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Antibacterial functional polypeptide as well as preparation method and application thereof

A multi-functional and antibacterial peptide technology, applied in the field of antibacterial functional peptides and their preparation, can solve the problems of cumbersome operation steps, low precision, inaccurate comparison data, etc., and achieve the effect of simple preparation method

Pending Publication Date: 2022-03-18
江苏申琅生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies of the prior art, the present invention provides an antibacterial functional polypeptide and its preparation method and application, which solves the above-mentioned background art and proposes that the existing drug preparation generally extracts immune genes produced by plants or animals, or the immune genes produced in animal cells. The preparation of peptides, the operation steps are cumbersome, the precision is low, and the comparison data is inaccurate.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0023] The invention provides a technical solution: an antibacterial functional polypeptide and its preparation method and application, including an antibacterial peptide with two or more disulfide bonds and a β-sheet structure.

[0024] The preparation method of the antibacterial functional polypeptide comprises the following steps:

[0025] S1. Cultivate colonies: place bovine neutrophils in a petri dish, add yeast extract, tryptone, D-glucose, cultivate, take bovine neutrophil culture fluid and transfer to cell culture medium to continue culturing, Until a suitable period of time, then, put the culture medium into a cell centrifuge, centrifuge, and collect the bacteria;

[0026] S2. Plasmid preparation solution: Take the above step S1 and pour the culture solution in the culture colony into the experimental shaker, filter out the sediment carried by the shaker solution, and add cold ethanol to the supernatant of the centrifuge tube, slowly Rinse the inner wall of the centr...

Embodiment example 2

[0037] The invention provides a technical solution: an antibacterial functional polypeptide and its preparation method and application, including an antibacterial peptide with two or more disulfide bonds and a β-sheet structure.

[0038] The preparation method of the antibacterial functional polypeptide comprises the following steps:

[0039] S1. Cultivate colonies: place bovine neutrophils in a petri dish, add yeast extract, tryptone, D-glucose, cultivate, take bovine neutrophil culture fluid and transfer to cell culture medium to continue culturing, Until a suitable period of time, then, put the culture medium into a cell centrifuge, centrifuge, and collect the bacteria;

[0040] S2. Plasmid preparation solution: Take the above step S1 and pour the culture solution in the culture colony into the experimental shaker, filter out the sediment carried by the shaker solution, and add cold ethanol to the supernatant of the centrifuge tube, slowly Rinse the inner wall of the centr...

Embodiment example 3

[0051] The invention provides a technical solution: an antibacterial functional polypeptide and its preparation method and application, including an antibacterial peptide with two or more disulfide bonds and a β-sheet structure.

[0052] The preparation method of the antibacterial functional polypeptide comprises the following steps:

[0053] S1. Cultivate colonies: place bovine neutrophils in a petri dish, add yeast extract, tryptone, D-glucose, cultivate, take bovine neutrophil culture fluid and transfer to cell culture medium to continue culturing, Until a suitable period of time, then, put the culture medium into a cell centrifuge, centrifuge, and collect the bacteria;

[0054] S2. Plasmid preparation solution: Take the above step S1 and pour the culture solution in the culture colony into the experimental shaker, filter out the sediment carried by the shaker solution, and add cold ethanol to the supernatant of the centrifuge tube, slowly Rinse the inner wall of the centr...

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Abstract

The invention discloses an antibacterial functional polypeptide as well as a preparation method and application thereof, and relates to the technical field of preparation methods and application of antibacterial functional polypeptides, in particular to an antibacterial functional polypeptide as well as a preparation method and application thereof, the antibacterial functional polypeptide comprises an antibacterial peptide with two or more disulfide bonds and a beta-folding structure; the preparation method of the antibacterial functional polypeptide comprises the following steps: S1, culturing bacterial colonies; s2, preparing a plasmid preparation solution; s3, carrying out double enzyme digestion detection on the extracted recombinant plasmids; the polypeptide with the antibacterial function is applied to preparation of antibacterial drugs. According to the antibacterial functional polypeptide and the preparation method and application thereof, the preparation method of the antibacterial functional polypeptide is simple, many contrast tests are involved, and contrast test comparison data under the same condition are obtained, so that the beneficial effects generated by different contents of the antibacterial functional polypeptide under the comparison condition are judged; and the antibacterial functional polypeptides with different contents can be prepared into medicines, so that different application ranges can be adjusted.

Description

technical field [0001] The invention relates to the technical field of antibacterial functional polypeptides, in particular to an antibacterial functional polypeptide and its preparation method and application. Background technique [0002] Antimicrobial peptide refers to a class of basic polypeptide substances with antibacterial activity induced by insects, with a molecular weight of about 2000-7000 and composed of 20-60 amino acid residues. Most of these active peptides have the characteristics of strong alkalinity, thermal stability and broad-spectrum antibacterial properties. The first antimicrobial peptide discovered in the world was a polypeptide with antibacterial activity produced by the chrysalis of Cecropins chrysalis in 1980 by Swedish scientist G.Boman et al. through injection of Bacillus vaginalis and Escherichia coli, named Cecropins. [0003] Antimicrobial peptides can be divided into various types according to their structures, among which Cathelicidin and D...

Claims

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Application Information

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IPC IPC(8): C12N5/0787C12N15/10C07K1/08A61P31/00
CPCC12N5/0642C12N15/1003C07K1/08A61P31/00
Inventor 胡勇
Owner 江苏申琅生物科技有限公司
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