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Latex microsphere immunodetection test strip, kit and method

A technology of latex microspheres and immunoassays, which is applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of short detection time limit, complicated operation, and long detection time, and achieve the effect of improving efficiency

Pending Publication Date: 2022-03-04
佛山墨赛生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Based on this, it is necessary to provide a latex microsphere immunoassay test paper capable of rapid detection, high sensitivity and good specificity for the problems of short detection time limit, long detection time and complicated operation of phencyclidine and oxycodone Strip, test kit and drug detection method using the latex microsphere immunodetection test strip

Method used

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  • Latex microsphere immunodetection test strip, kit and method
  • Latex microsphere immunodetection test strip, kit and method
  • Latex microsphere immunodetection test strip, kit and method

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preparation example Construction

[0069] The embodiment of the second aspect of the present invention provides a kind of preparation method as above-mentioned latex microsphere immunoassay test strip, comprises the steps:

[0070] Prepare the binding pad and the detection membrane respectively, wherein,

[0071] The step of preparing the binding pad 2 includes: preparing a solution containing a specific monoclonal antibody labeled with latex microspheres, spraying the prepared latex microsphere-labeled specific monoclonal antibody on the binding pad 2, placing the binding pad 2 on the Dry in a blast drying oven at 37°C to 45°C for 1h to 7h;

[0072] The steps of preparing the detection membrane 3 include: preparing and obtaining a solution containing a synthetic antigen of oxycodone, a solution containing a synthetic antigen of phencyclidine, and a solution containing a quality control protein, respectively marking the detection membrane 3, and placing the detection membrane 3 on the Dry in a blast drying ove...

Embodiment 1

[0106] Embodiment 1: latex microsphere immunoassay test strip

[0107] Such as figure 1 As shown, the latex microsphere immunoassay test strip of the present embodiment includes a test strip body, and the test strip body includes a base plate 5 and a sample pad 1, which is arranged on the base plate 5 and is sequentially connected from one end of the base plate 5 to the other end. Combine pad 2, detection membrane 3 and absorbent pad 4. Wherein, the assembly sequence of the latex microsphere immunoassay test strip is as follows: the detection film 3 is fixed on the base plate 5, one end of the absorption pad 4 is connected with the base plate 5, the other end of the absorption pad 4 is connected with the detection film 3, and the binding pad 2 One end is connected to the end of the detection film 3 away from the absorbent pad 4 , the other end of the binding pad 2 is set on the bottom plate 5 , one end of the sample pad 1 is connected to the binding pad 2 , and the other end ...

Embodiment 2

[0111] Embodiment 2: Preparation of latex microsphere immunoassay kit

[0112] The latex microsphere immunoassay kit of the present embodiment includes a housing and the above-mentioned latex microsphere immunoassay test strip, sample lysate and detection card, the latex microsphere immunoassay test strip is arranged in the test card, and the test card A sample slot and an observation window are provided. The sample pad 1 is located in the sample slot of the test card, and the observation window corresponds to the detection line (T1) 7 and detection line (T2) on the detection film 3 of the latex microsphere immunoassay test strip. 8 and quality control line 6. The sample lysate was aliquoted into 2mL cryovials for storage.

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Abstract

The invention relates to a latex microsphere immunodetection test strip, a kit and a method. The latex microsphere immunodetection test strip comprises a sample pad, a combination pad, a detection membrane, an absorption pad and a bottom plate, wherein the sample pad, the combination pad, the detection membrane and the absorption pad are sequentially connected from one end to the other end of the bottom plate; the combination pad is coated with a specific monoclonal antibody marked by latex microspheres, the specific monoclonal antibody marked by the latex microspheres can react with drugs, and the drugs comprise phencyclidine and oxycodone; a detection line and a quality control line formed by quality control protein are arranged on the detection film, and the detection line is coated with synthetic antigens of corresponding drugs. The latex microsphere immunodetection test strip can rapidly detect oxycodone and phencyclidine in hair, is high in sensitivity and good in specificity, does not need a detection instrument, and is convenient to operate.

Description

technical field [0001] The invention relates to the technical field of drug detection, in particular to a test strip, a kit and a method for latex microsphere immunological detection of oxycodone and phencyclidine in hair. Background technique [0002] Phencyclidine is a hallucinogenic drug with narcotic effects. Phencyclidine exerts multiple effects on the human body by affecting the activity of the human brain and central nervous system. Taking large doses of phencyclidine will produce acute poisoning symptoms such as hallucinations, convulsions, coma and even death. 1 to 2 hours after taking large doses of drugs, drug addicts begin to experience emotional instability, agitation, loss of pain, nerve numbness, and self-feeling of weightlessness. Aggressive or self-harming behavior. [0003] Oxycodone is an alkaloid extracted from the drug thebaine. It is a kind of opioid drug. Its composition is similar to morphine, and it acts on the human body as an inhibitory drug. I...

Claims

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Application Information

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IPC IPC(8): G01N33/94G01N33/577G01N33/558G01N33/58
CPCG01N33/94G01N33/9486G01N33/577G01N33/558G01N33/587
Inventor 谭有将漆彦斌梁德智江海婷黄子珊张国芬
Owner 佛山墨赛生物技术有限公司
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