Temperature-sensitive gel material composition as well as preparation method and application thereof

A temperature-sensitive gel and gel technology, applied in the field of regenerative medicine, can solve the problems of increased cost and high requirements of 3D printing technology, and achieve the effect of promoting regeneration

Active Publication Date: 2022-03-18
THE FIRST AFFILIATED HOSPITAL OF JINAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in order to ensure the adaptability of defects in different situations, the stent needs to be precisely designed according to the actual situation, which will increase additional costs; moreover, the complicated stent manufacturing process and often requires invasive stent implantation, making 3D printing high technical requirements

Method used

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  • Temperature-sensitive gel material composition as well as preparation method and application thereof
  • Temperature-sensitive gel material composition as well as preparation method and application thereof
  • Temperature-sensitive gel material composition as well as preparation method and application thereof

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preparation example Construction

[0042] The present invention also provides a method for preparing a temperature-sensitive gel material combination, comprising the following steps:

[0043] (1) chitosan is mixed with acetic acid solution to obtain chitosan solution;

[0044] (2) dissolving β-glycerophosphate disodium and hyaluronic acid in water to obtain a hyaluronic acid solution;

[0045] (3) chitosan solution is mixed with hyaluronic acid solution to obtain chitosan hyaluronic acid solution;

[0046] (4) mixing SDF-1 with 40-60% volume chitosan hyaluronic acid solution to obtain a chitosan hyaluronic acid solution loaded with SDF-1;

[0047] (5) Mix 40-60% volume chitosan hyaluronic acid solution with 1-ethyl-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide, then add Apt19S aptamer, Apt19S-loaded chitosan hyaluronic acid solution was obtained.

[0048] The invention mixes chitosan with acetic acid solution to obtain chitosan solution.

[0049] In the present invention, the concentration of ...

Embodiment 1

[0092] Dissolve 48mg of chitosan (CS) in 1.6mL of 0.1M acetic acid solution, place it on a magnetic stirrer for 2h (1000rpm), and sterilize it by high-pressure steam at 121°C for 10min to form the chitosan solution. 240 mg of β-GP and 4 mg of hyaluronic acid were dissolved in 0.4 mL of deionized water, and sterilized by filtration with a 0.22 μm filter membrane to obtain a hyaluronic acid solution. Put chitosan solution and hyaluronic acid solution in ice bath (0°C) for 15min, then add hyaluronic acid dropwise into chitosan solution and stir with magnetic stirrer for 10min (1000rpm) to obtain chitosan hyaluronic acid solution . The chitosan hyaluronic acid solution was equally divided into two parts, each 1 mL.

[0093] Take 1mL chitosan-hyaluronic acid solution, add 1μl of SDF-1 solution with a concentration of 100μg / mL dissolved in PBS dropwise, and magnetically stir for 10min (1000rpm) to obtain a loaded SDF with a SDF-1 concentration of 200ng / mL. -1 chitosan hyaluronic a...

Embodiment 2

[0098] 100 μL of the third-generation rBMSCs culture solution (containing 1 × 10 3 cells) were inoculated into the upper chamber of the Transwell chamber, and 600 μL of α-MEM medium containing 200 ng / mL SDF-1 and 600 μL of α-MEM medium containing amino-modified Apt19S at a concentration of 2 nmol / mL were added to the lower chamber of the Transwell. at 37°C, CO 2 After culturing in an incubator with a concentration of 5% for 24 hours, wipe off the non-migrated cells in the upper chamber with a cotton swab, and fix the cells that migrated to the lower chamber with 4% formaldehyde for 15 minutes, wash with PBS twice, and dry them in the air, then 0.5% crystal violet for 30 minutes, Rinse with PBS 3 times. Then 5 fields of view were randomly selected in each small chamber under the microscope, and the BMSC cells migrating to the lower chamber were counted. The results showed that the addition of SDF-1 and amino-modified Apt19S aptamers could significantly promote the migration o...

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Abstract

The invention provides a temperature-sensitive hydrogel material composition as well as a preparation method and application thereof, and belongs to the technical field of regenerative medicine. The temperature-sensitive gel material composition provided by the invention comprises a chitosan hyaluronic acid solution loaded with SDF-1 and a chitosan hyaluronic acid solution loaded with Apt19S. The invention aims to develop a temperature-sensitive injectable double-layer hydrogel loaded with related biological factors capable of promoting cell migration, and the hydrogel is used for promoting regeneration of periodontal tissues.

Description

technical field [0001] The invention relates to the technical field of regenerative medicine, in particular to a combination of temperature-sensitive gel materials and its preparation method and application. Background technique [0002] Chronic periodontitis is a common clinical chronic inflammatory disease with progressive destruction of periodontal supporting tissue as the main pathological change, which can lead to tooth loosening or even loss in severe cases. The existing treatment methods such as supragingival scaling and subgingival scaling can only control the progress of the disease, but cannot rebuild the destroyed periodontal tissue. Therefore, many scholars expect to use tissue engineering to achieve periodontal tissue regeneration and provide a new idea for the treatment of chronic periodontitis. [0003] As one of the three elements of tissue engineering, seed cells are the basic conditions for tissue regeneration. However, the method of culturing and implant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/22A61L27/54A61L27/52A61L27/50
CPCA61L27/20A61L27/227A61L27/54A61L27/52A61L27/50A61L2430/12A61L2430/34A61L2300/252A61L2300/412A61L2400/06C08L5/08C08L89/00A61L2300/414A61L27/48A61L27/56
Inventor 刘湘宁蔡明详许梓楠梁言
Owner THE FIRST AFFILIATED HOSPITAL OF JINAN UNIV
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