A kind of method and application of controlling Spodoptera frugiperda by using sex pheromone fascination agent
A technology of Spodoptera frugiperda and pheromone, which is applied in the field of biological control, can solve the problems of ecological environment damage to food quality and safety, affect the survival of natural enemies, and reduce the control effect, and achieve the effects of environmental friendliness, long-lasting effect and simple operation
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Embodiment 1
[0012] Embodiment 1 The preparation method of Spodoptera frugiperda sex pheromone fascination agent
[0013] A polyvinyl chloride capillary (PVC) (length 20cm x inner diameter 1.5mm) with slow release effect was selected as the carrier. The corn oil solution containing cis-8-tetradecene acetate was injected into a polyvinyl chloride capillary in a dose of 5-15 mg, and 2% of 2,6-di-tert-butyl-4-methylphenol ( BHT) as a stabilizer, after the completion, the two ends of the capillary are heated and sealed to make the Spodoptera frugiperda sex pheromone fascination agent lure core. Before the test, the lure core was placed in a -20 ℃ refrigerator and stored for later use.
Embodiment 2
[0014] Example 2 Field Biological Activity Determination Test
[0015] The lure core prepared in Example 1 was selected and hung in the corn field where the damage of Spodoptera frugiperda was relatively serious for testing. Three replicates were set for each of the above lure treatments. Table 1 shows the different doses, placement numbers and inhibition of mating rates.
[0016]
[0017]
[0018] The test results showed that cis-8-tetradecene acetate had different degrees of inhibition on the mating of Spodoptera frugiperda, and with the increase of the dose, the inhibition of mating became more obvious. When the dose exceeded 11 mg, the number of settings per hectare was At 500, the inhibition rate of mating was greater than 90%. At the same time, when the dose of cis-8-tetradecene acetate was 5 mg, the inhibition of mating rate was higher as the number of settings per hectare increased. Considering the cost, it is recommended that the content of cis-8-tetradecene ...
Embodiment 3
[0019] Example 3 Field release rate of Spodoptera frugiperda sex pheromone fascination agent
[0020] The lure core (cis-8-tetradecene acetate 5mg) prepared in Example 1 was selected, hung in the field, and the remaining cis-8-tetradecene acetate in the lure core was extracted every 15 days for a continuous period of time. Statistics for 4 months, and quantitative determination by gas chromatography. After 4 months, the content of cis-8-tetradecene acetate was still 1.54 mg ( figure 2 ), indicating that the field lasting period of the Spodoptera frugiperda sex pheromone fascination agent of the present invention is greater than 4 months, and can be used for the control of Spodoptera frugiperda.
[0021] The sex pheromone fascination agent of the invention can interfere with the mating communication between males and females of Spodoptera frugiperda, minimize the mating rate of adult insects, reduce the reproduction number, play the dual functions of interference and trapping...
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