the single or the plurality of the discrete liquid drops on the chip plane, and the advantages of miniaturization, integration, automation, parallelization, high efficiency and low cost are provided.

Description

technical field [0001] The invention relates to the technical field of digital microfluidics, in particular to an automatic bacteria detection system and method based on digital microfluidics. Background technique [0002] The statements in this section merely provide background information related to the present invention and do not necessarily constitute prior art. [0003] Pathogens, chemical contamination, and illegal food additives are the three major sources of food safety problems, with pathogen contamination caused by pathogenic bacteria being the primary concern. The most effective means of reducing the threat of pathogens is the timely and accurate detection of pathogens in food. The current detection method stipulated in the national standard is the traditional culture method. The detection result of this method is accurate and the cost is relatively cheap. However, the detection time is 24-48 hours, which is time-consuming and laborious. Obviously, it cannot mee...

AI Technical Summary

Problems solved by technology

[0006] However, there is no research on the use of digital microfluidic technology for automatic detection to isolate and enrich bacteria in samples. Therefore, there is an urgent need for an automated bacterial detection system and method based on digital microfluidics.

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