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Application of 4-hydroxyphenylacetic acid as marker in preparation of diagnostic kit for sepsis encephalopathy

A technology of hydroxyphenylacetic acid and a kit, which is applied in the medical field and can solve problems affecting the early diagnosis and treatment of septic encephalopathy

Pending Publication Date: 2022-03-15
CHONGQING MEDICAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although relevant studies have shown that dynamic EEG, assessment of the Glasgow Coma Scale (GCS score) and cranial magnetic resonance (MRI) can evaluate and judge the damage to the nervous system of patients, severe patients in the intensive care unit often require sedation Analgesia, mechanical assisted ventilation and other treatments have affected the early diagnosis and treatment of septic encephalopathy to a certain extent.

Method used

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  • Application of 4-hydroxyphenylacetic acid as marker in preparation of diagnostic kit for sepsis encephalopathy
  • Application of 4-hydroxyphenylacetic acid as marker in preparation of diagnostic kit for sepsis encephalopathy
  • Application of 4-hydroxyphenylacetic acid as marker in preparation of diagnostic kit for sepsis encephalopathy

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Choose from October to February 2015, in February 2016, 31 patients with the first hospital of Chongqing Medical University, selected 23 cases of medical examination centers of the First Affiliated Hospital of Chongqing Medical University, and adopting the valerized serum specimens to detect the gas chromatography method. Pests with sepsis and healthy serum metabolism. And use T test for statistical analysis of metabolites, and screening 124 metabolites has a difference.

[0032] Test results figure 1 . The gas chromatography was detected 222 kinds of metabolites, of which 124 in the medium metabolite has statistically significant, and results showed that 4-hydroxybenzoic acid increased significantly in sepsis patients.

[0033] In summary, it is found that 4-hydroxybenzoic acid can be used as a marker to take a sign of patients and healthy people in 4-hydroxybenzoic acid compared to healthy volunteers. .

Embodiment 2

[0035] According to the related clinical indicators of the collected 31 sepsis patients, the patients who followed severe ventroids were taken within 1 hour of the hospital for 1 hour, and the degree of consciousness of patient consciousness was classified according to GCS scores. Where GCS = 15 is divided into group 1, GCS is 14 to 12 as group 2, GCS is 11 to 9 as group 3, and GCS is 8 to 3 as group 4. according to Figure 2-3 It can be seen that the serum metabolites are statistically analyzed by ANOVA testing: the results of the test: 4 metabolites in the experiment have high statistical differences in 4 groups, of which 4-hydroxybenzoic acid is in group 1, group 2, group 3 is significantly increased in group 4, and there is a significant decrease in group 4.

[0036] In summary, 4-hydroxybenzoic acid is different from the degree of consciousness in serum and the degree of consciousness of patients, and the degree of consciousness of patient consciousness can be assessed by quan...

Embodiment 3

[0038] This study uses the principle of bromination reaction, through BR 2 The ACOH solvent soaked glass fiber paper and the serum 4-hydroxyphenylacetic acid can have a bromination reaction at room temperature, and the reaction time is 5 minutes, there are three kinds of color of methyl red, bromide green, and extortion. The agent is developed.

[0039] See Figure 4The present invention provides the following technical solution: a serum 4-hydroxy-phenylacetic acid detection test cartridge, including the casing 1, the cover 2, the detection belt 3; the casing 1 and the cartridge cover 2 are connected to each other by the shaft 4, the box 1 inside The arrangement is provided with the tube groove 5, the detection belt 6 is provided, and the reagent tank 7 is provided on the casing 2, the observation groove 8.

[0040] The working principle and usage process of the present invention: When used, the detecting person can add the serum sample after centrifugation to the reagent tank 7 by...

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Abstract

The invention relates to the technical field of medicine, in particular to application of 4-hydroxyphenylacetic acid as a marker in preparation of a kit for diagnosis, disease degree evaluation and / or prognosis evaluation of sepsis encephalopathy and related diseases thereof. According to the invention, it is found for the first time that 4-hydroxyphenylacetic acid is related to occurrence and development of sepsis encephalopathy, diagnosis, disease degree evaluation and prognosis evaluation can be carried out on sepsis encephalopathy through quantitative detection of 4-hydroxyphenylacetic acid, detection objects are serum, whole blood or blood samples, and the detection method is simple and easy to operate and has good application prospects.

Description

Technical field [0001] The present invention relates to the field of medical technology, and more particularly to 4-hydroxybenzoic acid as a marker in the diagnostic kit for preparing sepsis encephalopathy. Background technique [0002] Sepsis is a multi-organ dysfunction caused by a systemic inflammatory response caused by infection. It is a common fattened disease in the intensive care chamber, with high incidence (30-70%) and mortality (16-65%). Features. Sepsis can cause multi-organ functional disorders, while sepsis associatendencephalopathy, SAE is one of the complications of severe sepsis, refers to the evidence of no exact infection in the nervous system. A diffuse brain dysfunction syndrome caused by systemic inflammation. However, the pathogenesis of sepsis is complex. The specific pathogenesis is still unclear. At present, the current pathophysiological mechanism is neurotransmitter and abnormal amino acid, cerebroic flow changes and blood microcirculation disorders, i...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N21/76G01N21/78G01N33/53
CPCG01N30/02G01N21/76G01N21/78G01N33/5308G01N2800/52G01N2800/28G01N2800/26
Inventor 林时辉祝静张苜柴雨森徐昉赵乙汜罗仁杰
Owner CHONGQING MEDICAL UNIVERSITY
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