Activated partial thromboplastin generation combined correction test and detection kit

Abstract

The invention relates to a method for simultaneously detecting five hemorrhagic diseases clinically and a preparation method of a kit. The method is characterized by comprising the following steps: (1) preparing a kit containing fresh mixed plasma, barium sulfate adsorbed plasma and stored serum; and (2) adding a certain amount of plasma of a patient to be detected into each tube of the kit, and respectively detecting activated partial thromboplastin time (APTT) of each tube. The five hemorrhagic diseases are diagnosed in a laboratory according to the comparison condition (correction condition) of the APTT time of the reagent containing tube and the APTT time of the plasma of the patient. And (3) diluting the mixed plasma with different concentrations by using the stored serum and the barium sulfate adsorbed plasma respectively, determining activated partial thromboplastin time (APTT) to obtain standard curves of factors VIII and IX, and quantitatively determining the factors VIII and IX of the patient.

Description

technical field [0001] The invention relates to a test method and a test kit for in vitro diagnosis of hemorrhagic diseases. Background technique [0002] At present, various clinical bleeding disorders require different testing methods for screening, exclusion, and diagnosis, and multiple blood collection tests are required, which is troublesome, time-consuming, and costly. The method uses a kit for one person and uses a hemagglutination instrument to carry out laboratory diagnosis of five bleeding diseases, which avoids the disadvantages of multiple blood collections for a single test, prolonged diagnosis time, and high price. The sensitivity and accuracy of this method are high. [0003] The method of the present invention draws lessons from the inspection methods before the 1990s: "Serum Prothrombin Generation and Correction Test", "Simple Thromboplastin Generation and Correction Test". The reagent production and detection methods of this method are all manually operate...

AI Technical Summary

Problems solved by technology

[0003] The method of the present invention draws lessons from the inspection methods before the 1990s: "Serum Prothrombin Generation and Correction Test", "Simple Thromboplastin Generation and Correction Test". The reagent production and detection methods of this method are all manually operated, and the process is cumbersome. , standards are not uniform, sensitivity and accuracy are low, and the application has long been discontinued