Culture device for multi-cell co-culture and cell culture method

Abstract

The embodiment of the invention discloses a culture device for multi-cell co-culture and a cell culture method. The culture device comprises a first main flow channel layer, a porous film and a second main flow channel layer, the first main flow channel layer comprises a multi-dimensional cell culture area, the second main flow channel layer comprises a hydrogel scaffold culture area and a cell mass-shaped culture area, the hydrogel scaffold culture area and the cell mass-shaped culture area are nested, the cell mass-shaped culture area is arranged in the hydrogel scaffold culture area, and the cell mass-shaped culture area is arranged in the hydrogel scaffold culture area. The plane height of the hydrogel scaffold culture area is higher than that of the cell cluster culture area, the porous film is located below the first main flow channel layer, the porous film is located above the second main flow channel layer, and the position of the porous film corresponds to the multi-dimensional cell culture area and the hydrogel scaffold culture area. The multi-dimensional cell culture area and the hydrogel support culture area are divided through the porous film, and information interconnection can be achieved. And a bionic extracellular matrix is simulated through a hydrogel scaffold culture area, so that the bionic performance of an in-vitro hepatogenesis model and a pathological model can be improved.

Description

technical field [0001] The invention relates to the field of cell culture chip manufacturing, in particular to a multi-cell co-cultivation culture device and a cell culture method. Background technique [0002] Currently, studies on the pathogenesis of liver diseases and drug verification are mainly based on simple two-dimensional cell models and animal models. Under two-dimensional culture conditions, liver cells rapidly lose their phenotype and cell function due to the lack of a microenvironment similar to that in vivo, and the effect of drugs is far from that of actual use, resulting in increased drug development costs and increased cycles. The use of animal models is limited due to interspecies differences, ethical issues, and high investment costs. The microenvironment includes the cellular microenvironment composed of different cells and the physiological microenvironment composed of organ structures, such as nutrients formed by blood flow, oxygen concentration gradie...

AI Technical Summary

Problems solved by technology

At present, the mainstream cell culture technologies such as Petri dish culture, Trans-well culture, cell mass culture, etc. It is difficult for such culture technologies to cover both the cell microenvironment and the structural microenvironment, so the final growth state of the cells is very different, which affects the drug determination results

[0003] The existing liver sinusoidal models have not been able to highly simulate the stiffness and structure of tissues in vitro. Although recent studies have used bioprinting technology to establish liver sinusoidal models, they only reflect changes in the stiffness of liver fibrosis and do not have good structural bionics. sex

Images