Exosome separation and enrichment method based on exosome enrichment chip
A technology for separating and enriching exosomes, applied in chemical instruments and methods, biochemical equipment and methods, and laboratory containers, etc., can solve the problems of large consumption and cumbersome and complicated operation steps, and achieve the promotion of mixing and simplification Manual operation, the effect of reducing errors
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Embodiment 1
[0044] An exosome enrichment chip based on the principle of chitosan positive and negative charge adsorption, such as figure 1 As shown, the exosome enrichment chip based on the chitosan positive and negative charge adsorption principle is composed of upper and lower two-layer chips, wherein: the upper layer is a straight channel liquid layer ( figure 2 ), and the lower layer is the triangular prism mixed flow layer ( image 3 );
[0045] The straight channel liquid path layer is provided with the following structure:
[0046] ——Sampling port (1): located at the most upstream of the liquid path layer of the entire straight channel;
[0047] - Straight channel area (3): Arranged between the inlet and the outlet for communication between the two;
[0048]——Sample outlet (2): located at the most downstream of the liquid path layer of the entire straight channel;
[0049] The triangular prism mixed flow liquid path layer is located directly below the straight channel liquid p...
Embodiment 2
[0066] Isolation and enrichment of exosomes from blood samples
[0067] The microfluidic chip prepared in Example 1 was selected to separate and enrich the exosomes in the blood sample, and the specific steps were as follows:
[0068] (1) Take out 10 μL of the sample to be separated, and mix it with MES (2-(N-morpholino)ethanesulfonic acid) buffer solution with a pH of 5 according to the sample: buffer solution = 1:4, and set aside;
[0069] (2) Use a syringe to draw the sample to be separated, and use a circulating syringe pump to inject the sample from the sample inlet into the chip, insert a yellow tip of more than 100 μL at the sample outlet to prevent the sample from overflowing, and set the cycle injection according to the volume of the sample to be separated The pump rate is 1 cycle per minute for a duration of 20 minutes;
[0070] (3) Use MES to rinse the channel twice to remove excess samples after the injection;
[0071] (4) Use a vacuum pump to remove excess liqui...
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