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Enzyme-free blood glucose sensor microelectrode of Pt/Au nano-alloy modified acupuncture needle with cabbage structure and preparation of enzyme-free blood glucose sensor microelectrode

A nano-alloy, acupuncture needle technology, applied in the direction of nanotechnology, nanotechnology, nanotechnology, etc. for sensing, can solve the problems of low stability of the immobilized enzyme glucose sensor, and the enzyme activity is susceptible to temperature, pH, humidity, etc. , to achieve superior electrocatalytic performance, high sensitivity, and the effect of improving electrocatalytic activity

Active Publication Date: 2022-03-25
HANGZHOU DIANZI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the immobilized enzyme glucose sensor shows good selectivity and sensitivity for glucose detection, the enzyme activity is easily affected by temperature, pH, humidity, etc., which makes the stability of the immobilized enzyme glucose sensor not high.

Method used

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  • Enzyme-free blood glucose sensor microelectrode of Pt/Au nano-alloy modified acupuncture needle with cabbage structure and preparation of enzyme-free blood glucose sensor microelectrode
  • Enzyme-free blood glucose sensor microelectrode of Pt/Au nano-alloy modified acupuncture needle with cabbage structure and preparation of enzyme-free blood glucose sensor microelectrode
  • Enzyme-free blood glucose sensor microelectrode of Pt/Au nano-alloy modified acupuncture needle with cabbage structure and preparation of enzyme-free blood glucose sensor microelectrode

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Stainless steel acupuncture needles (0.25mm×44mm) were washed with absolute ethanol and ultrapure water for 5 minutes at an ultrasonic power of 100w, and then dried with nitrogen. The dried bare electrode (AN) was scanned by electron microscope, from SEM figure 1 It can be seen that the bare electrode surface is very smooth.

Embodiment 2

[0041] (1) Au / AN: the acupuncture needles after washing in Example 1 are used as working electrodes, Ag / AgCl is used as reference electrode, and Pt wire is used as counter electrode, and 0.2mol / L H 2 SO 4 The solution is an electrolyte, and a certain amount of HAuCl is added to the electrolyte 4 , such that HAuCl 4 The concentration is 0.75mmol / L. Then deposit at a constant potential of −8 V for 180 s, so that the AuNPs are deposited on the acupuncture needle electrodes.

[0042] (2) Pt / Au / AN: Au / AN is the working electrode, Ag / AgCl is the reference electrode, Pt wire is the counter electrode, and 0.4mol / L H 2 SO 4 The solution is an electrolyte, and a certain amount of H is added to the electrolyte 2 PtCl 6 , such that H 2 PtCl 6 The concentration is 1.25mmol / L. Then deposit at a constant potential of −0.8 V for 300 s, so that the PtNPs are deposited on the Au / AN electrode.

[0043] The prepared Au / AN electrode and Pt / Au / AN electrode were scanned with a scanning ele...

Embodiment 3

[0045] (1) Low-voltage preparation of Au / AN: the acupuncture needles washed in Example 1 were used as working electrodes, Ag / AgCl was used as reference electrode, and Pt wire was used as counter electrode, and 0.2mol / L H 2 SO 4 The solution is an electrolyte, and a certain amount of HAuCl is added to the electrolyte 4 , such that HAuCl 4 The concentration is 0.75mmol / L. Then deposit at a constant potential of −0.5 V for 180 s, so that the AuNPs are deposited on the acupuncture needle electrodes.

[0046] (2) High-voltage preparation of Au / AN: the acupuncture needles washed in Example 1 were used as working electrodes, Ag / AgCl was used as a reference electrode, and Pt wire was used as a counter electrode. 2 SO 4 The solution is an electrolyte, and a certain amount of HAuCl is added to the electrolyte 4 , such that HAuCl 4 The concentration is 0.75mmol / L. Then deposit at a constant potential of −8 V for 180 s, so that the AuNPs are deposited on the acupuncture needle elec...

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Abstract

The invention discloses an enzyme-free blood glucose sensor microelectrode of a Pt / Au nano-alloy modified acupuncture needle with a cabbage structure and a preparation method of the enzyme-free blood glucose sensor microelectrode. The method comprises the following steps: placing a stainless steel acupuncture needle serving as a working electrode, Ag / AgCl serving as a reference electrode and a Pt wire serving as a counter electrode in an H2SO4 solution containing HAuCl4, and depositing AuNPs on the acupuncture needle by adopting voltage deposition to obtain Au / AN; au / AN serves as a working electrode, Ag / AgCl serves as a reference electrode, a Pt wire serves as a counter electrode, the Au / AN, the Ag / AgCl and the Pt wire are placed in an H2SO4 solution containing H2PtCl6, PtNPs are deposited on the surface of AuNPs through voltage deposition, and a Pt / Au / AN electrode is obtained; the Pt / Au / AN electrode of the cabbage structure provides abundant electrochemical active sites for glucose oxidation, and the formed sensor shows good electrocatalytic activity, wide linear range and high selectivity and sensitivity for glucose oxidation.

Description

technical field [0001] The invention belongs to the field of electrochemical sensors, and relates to a Pt / Au nano-alloy modified acupuncture needle with a cabbage structure and a microelectrode for an enzyme-free blood sugar sensor and its preparation. Background technique [0002] In recent years, with the improvement of quality of life and the increase of life pressure, the incidence of diabetes has been rising steadily and gradually getting younger. Blood glucose detection is currently an important basis for clinical diagnosis of diabetes, so it is very important to develop a low-cost, highly sensitive, and highly selective glucose detection sensor. Electrochemical sensors have attracted much attention due to their high accuracy, good stability, and wide detection range, and have achieved certain results in the field of glucose detection. At present, the electrochemical sensors for glucose detection are mainly divided into two categories: immobilized enzyme glucose senso...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327B82Y15/00
CPCG01N27/3278B82Y15/00
Inventor 刘红英肖琪刘光献陈简单周真增张雨晴厉力华
Owner HANGZHOU DIANZI UNIV
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