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Bigeminy fluorescent quantitative detection reagent for canine coronavirus

A fluorescent quantitative detection technology for coronaviruses, applied in fluorescence/phosphorescence, measuring devices, instruments, etc., can solve problems such as inability to detect canine coronavirus and parvovirus at the same time, inability to effectively dilute animal feces or vomit, poor detection results, etc. , to achieve good results, improve the detection rate, and detect accurately

Pending Publication Date: 2022-04-15
山东鑫诺生物工程股份有限公司
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the deficiencies in the prior art, the present invention provides a dual fluorescence quantitative detection reagent for canine coronavirus, which solves the problem that the sample diluent used in the traditional colloidal gold method cannot effectively dilute animal feces or vomit, and cannot detect canine coronavirus and parvovirus. Simultaneous detection, the problem of poor detection effect

Method used

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Embodiment 1

[0027] The embodiment of the present invention provides canine coronavirus dual fluorescence quantitative detection reagent, including CPV / CCV dual detection reagent card and sample diluent;

[0028] The CPV / CCV dual detection reagent card includes the reagent card body and the sample wells, binding pads, and NC membranes arranged sequentially on the reagent card body. The binding pad contains fluorescent microspheres labeled with fluorescent light, and the binding pad is connected to one end of the NC membrane. , There is a T line in the middle of the NC membrane, and a C line at the other end of the NC membrane, and the sample hole is used to add sample diluent;

[0029] Fluorescent microspheres include microspheres conjugated to canine coronavirus antibody and microspheres conjugated to canine parvovirus antibody;

[0030] It also includes a pipette tip and a cotton swab, the pipette tip is used to slowly drop the sample diluent into the sample hole of the reagent card body...

Embodiment 2

[0045] The embodiment of the present invention provides canine coronavirus dual fluorescence quantitative detection reagents, including multiple CPV / CCV dual detection reagent cards, multiple sample diluents, multiple pipette tips, and multiple cotton swabs;

[0046] The CPV / CCV dual detection reagent card includes the reagent card body and the sample wells, binding pads, and NC membranes arranged sequentially on the reagent card body. The binding pad contains fluorescent microspheres labeled with fluorescent light, and the binding pad is connected to one end of the NC membrane. , There is a T line in the middle of the NC membrane, and a C line at the other end of the NC membrane. The sample hole is used to add sample diluent; the fluorescent microspheres include microspheres coupled with canine coronavirus antibody and canine parvovirus antibody. Microspheres;

[0047] The pipette tip is used to slowly drop the sample diluent into the sample hole of the reagent card body, and t...

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Abstract

The invention provides a bigeminy fluorescent quantitative detection reagent for canine coronavirus, and relates to the technical field of virus detection. The canine coronavirus bigeminy fluorescent quantitative detection reagent comprises a CPV / CCV bigeminy detection reagent card and a sample diluent, the CPV / CCV bigeminy detection reagent card comprises a reagent card body, and a sample adding hole, a combination pad and an NC membrane which are sequentially arranged on the reagent card body, the combination pad contains fluorescent microspheres labeled by fluorescence, and the fluorescent microspheres comprise microspheres coupled with a canine coronavirus antibody and microspheres coupled with a canine parvovirus antibody. The concentration of canine parvovirus / canine coronavirus in canine faeces is detected by adopting a double-antibody sandwich method, a test sample is added into a diluent to be uniformly mixed and dropwise added into a sample adding hole, and an antigen can be diffused onto a combination pad along with the sample diluent and is combined with a fluorescently-labeled antibody, so that simultaneous detection of canine coronavirus and parvovirus is realized; detection is accurate and the effect is good.

Description

technical field [0001] The invention relates to the technical field of virus detection, in particular to a double fluorescent quantitative detection reagent for canine coronavirus. Background technique [0002] Canine coronavirus is a single-stranded positive-strand RNA virus with 6 to 7 polypeptides, 4 of which are glycopeptides, and does not contain RNA polymerase and neuraminidase. Canine coronavirus (Canine Coronavirus, CCV) is a serious hazard Source of viral infectious diseases in kennel industry, economic animal breeding industry and wildlife protection. Canine parvovirus (Canine Parvovirus, CPV) was isolated from the feces of sick dogs suffering from enteritis in 1978 at the same time by Kelly in Australia and Thomson in Canada. Since the discovery of the virus, it has spread all over the world and is the most important cause of harm to dogs. one of the most severe infectious diseases. Canine coronavirus is often co-infected with parvovirus, which constitutes a pan...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N33/569G01N33/533G01N33/543
Inventor 刘忠刘青平刘春丽王金龙唐妍
Owner 山东鑫诺生物工程股份有限公司
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