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Freeze-dried composition for nucleic acid amplification

A composition and freeze-drying technology, applied in the field of nucleic acid amplification freeze-dried compositions, can solve problems such as long time consumption

Active Publication Date: 2022-03-25
VAZYME BIOTECH NANJING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, the vacuum freeze-drying technology has greatly increased the possibility of storage and transportation of PCR reagents at room temperature, but it takes a long time to prepare the freeze-dried products, and the storage stability, reaction performance and shape of the freeze-dried products are all due to freeze-drying. There are huge differences in different protective agents and excipients, so finding a suitable freeze-drying system for PCR reagents still faces many challenges

Method used

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  • Freeze-dried composition for nucleic acid amplification
  • Freeze-dried composition for nucleic acid amplification
  • Freeze-dried composition for nucleic acid amplification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] 1. Preparation of freeze-dried protection solution

[0045] Prepare 6 different lyoprotectant solutions according to Table 1, weigh and prepare each component according to Table 1, and finally use ddH 2 O was adjusted to 100 mL to obtain a 5× concentration lyoprotectant solution.

[0046] Table 1 Different lyoprotectant solutions

[0047]

[0048]

[0049] 2. Preparation of freeze-drying system

[0050] According to the above-mentioned 6 kinds of lyoprotectant solution schemes, 6 kinds of lyophilization systems were prepared according to Table 2.

[0051] Table 2 freeze-drying system

[0052] Add components add volume 5× freeze-dry protection solution 10 μL 2×PCRMix 25 μL wxya 2 o

15 μL total capacity 50μL

[0053] 3. Obtain freeze-dried product

[0054] The 6 freeze-dried systems prepared according to Table 2 were mixed and then freeze-dried according to Table 3 to obtain 6 freeze-dried products.

[0055] Table ...

Embodiment 2

[0064] According to the results of Example 1, the components of the lyoprotectant solution were further optimized and adjusted so that the lyoprotectant solution could effectively protect the form and performance of the lyophilized product.

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Abstract

The invention provides a freeze-dried composition, belongs to the technical field of biology, and particularly relates to a freeze-dried composition for nucleic acid amplification. The freeze-drying composition is good in freeze-drying form, reaction performance before and after freeze-drying can be kept free of obvious change, normal-temperature transportation and storage of freeze-drying products can be achieved, and adverse effects on reagent performance caused by repeated freeze-thawing of conventional reagents are avoided.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a freeze-dried composition for nucleic acid amplification. Background technique [0002] Polymerase Chain Reaction (Polymerase Chain Reaction), referred to as PCR, is a technology invented by American scientist KaryB.Mullis in 1985 to rapidly amplify a specific DNA fragment in vitro. The production of millions of copies of DNA, based on its advantages of rapidity, sensitivity and specificity, has been used in medical testing and clinical testing since 1989, and is still one of the commonly used methods in medicine and diagnostics. [0003] Although PCR technology is widely used, PCR reagents have extremely high requirements on storage temperature. Once the temperature is too high during storage or transportation, related reagents, especially protein materials such as enzymes, are easily inactivated, resulting in loss of reagent function; The cold chain transportation of reagents wil...

Claims

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Application Information

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IPC IPC(8): C12Q1/686C12N9/96C12N9/12
CPCC12Q1/686C12N9/96C12N9/1252C12Y207/07007C12Q2521/101C12Q2527/125
Inventor 聂俊伟瞿志鹏曹林王瑶闫雪莉
Owner VAZYME BIOTECH NANJING