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Thyroid nodule benign and malignant related marker and application thereof

A thyroid nodule and homogeneity technology is applied in the field of benign and malignant thyroid nodules related markers, which can solve the problems of positive predictive value, diagnostic accuracy, low sensitivity and specificity, etc., to improve sensitivity and specificity, and facilitate promotion. Effects that are easy to apply, result in

Pending Publication Date: 2022-04-05
SINGLERA GENOMICS (SHANGHAI) LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, up to 20% of the nodules in cytological examination results are indeterminate thyroid nodules. These nodules need to be combined with molecular testing, but the positive predictive value of molecular testing products for thyroid cancer on the market, Low diagnostic accuracy, sensitivity, and specificity

Method used

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  • Thyroid nodule benign and malignant related marker and application thereof
  • Thyroid nodule benign and malignant related marker and application thereof
  • Thyroid nodule benign and malignant related marker and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0134] Example 1, verifying the relationship between benign and malignant thyroid nodules and tissue genome DNA methylation.

[0135] The applicant performed genomic DNA methylation-specific PCR on the tissues of 20 patients with thyroid cancer and 20 patients with benign thyroid nodules, and found that the genes SLC16A3, PRR15, CDH1, TSHR and GRIA2 were present in the genomes of thyroid cancer and benign thyroid nodules cases. DNA methylation levels are different, the results are as follows figure 1 shown. The specific experimental steps are as follows:

[0136] 1.1, Sample Preparation

[0137] Use QIAamp DNA Mini Kit (QIAGEN, catalog number: 51304) to extract DNA from 20 cases of thyroid cancer and 20 cases of benign thyroid nodules (thyroid nodule biopsy tissue); use QubitTM dsDNA HS Assay Kit (Thermo, catalog number: Q32854 ) to detect the concentration of DNA; use 1% agarose gel electrophoresis for quality inspection.

[0138] 1.2, DNA Transformation

[0139] Use the...

Embodiment 2

[0186] Example 2, verifying the relationship between benign and malignant thyroid nodules and cfDNA methylation in plasma

[0187] The applicant performed methylation-specific PCR on the plasma cfDNA of 196 patients with thyroid cancer and 148 patients with benign thyroid nodules, and found that SLC16A3, PRR15, CDH1, TSHR and GRIA2 genes were significantly higher in plasma cfDNA of thyroid cancer and benign thyroid nodules. There are differences in methylation levels, with results such as image 3 shown. The specific experimental steps are as follows:

[0188] 2.1, sample preparation (same as 1.1 sample preparation)

[0189] 2.2, DNA transformation (same as 1.2 DNA transformation)

[0190] 2.3, PCR mixture preparation (same as 1.3 PCR mixture preparation)

[0191] 2.4, fluorescent quantitative PCR (same as 1.4 fluorescent quantitative PCR)

[0192] 2.5, analysis of test results

[0193] Utilize the following formula and the formula in embodiment 2 to calculate the methylat...

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Abstract

The invention provides a composition for identifying benign and malignant thyroid nodules. The composition comprises a nucleotide sequence of an SLC16A3 gene, a nucleotide sequence of a PRR15 gene, a nucleotide sequence of a CDH1 gene, a nucleotide sequence of a TSHR gene, a nucleotide sequence of a GRIA2 gene, a nucleotide sequence of a reference gene ACTB and a nucleotide sequence of an optional reference gene in methylation regions related to benign and malignant thyroid nodules in a sample. By analyzing the methylation level of the methylation marker in tissue or plasma, benign and malignant thyroid nodules of a subject can be diagnosed.

Description

technical field [0001] The invention belongs to the field of molecular auxiliary diagnosis, and relates to markers related to benign and malignant thyroid nodules and applications thereof. Background technique [0002] Thyroid nodules are solid or fluid-filled lumps that form in the thyroid after abnormal growth of thyroid cells. They can move up and down with swallowing along with the thyroid, a small gland at the base of the neck, just above the breastbone . Although most thyroid nodules are not serious and cause no symptoms, some thyroid nodules are cancerous. In order to diagnose and treat thyroid cancer earlier and reduce unnecessary surgical treatment, a highly sensitive and specific in vitro diagnostic method is urgently needed to differentiate benign from malignant thyroid nodules. [0003] The American Thyroid Association (ATA) defines a thyroid nodule as a discrete lesion on the thyroid gland. With the help of imaging examination, it can be observed that the nodu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12N15/11C12N15/12G16B30/10
Inventor 刘蕊苏明扬何其晔
Owner SINGLERA GENOMICS (SHANGHAI) LTD
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