Lactobacillus sake capable of being co-aggregated with helicobacter pylori and application of lactobacillus sake

A technology of Lactobacillus sake and viable count, applied in the field of microorganisms, can solve problems such as adverse reactions, patient injury, drug-resistant Helicobacter pylori strains, etc.

Active Publication Date: 2022-04-12
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the frequent use of antibiotics, it is easy to lead to the emergence of drug-resistant Helicobacter pylori strains. At the same time, in the process of using triple or quadruple th

Method used

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  • Lactobacillus sake capable of being co-aggregated with helicobacter pylori and application of lactobacillus sake
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  • Lactobacillus sake capable of being co-aggregated with helicobacter pylori and application of lactobacillus sake

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Example 1: Screening and identification of Lactobacillus sake CCFM1199

[0088] 1. Screening

[0089] Taking distiller's grains from Maotai Town, Renhuai City, Zunyi City, Guizhou Province as samples, the samples were pretreated and stored in a -80°C refrigerator in about 30% glycerin. Enrich in 5mL LBS medium for 8 hours, dilute the enriched solution with 0.9% normal saline, select the appropriate gradient dilution solution and spread it on the LBS solid medium, culture it at 37°C for 48 hours, pick typical colonies to the MRS plate Purify by overlining, pick a single colony and transfer to liquid MRS liquid medium for enrichment, and store in 30% glycerol to obtain strain CCFM1199.

[0090] 2. Identification

[0091] The genome of the screened Lactobacillus sake was extracted, and its 16S rDNA was amplified and sequenced (completed by Shanghai Sangon Bioengineering Co., Ltd.). After sequencing analysis, the 16S rDNA sequence of the strain was shown in SEQ ID NO.1, ...

Embodiment 2

[0093] Embodiment 2: the cultivation of Lactobacillus sake CCFM1199

[0094] The Lactobacillus sake CCFM1199 prepared in Example 1 was inserted into the MRS solid medium, and after cultivating at 37° C. for 48 hours, its colony was observed, and it was found that its colony was milky white and rounded, with a smooth, moist surface and neat edges (such as Figure 10 shown).

[0095] Insert the Lactobacillus sake CCFM1199 prepared in Example 1 into the MRS liquid medium and culture it at 37°C for 24 hours. During the cultivation process, measure the OD of the culture solution with a microplate reader at intervals of 4 hours 600 , it was found that Lactobacillus sake reached a stable growth period after 16-24 hours of culture, and the results were as follows figure 1 shown.

Embodiment 3

[0096] Example 3: Co-aggregation of Lactobacillus sake to Helicobacter pylori

[0097] Specific steps are as follows:

[0098] (1) Culture of Helicobacter pylori:

[0099] After the Helicobacter pylori was streaked on the Columbia blood plate, it was cultured in a three-gas incubator (85% N) at 37°C. 2 , 10% CO 2 , 5% O 2 ) was cultivated for 3 days to obtain a single colony; pick a single colony and inoculate it in the BHI medium containing 5% (v / v) fetal bovine serum, in a three-gas incubator at 37°C (85% N 2 , 10% CO 2 , 5% O 2 ) was cultivated for 4 days to obtain the seed solution; the seed solution was inoculated in the BHI medium with an inoculum size of 2% (v / v), and was inoculated in a three-gas incubator at 37° C. (85% N 2 , 10% CO 2 , 5% O 2 ) was cultured for 4 days to obtain the activated third-generation Helicobacter pylori bacterium liquid; the Helicobacter pylori bacterium liquid was centrifuged at 8000 g for 10 min to obtain the Helicobacter pylori tha...

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Abstract

The invention discloses lactobacillus sake capable of being co-aggregated with helicobacter pylori and application of the lactobacillus sake, and belongs to the technical field of microorganisms and the technical field of medicines. The invention provides a lactobacillus sake 1199 strain, which is preserved in the Guangdong Province Microbial Culture Collection Center, the preservation number is GDMCC No: 61953, and the preservation date is September 26, 2021. The lactobacillus sake 1199 strain has the advantages that the lactobacillus sake 1199 strain is preserved in the Guangdong Province Microbial Culture Collection Center, the preservation number is GDMCC No: 61953; the lactobacillus sake CCFM1199 bacterial suspension and helicobacter pylori can generate a co-aggregation effect in artificial gastric juice (pH = 4), and the adhesive force of the helicobacter pylori to AGS cells can be remarkably reduced; the lactobacillus sake CCFM1199 has a huge application prospect in the preparation of products (such as food or medicines and the like) for preventing and/or treating helicobacter pylori infection.

Description

technical field [0001] The invention relates to a strain of Lactobacillus sake capable of co-accumulating with Helicobacter pylori and an application thereof, belonging to the technical field of microbes and the technical field of medicine. Background technique [0002] Helicobacter pylori (H. pylori) is a helical or S-shaped microaerophilic Gram-negative strain, which is one of the main causes of gastritis, peptic ulcer, and even gastric cancer. Helicobacter pylori has a very high infection rate and infection rate. About 50% of the people in the world suffer from this disease, because its infection status is closely related to race, socioeconomic and health conditions, etc. Therefore, the infection rate in developing countries Higher than developed countries, what's more, it can reach 80%-90%. [0003] Currently, triple or quadruple therapy combined with antibiotics is mainly used in the treatment of Helicobacter pylori. However, due to the frequent use of antibiotics, it...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A61P31/04A23C9/123A23L11/50A23L33/00A23L2/38A23L19/20C12R1/225
CPCY02A50/30
Inventor 翟齐啸张华月陈卫于雷雷田丰伟陆文伟崔树茂王刚赵建新张灏
Owner JIANGNAN UNIV
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