Supercharge Your Innovation With Domain-Expert AI Agents!

Method for converting ustilaginoidea virens A by utilizing fungi and application of ustilaginoidea virens A

A rice aspergillus, biotransformation technology, applied in the biological field, can solve problems such as poisoning, internal organ lesions, and the decline of livestock and poultry growth and reproductive capacity.

Pending Publication Date: 2022-03-25
CHINA AGRI UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aspergillin is a water-soluble cyclic peptide with extensive toxicity, and it has toxic effects on rice seed germination, seedlings and callus growth; it can cause the decline of growth and reproductive ability of livestock and poultry and internal organ lesions; Tubulin assembly and cytoskeleton formation are also inhibited

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for converting ustilaginoidea virens A by utilizing fungi and application of ustilaginoidea virens A
  • Method for converting ustilaginoidea virens A by utilizing fungi and application of ustilaginoidea virens A
  • Method for converting ustilaginoidea virens A by utilizing fungi and application of ustilaginoidea virens A

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0098] Example 1, Radecinium A transformed into rice germin I and J in phosphate buffer having a pH value of 7 via a rigidae chlamycosporin NitaF10 cell-free extract

[0099] First, experimental method

[0100] 1, the preparation method of the rigidae coridine Nitaf10 cell-free extract (pH is 7) is taken as follows: Take the activated rigid burgurous-agar medium (PDA) to activate a good rigidaeencore Nitaf10 inoculated to 100 ml of potato-glucose - In the 250ml triangular bottle of culture solution (PDB), the amount of fungus inoculated is 3-4 6mm sized, oscillated in the shaker for 3-5 days, and the shake good fermentation liquid (bacterial liquid) was loaded into 50ml. In the round bottom centrifuge tube, at 4 ° C, 12000 × g, centrifuged for 20 minutes, to the supernatant, and then add 0.5 M phosphate buffer (PBS) having a pH of about 30 ml of a pH of about 30 ml to the syride precipitate. In the ultrasonic cleaner (100% power) ice bath, ultrasonic oscillate 150 times, 2 seconds...

Embodiment 2

[0123] Example 2, Rice Rutin A is converted to rice gemmi Mikin M via a rigidae chlorofacial Nitaf10 cell-free extract in a carbonate buffer having a pH 9.

[0124] First, experimental method

[0125] 1, the preparation method of the brurry chrobus Nitaf10 cell-free extract (pH is 9) is as follows: Take the active Night Black Coridine Shell Nitaf10 in the potato-glucose-agar medium (PDA) inoculated to 100 ml of potato-glucose - In the 250ml triangular bottle of culture solution (PDB), the amount of fungus inoculated is 3-4 6mm sized, oscillated in the shaker for 3-5 days, and the shake good fermentation liquid (bacterial liquid) was loaded into 50ml. In the round bottom centrifuge tube, at 4 ° C, 12000 × g, centrifuge for 20 minutes, to the supernatant, and then add 0.5 M carbonate buffer (CBS) having a pH of about 30 ml of a pH of about 30 ml to the hyphae precipitate. In the ultrasonic cleaner (100% power), the ultrasonic oscillation is about 150 times, and each oscillates for 2...

Embodiment 3

[0135] Example 3 Dynamic changes in consumption and transformed product rice germin I and J production amount in the process of rice gemis A biological conversion time in Rice Coridin A biogenesis

[0136] First, experimental method

[0137] 1. 1 of the first step in the first step in Example 1.

[0138] 2. 2 of the second step in the first step in Example 1.

[0139] 3. The reaction is sampled at the first 0H, 6H, 12H, 24H, 36H, 48H, and 72H, and the reaction is stopped with an equal volume of methanol, and the HPLC analysis is carried out after 1 ml of ultrapure water.

[0140] Second, the experiment

[0141] The results are shown in Table 5, and the results showed that the transformed product was generated in the biological extract of rice germs A, and two transformed products occurred in the biological extract of the rigidaeen shell Nitaf10. The content of the prolongation of rice germs is gradually reduced, and the amount of rice germin I and J in the transformant product is ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for converting ustilaginoidea virens A by utilizing fungi and application of the ustilaginoidea virens A. According to the method, an enzyme system in a cell-free extracting solution of the shells of the melanospora hirsuta is utilized, and in a buffer solution with the pH value of 7, the ustilaginoidea A is converted into the ustilaginoidea I and the ustilaginoidea J; in a buffer solution with the pH value of 9, the ustilaginoidea virens A is converted into ustilaginoidea virens I, J and M. Experiments show that compared with the ustilaginoidea A, the cytotoxic activity of the converted products of the ustilaginoidea I, J and M is generally reduced. The method for converting the ustilaginoidea virens A by utilizing the fungi is simple in process, and can effectively perform biotransformation detoxification of the ustilaginoidea virens A.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and more particularly to a method of transforming rice germs A with fungi and its application. Background technique [0002] Rice False Smut is a disease that is seriously harmful to rice in the world. The main symptoms are the formation of rice balls in rice spikes. Rice disease disease is the Rice Ghooprobacteria, and is well-behaved in Villosiclava Virens (Nakata) Tanaka & Tanaka, Nothing Nakaishoidea Virens (Cooke) TAKAHASHI. The disease not only affects the quality of rice, causing rice to reduce production, but also to toxins that have harmful to human animals, seriously threatening food security and human health. There have been three types of major fungal toxins in Rice Balls and Rice Stereotypes, namely rice gemmocin, rice green bacositis and sorbicillinoids. [0003] At present, the method of detoxification of fungal toxins is: physics detoxification, chemical detoxification and biological trans...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K5/107C12P21/02C12N1/06C12N1/14A62D3/02C12R1/645A62D101/26
CPCC07K5/1016C12P21/02C12N1/066C12N1/14A62D3/02A62D2101/26
Inventor 周立刚赖道万王明安李鹏匡宇
Owner CHINA AGRI UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com