High performance liquid chromatography for measuring content of sec-butylamine

A technology of high performance liquid chromatography and sec-butylamine, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of high cost, harsh processing requirements, complicated operation, etc., and achieve the effect of low cost and easy operation

Pending Publication Date: 2022-03-25
SHANDONG YANTAI AGRI SCI & TECH INST
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] There are many methods for the determination of sec-butylamine at home and abroad, including spectrophotometry, gas chromatography, gas chromatography-mass spectrometry, liquid chromatography, liquid chromatography-mass spectrometry, etc. Some methods are complicated to operate, and some methods are sensitive to samples. The pre-treatment requirements are harsh, and some methods use mass spectrometry with high cost

Method used

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  • High performance liquid chromatography for measuring content of sec-butylamine

Examples

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Embodiment 1

[0015] A high-performance liquid chromatography method for determining the content of sec-butylamine

[0016] Weigh 0.1g of iodine, dissolve it in 100mL of methanol, and dilute to 1000mL with water to prepare a 0.01% iodine solution with a flow rate of 0.3ml / min. The sec-butylamine solution to be tested was introduced into a high-performance liquid chromatograph through an autosampler, separated by a C18 chromatographic column, derivatized with an iodine solution at 60°C, and then measured at an ultraviolet wavelength of 270nm.

Embodiment 2

[0018] A high-performance liquid chromatography method for determining the content of sec-butylamine

[0019] Weigh 0.3g of iodine, dissolve it in 100mL of methanol, and dilute to 1000mL with water to prepare a 0.03% iodine solution with a flow rate of 0.3ml / min. The sec-butylamine solution to be tested was introduced into the high performance liquid chromatography through an autosampler, separated by a C18 chromatographic column, derivatized with an iodine solution at 70°C, and then measured at an ultraviolet wavelength of 280nm. For details, see figure 1 ,according to figure 1 It can be seen that the peak at about 3.2 min is the chromatographic peak derived from sec-butylamine.

Embodiment 3

[0021] A high-performance liquid chromatography method for determining the content of sec-butylamine

[0022] Weigh 0.5g of iodine, dissolve it with 100mL of methanol, and dilute to 1000mL with water to prepare a 0.05% iodine solution with a flow rate of 0.3ml / min. The sec-butylamine solution to be tested was introduced into a high-performance liquid chromatograph through an autosampler, separated by a C18 chromatographic column, derivatized with an iodine solution at 80°C, and then measured at an ultraviolet wavelength of 330nm.

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Abstract

The invention relates to a high performance liquid chromatography method for measuring the content of sec-butylamine, and belongs to the technical field of analytical chemistry. Iodine and methanol are used for preparing a 0.01-0.05% iodine solution; the method comprises the following steps: introducing a sec-butylamine solution to be detected into a high performance liquid chromatograph through an automatic sampler, separating through a C18 chromatographic column, deriving at 60-80 DEG C by using an iodine solution at a flow rate of 0.3 ml/min, and determining at an ultraviolet wavelength of 270-330 nm. According to the present invention, the sec-butylamine content determination is achieved by using the liquid chromatography post-column derivatization method, and the method has characteristics of simple operation and low cost, and is suitable for the sec-butylamine content determination.

Description

technical field [0001] The invention relates to the technical field of analytical chemistry, in particular to a high-performance liquid chromatography method for determining the content of sec-butylamine. Background technique [0002] S-butylamine is a protective fungicide, which has fungicidal activity against various fungi, and is widely used in the freshness preservation of green peppers, citrus, garlic sprouts, grapes and other vegetables and fruits during transportation and storage. Studies have found that sec-butylamine is harmful to the human body to a certain extent. The United States has set its hazard level to human health as level three, and has made regulations on its use range and allowable residue. Japan also stipulated the maximum allowable residue of sec-butylamine in 2006. [0003] There are many methods for the determination of sec-butylamine at home and abroad, including spectrophotometry, gas chromatography, gas chromatography-mass spectrometry, liquid c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72
Inventor 兰丰鹿泽启李瑞丽王建萍孙林
Owner SHANDONG YANTAI AGRI SCI & TECH INST
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