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Process for enriching high-purity catechin

A catechin, high-purity technology, applied in the direction of organic chemistry and the like, can solve the problems of difficult regeneration of the adsorbent, complicated operation, and difficult to industrialize production, and achieve the effects of easy industrial control, simple operation, and low cost.

Inactive Publication Date: 2004-03-10
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Other methods such as column separation preparation, membrane separation preparation, and droplet countercurrent distribution method can obtain tea polyphenols products with high purity catechins, but due to their large investment, low yield, difficult regeneration of adsorbent and difficult operation The disadvantages such as complexity make it only in the laboratory for a small amount of preparation and not easy for industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Weigh 1g of tea extract with a total amount of tea polyphenols greater than 95%, add 2ml of methanol or other dissolving solvents to dissolve it (if there is any insoluble part, filter it out), and obtain a reddish-brown solution (do not heat). Then, slowly add 10ml of dichloromethane or other crystallization solvents at a low temperature of 4-6°C, wait until the precipitation is complete, stir to disperse the agglomerates, let stand for 30min, fill with nitrogen and vacuum filter to obtain a filter residue, and discard the filtrate. Take the filter residue and add an equal volume of methanol or other dissolving solvents to dissolve it, add an equal volume of dichloromethane or other crystallization solvents at a low temperature of 4-6°C to precipitate, fill with nitrogen and vacuum filter to obtain the filter residue. This was repeated no less than 3 times, and the filter residue was dissolved in distilled water and then freeze-dried to dryness to obtain straw yellow po...

Embodiment 2

[0011] Weigh 25g of tea extract whose total amount of tea polyphenols is greater than 90%, add 75ml of acetone or other dissolving solvent to dissolve it (no insoluble matter), add 450ml of chloroform or other crystals to this solution at a low temperature of 4-6°C Solvent, stir to precipitate catechin, let it stand for 30 minutes, vacuum filter with nitrogen to obtain filter cake; dissolve it with 75ml of acetone or other dissolving solvent (slightly insoluble matter is removed by filtration), add 450ml of Chloroform or other crystallization solvents make the catechins separate out, vacuum suction filtration with nitrogen filling, get the filter cake, repeat like this 4 times, freeze-dry after dissolving with distilled water to get 10.2 grams of straw yellow powder, analyze the total content of catechins through HPLC as 95.2%.

Embodiment 3

[0013] Weigh 50 grams of tea extract with a total amount of tea polyphenols greater than 80%, add 200ml of ether or other dissolving solvents to dissolve it, remove insoluble matter, add 1400ml of dichloromethane or other crystals to this solution at a low temperature of 4-6°C Solvent, stir to precipitate catechin, let stand for 30min, vacuum filter with nitrogen to get filter cake; add 200ml of ether or other dissolving solvent to dissolve it, add 1400ml of dichloromethane or For other crystallization solvents, stir to precipitate the catechins, let stand for 30 minutes, and vacuum filter with nitrogen to obtain a filter cake. Repeat this 5 times, dissolve in distilled water, freeze-dry to obtain 14.2 grams of straw yellow powder, and analyze the total content of catechins by HPLC was 96.1%.

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Abstract

A process for enriching high-purity catechin includes such steps as dissolving tea extract in solvent, filtering, slowly adding crystallizing solvent at 4-6 deg.C until the catechin is fully separated out, stirring, standing, filling nitrogen gas, vacuum filtering, dissolving the dregs in same solvent, precipitation, filling nitrogen gas, repeating same steps at least 3 times, dissolving filter residue in distilled water, and freeze drying. Its advantages are high yield and low cost.

Description

technical field [0001] The invention relates to a method for enriching high-purity catechins. Background technique [0002] Tea polyphenols (Tea polyphenols, referred to as TP) is a natural antioxidant extracted from the traditional beverage green tea, which is composed of flavanols (catechins), anthocyanins, flavonoids and flavonols, A complex composed of more than 30 compounds in four categories, including phenolic acids and phenolic acids. It has a variety of health care and pharmacological effects such as scavenging free radicals, anti-oxidation, anti-mutation, anti-aging, anti-radiation, anti-cancer and anti-cancer, lowering blood fat and cholesterol, anti-atherosclerosis and antibacterial. It has been proved that the antioxidant effect of tea polyphenols is closely related to the effect of its main component, catechin. Therefore, the research and development of a new process for the production of high-purity catechin products is of great sig...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/62
Inventor 沈生荣于海宁
Owner ZHEJIANG UNIV
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