Osteoarthritis related gene screening and function analysis method

Abstract

The invention discloses an osteoarthritis related gene screening and function analysis method, which comprises the following steps: searching an osteoarthritis related gene expression chip result from a GEO database, and obtaining a gene expression result; searching the same differential genes in the data sets of the two chips by utilizing a Venn graph; and carrying out gene enrichment function analysis by utilizing a bioinformatics technology. According to the method, expression difference genes are downloaded by utilizing multiple online databases, genes for co-expression difference in two chip data sets are searched, bioinformatics analysis is carried out on the co-expression difference genes, and meaningful exploration and basis are provided for osteoarthritis synovial tissue related marker screening, molecular pathogenesis and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for screening and functional analysis of osteoarthritis-related genes. Background technique [0002] Osteoarthritis (OA) is a relatively common chronic arthritic disease clinically, which is characterized by progressive cartilage degeneration, synovial inflammation and bone remodeling. Accumulating evidence indicates that synovial inflammation and subsequent pro-inflammatory and destructive mediators play an important role in the pathogenesis and progression of OA. The most typical clinical symptoms of patients with osteoarthritis are joint pain, swelling, and limited joint function; OA has a high incidence, affecting the quality of life of about 28% of middle-aged and elderly people, and the incidence increases with the age of patients Elevated, the incidence rate of women is higher than that of men. In recent years, with the intensification of aging in China, the proportio...

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Problems solved by technology

The initial stage of OA is hidden, and early diagnosis of OA is very important; the existing methods for examining articular cartilage mainly include X-ray, CT, arthrography, arthroscopy, and MR routine imaging sequences (SE, FSE, GRE, 3I) ), etc., are all based on cartilage morphological changes: although X-ray and CT can show bone destruction and erosion, they cannot directly show synovial exudation, synovial hyperplasia and pannus formation, articular cartilage destruction and ligament damage in OA. , tendon abnormalities and other pathological changes, thus delaying early treatment and losing the value of early diagnosis; for OA patients with mild or lack of active inflammation, MRI is not more sensitive than other imaging techniques. Due to the influence of shadow and other factors, MRI diagnosis of OA also has a certain false positive rate.

Therefore, the sensitivity and accuracy of the existing OA imaging detection methods are not good, and there is a lag in the early diagnosis of OA.

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