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TA-siRNA nanogel as well as preparation method and application thereof

A nano-gel and solution technology, which is applied in the direction of pharmaceutical formulations, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve the problem of low activity, stability, specific performance, and poor curative effect of siRNA , unable to achieve efficient and safe siRNA, etc., to achieve the effect of promoting contact probability, achieving delivery and cell internalization, and structural stability

Active Publication Date: 2022-04-08
NORTHWEST UNIV(CN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Most early preclinical treatments using siRNA were discontinued due to poor efficacy or large off-target effects
One of the major issues interfering with gene therapy is the inability to efficiently and safely deliver siRNAs to desired tissues and cells, resulting in poor performance in terms of activity, stability, specificity, and reducing potential off-targets

Method used

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  • TA-siRNA nanogel as well as preparation method and application thereof
  • TA-siRNA nanogel as well as preparation method and application thereof
  • TA-siRNA nanogel as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] This embodiment provides a preparation method of TA-siRNA nanogel, comprising:

[0041] Step 1, siRNA is dissolved in the PBS buffer solution, obtains the siRNA solution of 0.2OD / mL; The PBS buffer solution is the PBS buffer solution without RNase; The PBS buffer solution without RNase can be, for example, pH7.4 Phosphate buffer solution containing 1.44gNa per 1000mL of the phosphate buffer solution 2 HPO 4 , 0.24gKH 2 PO 4 , 8.0gNaCl and 0.2gKCl;

[0042] Step 2, dissolving tannic acid (TA) in a PBS buffer solution to obtain a 0.2% TA solution by mass percentage; the PBS buffer solution is the same as the PBS buffer solution in step 1;

[0043] Step 3. Stir 1 mL of the siRNA solution described in Step 1 at 0°C for 10 min, drop in 1 mL of the TA solution described in Step 2, and continue stirring for 20 min; the stirring is carried out in a magnetic stirrer, and the stirring The rate is 200rpm; the rate of dropping TA solution is 1mL / min;

[0044] Step 4, continue...

Embodiment 2

[0047]This example is the same as Example 1, except that in Step 1, the concentration of the siRNA solution is 4OD / mL.

Embodiment 3

[0049] This example is the same as Example 1, except that in Step 2, the mass percentage of TA solution is 1%.

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PUM

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Abstract

The invention discloses TA-siRNA nanogel as well as a preparation method and application of the TA-siRNA nanogel. The method comprises the following steps: dropwise adding a TA solution into an siRNA solution, and stirring for 5-120 minutes at the temperature of 0-40 DEG C to obtain TA-siRNA nanogel. According to the method, the nanogel is obtained by utilizing the hydrogen-bond interaction of tannic acid and siRNA, and phenolic hydroxyl groups in the tannic acid are combined with cell membrane surface proteins, so that cells are adhered, cell endocytosis is promoted, cell internalization is realized, and the TA-siRNA nanogel is obtained. The compound has the advantages of remarkable cell internalization promoting effect, simple preparation method, stable product structure and high biological safety.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, and in particular relates to a TA-siRNA nano gel and a preparation method and application thereof. Background technique [0002] Gene therapy is a promising therapeutic platform for more precise and personalized treatment of various life-threatening diseases by targeting disease-causing genes with specific sequences, which can down-regulate, enhance or correct gene expression. The principle of its treatment is to form an interference complex (RISC) through the combination of interference RNA and protein. The complex RISC targets the target gene (such as messenger RNA), degrades it, and knocks out the downstream protein expression of the gene to achieve disease treatment. Small interfering RNA (siRNA) is formed by cutting double-stranded RNA by endonuclease. After entering the cell, it forms a sense strand and an antisense strand under the action of a helicase. The antisense strand and the po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/69A61K47/54A61K31/7088
CPCY02A50/30
Inventor 范代娣雷桓
Owner NORTHWEST UNIV(CN)
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